[Comparative study on diagnostic probes: spirometry vs plethysmography in asymptomatic adult asthmatics]. / Estudio comparativo de dos pruebas diagnósticas: espirometría vs pletismografía en adultos asmáticos asintomáticos.

MATERIAL AND METHOD: The study to aim to do the comparison the sensibility between spirometry and pletismography in 20 asthmatic adult free of acute symptoms in basals conditions and to use subsequent of salbutamol spray. RESULTS: Were found to equal value for vital capacity (CV) with p = 0.02 for the both methods. For the total capacity pulmonary (CPT) with p = 0.05, p = 0.02 and increment in residual volume (VA) with p = 0.001 in the pletismography in both conditions (basal and post-salbutamol). The specific conductance (CE) to show p = < 0.001. The flows velocities (VF50, VF25-75) and (VF75, VF75-85) to show similar obstructions for aerial track smaller and smallest with p = < 0.20. CONCLUSION: Than both methods to have the same specificity, but no sensibility. The pletismography apparently to be more sensitive to diagnostic obstruction in airway tract of caliber medium.

In situ survival of plasmid-bearing and plasmidless Pseudomonas aeruginosa in pristine tropical waters.

Two rare wild-type strains of Pseudomonas aeruginosa were mixed in membrane diffusion chambers and then introduced into a natural freshwater environment for 72 h. The plasmid-containing strain (R serotype 15) and the plasmidless strain (H serotype 5) had initial bacterial densities of 2 x 10(5) cells per ml. Samples collected from the chambers were analyzed for viable and direct counts and for acquired-resistance frequencies. Suspected transconjugant-to-donor ratios ranged from 0.5 to 1.3; transfer percentages ranged from 13 to 70%. [3H]thymidine uptake indicated DNA synthesis in both strains as well as in transconjugants. These studies indicate that rare wild-type bacterial strains with large plasmid loads can survive as well as can bacteria with low plasmid loads when exposed to the in situ conditions of a tropical freshwater habitat. These results also suggest that genetic modification of indigenous microbiota through conjugation or transformation is feasible when rare wild-type strains or genetically engineered microorganisms are released in large numbers in tropical aquatic ecosystems.