Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
1.
Microbiol Resour Announc ; 10(5)2021 Feb 04.
Article in English | MEDLINE | ID: mdl-33541872

ABSTRACT

The family Microbacteriaceae represents a diverse and important group of soil bacteria in the phylum Actinobacteria Here, we report the genome sequence of a soil Microbacteriaceae strain, Protaetiibacter sp. strain SSC-01, the second putative species of the genus. Iron acquisition and xylose metabolism are central pathways identified in the annotated genome.

2.
J Forensic Sci ; 65(4): 1310-1314, 2020 Jul.
Article in English | MEDLINE | ID: mdl-31985836

ABSTRACT

In forensic science, fingerprints are a common source of evidentiary information. However, latent examination is not always successful and trace human DNA cannot always be obtained. Thus, examining the fingerprint microbiome may offer a suitable alternative to more traditional methods of forensic identification. The Zymo Research ZR Bacterial/Fungal DNA MicroPrep™ Kit, Qiagen QIAmp® DNA Mini Kit, Promega Wizard® Genomic DNA Purification Kit, and the MPBio FastDNA® Spin Kit were compared for their ability to yield a sufficient amount of bacterial DNA for next-generation sequencing in order to obtain a microbiome profile. Prints were deposited onto slides, allowed to sit for up to 1 month, and total DNA isolated and quantified using each kit. The kit from Zymo Research yielded the most concentrated DNA sample (0.0084 ng/µL) in the least amount of time as compared to other kits examined. Although this amount of DNA was far below the recommended DNA concentration threshold recommended for next-generation sequencing, a microbiome profile was successfully obtained. As interest in using the microbiome of an individual as a forensic tool continues to increase, there is the possibility that the microbiome of a fingerprint could complement traditional human DNA profiling in the future. This study provides evidence that trace amounts of bacterial DNA from fingerprints is quantifiable and sufficient for microbiome analysis.


Subject(s)
DNA Fingerprinting/instrumentation , DNA, Bacterial/genetics , Dermatoglyphics , DNA, Bacterial/isolation & purification , DNA, Fungal/genetics , DNA, Fungal/isolation & purification , High-Throughput Nucleotide Sequencing , Humans , Microbiota , Polymerase Chain Reaction
3.
J Zoo Wildl Med ; 47(3): 883-885, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27691955

ABSTRACT

Gypsum caves found throughout the Red Hills of Kansas have the state's most diverse and largest population of cave-roosting bats. White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, which threatens all temperate bat species, has not been previously detected in the gypsum caves as this disease moves westward from the eastern United States. Cave soil was obtained from the gypsum caves, and using the polymerase chain reaction, a 624-nucleotide DNA fragment specific to the Type 1 intron-internal transcribed spacer region of the 18S rRNA gene from Pseudogymnoascus species was amplified. Subsequent cloning and DNA sequencing indicated P. destructans DNA was present, along with 26 uncharacterized Pseudogymnoascus DNA variants. However, no evidence of WNS was observed in bat populations residing in these caves.


Subject(s)
Ascomycota/genetics , Chiroptera/microbiology , RNA, Ribosomal, 18S/genetics , Animals , DNA, Fungal/genetics , DNA, Ribosomal Spacer/genetics , Kansas , RNA, Fungal/genetics , Soil Microbiology
4.
J Zoo Wildl Med ; 44(3): 666-71, 2013 Sep.
Article in English | MEDLINE | ID: mdl-24063095

ABSTRACT

Free-ranging Red-eared Sliders (Trachemys scripta elegans) were captured from farm ponds located in the Flint Hills of Kansas and a zoo pond in Emporia, Kansas, USA, to evaluate their enteric bacterial flora and associated antibiotic resistance. Bacteria obtained from cloacal swabs were composed of six different Gram-negative genera. Although antibiotic resistance was present in turtles captured from both locations, 40 and 49% of bacteria demonstrated multiple antibiotic resistance to four of the antibiotics tested from the zoo captured and Flint Hills ponds turtles, respectively. These data illustrate environmental antibiotic resistance is widespread in the bacterial flora obtained from Red-eared Sliders in east central Kansas.


Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial , Gram-Negative Bacteria/drug effects , Gram-Negative Bacteria/isolation & purification , Turtles/microbiology , Animals , Animals, Wild , Animals, Zoo
5.
PLoS One ; 7(8): e43203, 2012.
Article in English | MEDLINE | ID: mdl-22905235

ABSTRACT

BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) strains are the leading bacterial cause of diarrhea to humans and farm animals. These ETEC strains produce heat-labile toxin (LT) and/or heat-stable toxins that include type I (STa), type II (STb), and enteroaggregative heat-stable toxin 1 (EAST1). LT, STa, and STb (in pigs) are proven the virulence determinants in ETEC diarrhea. However, significance of EAST1 in ETEC-associated diarrheal has not been determined, even though EAST1 is highly prevalent among ETEC strains. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we constructed E. coli strains to express EAST1 toxin as the only toxin and studied them in cell lines and five-day old gnotobiotic piglets to determine significance of EAST1 toxin. Data from in vitro studies indicated that EAST1 did not stimulate an increase of intracellular cyclic AMP or GMP levels in T-84 cells or porcine cell line IPEC-J2, nor did it enhance LT or STa toxin of ETEC strains in stimulation of cAMP or cGMP in T-84 cells. In addition, 5-day old gnotobiotic pigs challenged with E. coli strains expressing EAST1 as the only toxin did not developed diarrhea or signs of clinical disease during 72 h post-inoculation. CONCLUSION/SIGNIFICANCE: Results from this study indicated that EAST1 alone is not sufficient to cause diarrhea in five-day old gnotobiotic pigs, and suggest that EAST1 likely is not a virulence determinant in ETEC-associated diarrhea.


Subject(s)
Bacterial Toxins/biosynthesis , Cyclic AMP/metabolism , Cyclic GMP/metabolism , Diarrhea/microbiology , Enterotoxigenic Escherichia coli/metabolism , Enterotoxins/physiology , Animals , Cell Line , Cloning, Molecular , Enterotoxins/biosynthesis , Enterotoxins/metabolism , Enzyme-Linked Immunosorbent Assay/methods , Escherichia coli Proteins , Plasmids/metabolism , Prevalence , Swine , Time Factors , Virulence
6.
FEBS Lett ; 579(3): 728-32, 2005 Jan 31.
Article in English | MEDLINE | ID: mdl-15670836

ABSTRACT

The enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the penultimate step of the shikimate pathway, and is the target of the broad-spectrum herbicide glyphosate. Kinetic analysis of the cloned EPSPS from Staphylococcus aureus revealed that this enzyme exerts a high tolerance to glyphosate, while maintaining a high affinity for its substrate phosphoenolpyruvate. Enzymatic activity is markedly influenced by monovalent cations such as potassium or ammonium, which is due to an increase in catalytic turnover. However, insensitivity to glyphosate appears to be independent from the presence of cations. Therefore, we propose that the Staphylococcus aureus EPSPS should be classified as a class II EPSPS. This research illustrates a critical mechanism of glyphosate resistance naturally occurring in certain pathogenic bacteria.


Subject(s)
Alkyl and Aryl Transferases/drug effects , Glycine/analogs & derivatives , Glycine/pharmacology , Staphylococcus aureus/enzymology , 3-Phosphoshikimate 1-Carboxyvinyltransferase , Alkyl and Aryl Transferases/genetics , Alkyl and Aryl Transferases/isolation & purification , Base Sequence , Cloning, Molecular , DNA Primers , Electrophoresis, Polyacrylamide Gel , Kinetics , Polymerase Chain Reaction , Glyphosate
7.
FEMS Microbiol Lett ; 215(2): 249-53, 2002 Oct 08.
Article in English | MEDLINE | ID: mdl-12399042

ABSTRACT

Fifty-eight clinical Salmonella typhi strains isolated from patients suspected of suffering from typhoid fever were obtained at the Korle-Bu Teaching Hospital and the Noguchi Memorial Institute for Medical Research, both located in Ghana, Africa. Each isolate was examined for susceptibility to ampicillin, chloramphenicol, streptomycin, tetracycline, and trimethoprim/sulfamethoxazole by the disk diffusion assay. Five of the isolates were resistant to all five antibiotics while 10 isolates were resistant to ampicillin, chloramphenicol, and trimethoprim/sulfamethoxazole, which are considered 'first line' antibiotics in the treatment of typhoid fever. Thirty-four isolates were resistant to at least one of the antibiotics tested and 62% of these isolates possessed conjugable plasmids belonging to incompatibility group IncHI. Ninety percent of the conjugable plasmids conferred a multiple drug-resistant phenotype on the strains harboring them. Additionally, 14 strains contained plasmids that were transformable and six of them encoded multiple drug resistance. Our findings indicate that multiple drug resistance to the 'first line' antibiotics in S. typhi may be more prevalent in Africa than previously thought.


Subject(s)
Drug Resistance, Multiple, Bacterial/genetics , Plasmids/genetics , Salmonella typhi/drug effects , Anti-Bacterial Agents/pharmacology , Conjugation, Genetic , Gene Transfer, Horizontal , Ghana , Humans , Microbial Sensitivity Tests , Salmonella typhi/isolation & purification , Transformation, Bacterial , Typhoid Fever/microbiology
8.
Avian Dis ; 46(3): 708-12, 2002.
Article in English | MEDLINE | ID: mdl-12243537

ABSTRACT

Serologic testing by the serum plate agglutination (SPA) procedure was performed to detect the presence of cross-reacting antibodies to Mycoplasma meleagridis, Mycoplasma synoviae, and Mycoplasma gallisepticum in lesser prairie-chickens (Tympanuchus pallidicinctus) trapped over a 2-yr period in Finney and Kearny counties of southwestern Kansas. Sera examined from birds (n = 50) obtained in March-April 2000 tested positive for M meleagridis, M. synoviae, and M. gallisepticum at levels of 6%, 10%, and 10%, respectively, for the population examined. Mycoplasma meleagridis antibodies were detected in 3 samples (2.7%), M. synoviae antibodies in 2 samples (1.7%), and M. gallisepticum antibodies in 3 samples (2.7%) from birds (n = 112) collected in March-April 2001. Data obtained by SPA can result in false positives and should be verified by additional procedures such as the hemagglutination-inhibition test. Low amounts of sera prohibited this additional testing. Thus, the positive SPA results should be considered presumptive for the presence of Mycoplasma antibodies. Although Mycoplasma antibodies have been detected in wild turkeys (Meleagris gallopavo) from Kingman and Butler counties in Kansas, this report is the first of possible mycoplasmosis in Finney and Kearny counties, Kansas. All birds testing positive by this procedure should be considered as potential carriers of Mycoplasma and should not be used in relocation efforts.


Subject(s)
Antibodies, Bacterial/blood , Bird Diseases/epidemiology , Mycoplasma Infections/veterinary , Mycoplasma/immunology , Agglutination Tests/veterinary , Animals , Animals, Wild , Bird Diseases/microbiology , Birds , Cross Reactions , Female , Hemagglutination Inhibition Tests/veterinary , Kansas/epidemiology , Male , Mycoplasma Infections/epidemiology , Mycoplasma Infections/microbiology , Prevalence
SELECTION OF CITATIONS
SEARCH DETAIL
...