ABSTRACT
Chagas disease (CD) is caused by the protozoan Trypanosoma cruzi, which leads to a spectrum of clinical presentations that range from asymptomatic to severe cardiac involvement. The host immune response plays a pivotal role in disease progression. Ig isotypes may contribute to disease pathogenesis. Investigating these components can provide insights into the immunopathogenic mechanisms underlying CD. This cross-sectional study aims to establish a correlation between the Ig profile of individuals infected with T. cruzi with the clinical forms of chronic CD. Serum samples were collected from partner institutions in different states of Brazil. Individuals diagnosed with chronic CD were categorized based on the clinical form of the disease. The indirect ELISA method using the recombinant chimeric Molecular Biology Institute of Paraná membrane protein 8.4 as the antigen was used to determine the Ig profile, including total IgG, IgG1, IgG2, IgG3, and IgG4. Ninety-seven serum samples from patients classified as negative (NEG, n = 38), indeterminate (IND, n = 24), mild cardiac (MC, n = 20), and severe cardiac (SC, n = 15) forms were analyzed. IgG1 exhibited greater levels compared with the other isotypes, showing a significant difference between the MC and IND groups. IgG3 levels were greater in individuals from the MC group compared with the SC group. IgG1 and IgG3 isotypes can serve as biomarkers to evaluate the progression of CD because they exhibit variations across clinical groups. Additional longitudinal studies are necessary to explore the relationship between antibody kinetics and the development of tissue damage.
Subject(s)
Chagas Disease , Trypanosoma cruzi , Humans , Trypanosoma cruzi/genetics , Recombinant Fusion Proteins , Cross-Sectional Studies , Antigens, Protozoan , Chagas Disease/diagnosis , Immunoglobulin G , Antibodies, ProtozoanABSTRACT
A dengue é atualmente um dos principais problemas de saúde pública do mundo e segundo a Organização Mundial de Saúde (OMS) é a doença que mais acomete o homem na atualidade. Sua incidência vem aumentando e estima-se que 50-100 milhões de pessoas desenvolvam a doença a cada ano no mundo. O diagnóstico laboratorial da dengue é realizado por diferentes tipos de testes, entre eles estão o isolamento viral, o RT-PCR, e a detecção por ELISA ou por meio de testes rápidos do antígeno viral NS1 e de anticorpos IgM específicos contra o vírus. A fim de contribuir para um melhor entendimento sobre a validade destes testes em diferentes circunstâncias, o objetivo principal deste trabalho foi avaliar a validade dos diferentes métodos laboratoriais no diagnóstico da dengue. A sensibilidade dos testes diagnósticos, ELISA IgM, ELISA NS1 e RT-PCR, foi avaliada de forma individual e de forma combinada utilizando amostras de soro de 623 pacientes incluídos em um estudo prospectivo de vigilância de base populacional entre fevereiro e julho de 2010...
Dengue is currently one of the main problems of public health and the world according to the World Health Organization (WHO) is the disease that affects more men today. Its incidence is increasing and it is estimated 50-100 million people develop the disease each year worldwide. Laboratory diagnosis of dengue is done by testing different types, which include viral isolation, RT-PCR, and detection by ELISA or by rapid viral tests NS1 antigen and specific IgM antibodies against the virus. In order to contribute to a better understanding of the validity of these tests in different circumstances, the aim of this study was to evaluate the validity of different laboratory methods for diagnosis of dengue. The sensitivity of diagnostic tests, IgM ELISA, ELISA NS1 and RT-PCR was evaluated individually and in combination form using serum samples from 623 patients enrolled in a prospective population-based study of surveillance between February and July 2010...
Subject(s)
Humans , Dengue Virus/immunology , Dengue Virus/isolation & purification , Dengue Virus/pathogenicityABSTRACT
Descrever o cuidado do enfermeiro ao individuo hospitalizado, que apresenta uma ferida traumática por esmagamento. Método: O método utilizado consiste em um relato de caso que contém informações organizadas sobre uma paciente vítima de acidente automobilístico. Resultados: Para análisedos dados foram definidos quatro momentos: primeiro momento (inflamatório), segundo momento(proliferativo), terceiro momento (cuidado com o enxerto) e quarto momento (remodelagem/maturação).Conclusão: O relato de caso revelou que o enfermeiro é imprescindível no cuidado de feridas e que o constante movimento dialógico com a equipe interdisciplinar é de suma importância para recuperação e reabilitação do paciente hospitalizado que possui uma lesão cutânea proveniente de um trauma.
Objective: To describe the care of the professional nurse with the hospitalized individual who has a traumatic wound by smashing. Method: The method consists of a case containing organized information about a patient from an automobile accident. Results: For data analysis, four stages were defined: the first stage (inflammatory), second stage (proliferative), third stage (caring with the graft) and the fourth stage(remodeling / maturation). Conclusion: The report of this case revealed that the nurse is essential in the caring of wounds and that the constant approach of dialogue with the interdisciplinary team is critical to the recovery and the rehabilitation of the hospitalized patient who has a skin lesion from a trauma.
Describir el cuidado de la enfermera de la persona en el hospital, que tiene una herida traumática por aplastamiento. Metodo: El método utilizado es um relato del caso que contiene la información sobre un paciente víctima del accidente automovilístico. Resultados: Para el análisis de datos ha definido cuatro etapas: la primera (inflamatoria), segunda etapa (proliferativa), terce (cuidado del injerto) y la cuarta (remodelación y maduración). Conclusión: Este caso revela que la enfermera es fundamental en el cuidado de las heridas y el movimiento constante de diálogo con el equipo interdisciplinario es fundamental en la recuperación y rehabilitación del paciente hospitalizado, que tiene una lesión en la piel de un trauma.
