[Jorge Lobo's disease. Second Peruvian case]. / Enfermedad de Jorge Lobo. Segundo caso Peruano.

A case of Jorge Lobo's disease is described. According to the references consulted the case presented is the second in Peru. The patient is a native of the Peruvian jungle (River Madre de Dios, State Madre de Dios). The disease was restricted to the left ear. Clinical and histopathological aspects were typical of Lobo's disease.

[Experimental infection of goats with Brucella ovis. Bacteriological serological and histopathological studies]. / Infección experimental de caprinos machos con Brucella ovis. Estudio bacteriológico, serológico e histopathologico.

The susceptibility of adult male goats to Brucella ovis infection was studied. Fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of B. ovis strain recently isolated from a case of ram epididymitis. Five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. B. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. The five goats sacrificed 78 days after inoculation contained Brucella in their organs. The semen and the tissues of the two other groups remained culturally negative throughout the observation period. Seven cultures were obtained from the semen of 14 rams used comparatively as inoculated controls. Epididymitis was clinically observed only in one male goat although under six presented macroscopic lesions. Seven rams out of the 15 inoculated showed clinical symptoms of epididymitis. Antibodies detectable by complement fixation and immunodiffusion disappeared 80 days after inoculation in goats, while rams reacted during the 189 days period of observation. It is concluded that the B. ovis infection in male goats is transient and the role that they may play in the epizootiology of the disease is negligible.

Infección experimental de caprinos machos con Brucella ovis. Estudio bacteriológico, serológico e histopathologico / [Experimental infection of goats with Brucella ovis. Bacteriological serological and histopathological studies]

The susceptibility of adult male goats to Brucella ovis infection was studied. Fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of B. ovis strain recently isolated from a case of ram epididymitis. Five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. B. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. The five goats sacrificed 78 days after inoculation contained Brucella in their organs. The semen and the tissues of the two other groups remained culturally negative throughout the observation period. Seven cultures were obtained from the semen of 14 rams used comparatively as inoculated controls. Epididymitis was clinically observed only in one male goat although under six presented macroscopic lesions. Seven rams out of the 15 inoculated showed clinical symptoms of epididymitis. Antibodies detectable by complement fixation and immunodiffusion disappeared 80 days after inoculation in goats, while rams reacted during the 189 days period of observation. It is concluded that the B. ovis infection in male goats is transient and the role that they may play in the epizootiology of the disease is negligible.

Infección experimental de caprinos machos con Brucella ovis. Estudio bacteriológico, serológico e histopathologico. / [Experimental infection of goats with Brucella ovis. Bacteriological serological and histopathological studies]

The susceptibility of adult male goats to Brucella ovis infection was studied. Fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of B. ovis strain recently isolated from a case of ram epididymitis. Five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. B. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. The five goats sacrificed 78 days after inoculation contained Brucella in their organs. The semen and the tissues of the two other groups remained culturally negative throughout the observation period. Seven cultures were obtained from the semen of 14 rams used comparatively as inoculated controls. Epididymitis was clinically observed only in one male goat although under six presented macroscopic lesions. Seven rams out of the 15 inoculated showed clinical symptoms of epididymitis. Antibodies detectable by complement fixation and immunodiffusion disappeared 80 days after inoculation in goats, while rams reacted during the 189 days period of observation. It is concluded that the B. ovis infection in male goats is transient and the role that they may play in the epizootiology of the disease is negligible.

Laboratory investigations on neuroparalytic accidents associated with suckling mouse brain rabies vaccine. III. -- Preservation of vaccine potency after elimination of murine brain myelin by centrifugation.

Myelin, which has been found in nine day-old mouse brain, was eliminated from weanling mouse brain suspensions by centrifugation at 17,000 g for 10 min, as demonstrated by electron microscopy and guinea-pig inoculation tests for encephalitogenic activity. This centrifugation procedure did not affect the potency of seven batches of suckling mouse brain vaccine, when centrifuged and non-centrifuged samples of the same batches were compared by a modified NIH potency test (t = 0.17). The present results with weanling mouse brain preparations suggest that centrifugation at 17,000 g be used in the preparation of rabies suckling mouse brain vaccine instead of the 1,900 g currently employed, which does not eliminate myelin. This new procedure would be expected to reduce the number of postvaccinal reactions which are attributable to the small amount of myelin which remains in vaccines prepared with new-born animal brains following the current procedure.

Some hematologic and temperature determinations in the 7-banded armadillo (Dasypus hybridus).

Hematologic values were determined for 10 male and 16 female 7-banded armadillos (Dasypus hybridus), known as the "mulita" in Argentina. Values for females and males, respectively, were: PCV 40.47 +/- 1.26 and 39.67 +/- 1.40 ml/dl; sedimentation rate 15.3 +/- 3.26 and 10.6 +/- mm/hr; RBC 6.001 +/- 0.128 and 5.963 +/- 0.170 X 10(6)/mm3; WBC 8.425 +/- 1.417 and 10.084 +/- 1.001 X 10(3)/mm3; hemoglobin 16.14 +/- 0.66 and 16.84 +/- 0.52; MCV, MCH, MCHC, and differential leukocyte count were also determined. An ultrastructural study of neutrophilic leukocytes and sex chromatin was made. Rectal temperatures ranged from 29.5-32 degrees C. These results were compared with findings for the 9-banded armadillo (Dasypus novemcinctus).