Aqueous synthesis of red fluorescent l-cysteine functionalized Cu2S quantum dots with potential application as an As(iii) aptasensor.

Water-stable Cu2S quantum dots were obtained by applying l-cysteine as a Cu(ii) to Cu(i) reducer and stabilizer in water and using an inert atmosphere at ambient temperature. The obtained quantum dots were characterized by STEM, XRD, FT-IR, UV-Vis, Raman, and fluorescence spectroscopy. The synthesis was optimized to achieve Cu2S quantum dots with an average diameter of about 9 nm that show red fluorescence emission. l-cysteine stabilization mediates crystallite growth, avoids aggregation of the quantum dots, and allows water solubility through polar functional groups, improving the fluorescence. The fluorometric test in the presence of the aptamer showed a shift in fluorescence intensity when an aliquot of As(iii) with a concentration of 100 pmol l-1 is incorporated because As(iii) and the used aptamer make a complex, leaving free the quantum dots and recovering their fluorescence response. The developed Cu2S quantum dots open possibilities for fluorescent detection of different analytes by simply changing aptamers according to the analyte to be detected.

Nanoparticle and Transparent Polymer Coatings Enable UV-C Side-Emission Optical Fibers for Inactivation of Escherichia coli in Water.

Pathogenic bacteria pose a health threat and operational challenge in drinking water. UV-C light-emitting diodes (UV-C LEDs) are becoming a competitive disinfection technology but are limited by their small irradiation area. Side-emitting optical fibers (SEOFs) can serve as a UV-C LED light delivery technology for reactors or tubing. Modifying the surfaces of conventional optical fibers with scattering centers allows for side emission of 265 nm radiation from an LED for microbial inactivation in water. Solid-material absorbance and flux measurements differentiated light absorption from scattering for all materials. Silica spheres >200 nm in diameter achieved higher scattering than smaller silica. A critical discovery was that treating the silica-coated optical fiber in a solution of high ionic strength increased UV-C side emission by greater than 6-fold. Additionally, the cladding polymer Cytop had negligible absorbance at 265 nm wavelength. A scalable four-step treatment process was developed to fabricate the novel SEOF. Attached to a 265 nm LED, the side-emitting optical fiber achieved 2.9 log inactivation of Escherichia coli at a delivery dose of 15 mJ/cm2. The results illustrate proof of concept that UV-C SEOFs can inactivate E. coli and should be further explored for delivering LED light into water.

Effects of abnormal temperature and starvation on the internal defense system of the schistosome-transmitting snail Biomphalaria glabrata.

Climate change may affect the internal defense system (IDS) of freshwater snails, and as a result their capacity to transmit disease. We examined effects of short-term exposure to supra- and sub-optimal temperatures or starvation on 3 parameters of the IDS of the schistosome-resistant Salvador strain of Biomphalaria glabrata - hemocyte concentrations, cell division in the amebocyte-producing organ (APO), and resistance to infection with Schistosoma mansoni. Adult snails were exposed to 1 of 3 temperatures, 20°C, 27°C (controls), or 33°C, for 1 or 2weeks, with food. A fourth group was maintained at 27°C, but without food. Compared to the controls, starved snails had significantly higher hemocyte counts at both 1 and 2weeks, although mitotic activity in the APO was significantly lower at both time periods. Exposure to 20°C or 33°C for 1 or 2weeks did not affect hemocyte numbers. However, APO mitotic activity in snails exposed to 20°C was significantly higher at both 1 and 2weeks, whereas mitotic activity in snails exposed to 33°C was significantly lower at 1week but normal at 2weeks. None of the treatments altered the resistance phenotype of Salvador snails. In a follow-up experiment, exposure to 33°C for 4-5h, a treatment previously reported to both induce expression of heat shock proteins (Hsps) and abrogate resistance to infection, caused immediate upregulation of Hsp 70 and Hsp 90 expression, but did not alter resistance, and Hsp expression levels returned to baseline after 2weeks at 33°C. Results of this study indicate that abnormal environmental conditions can have both stimulatory and inhibitory effects on the IDS in adult B. glabrata, and that some degree of acclimation to abnormal temperatures may occur.