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1.
Syst Appl Microbiol ; 26(3): 453-65, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14529189

ABSTRACT

We have analysed 198 fast-growing soybean-nodulating rhizobial strains from four different regions of China for the following characteristics: generation time; number of plasmids; lipopolysaccharide (LPS), nodulation factors (LCOs) and PCR profiles; acidification of growth medium; capacity to grow at acid, neutral, and alkaline pH; growth on LC medium; growth at 28 and 37 degrees C; melanin production capacity; Congo red absorption and symbiotic characteristics. These unbiased analyses of a total subset of strains isolated from specific soybean-cropping areas (an approach which could be called "strainomics") can be used to answer various biological questions. We illustrate this by a comparison of the molecular characteristics of five strains with interesting symbiotic properties. From this comparison we conclude, for instance, that differences in the efficiency of nitrogen fixation or competitiveness for nodulation of these strains are not apparently related to differences in Nod factor structure.


Subject(s)
Glycine max/microbiology , Rhizobium/physiology , Symbiosis , Bacterial Proteins/analysis , China , Congo Red/metabolism , DNA Fingerprinting , DNA, Bacterial/isolation & purification , DNA, Ribosomal/analysis , DNA, Ribosomal Spacer/analysis , Electrophoresis, Agar Gel , Electrophoresis, Polyacrylamide Gel , Lipopolysaccharides/analysis , Melanins/biosynthesis , Plasmids , Polymorphism, Restriction Fragment Length , RNA, Ribosomal, 16S/genetics , Random Amplified Polymorphic DNA Technique , Rhizobium/chemistry , Rhizobium/genetics , Rhizobium/isolation & purification
2.
J Bacteriol ; 174(12): 4026-35, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1597418

ABSTRACT

A group of four rhi (rhizosphere-expressed) genes from the symbiotic plasmid of Rhizobium leguminosarum biovar viciae has been characterized. Although mutation of the rhi genes does not normally affect nodulation, in the absence of the closely linked nodulation genes nodFEL, mutations in the rhi genes can influence the nodulation of the vetch Vicia hirsuta. The DNA sequence of the rhi gene region reveals four large open reading frames, three of them constituting an operon (rhiABC) transcribed convergently toward the fourth gene, rhiR. rhiABC are under the positive control of RhiR, the expression of which is repressed by flavonoids that normally induce nod gene expression. This repression, which requires the nodD gene product (the transcriptional activator of nod gene expression), may be due to a cis effect caused by a high level of NodD-dependent expression from the adjacent nodO promoter, which is transcribed divergently from rhiR. RhiR shows significant similarities to a subfamily of transcriptional regulators that includes the LuxR and UvrC-28K proteins. RhiA shows limited homology to a short domain of the lactose permease, LacY, close to a region thought to be involved in substrate binding. No strong homologies were found for the other rhi gene products. It appears that RhiA and RhiB are cytoplasmic, whereas RhiC is a periplasmic protein, since it has a typical N-terminal transit sequence and a rhiC-phoA protein fusion expresses alkaline phosphatase activity. The biochemical role of the rhi genes has not been established, but it appears that they may play a role in the plant-microbe interaction, possibly by allowing the bacteria to metabolize a plant-made metabolite.


Subject(s)
Bacterial Proteins/genetics , DNA-Binding Proteins , Gene Expression Regulation, Bacterial/genetics , Hesperidin , Operon/genetics , Rhizobium leguminosarum/genetics , Trans-Activators , Amino Acid Sequence , Bacterial Proteins/chemistry , Base Sequence , Flavonoids/pharmacology , Gene Expression Regulation, Bacterial/drug effects , Molecular Sequence Data , Plasmids/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Sequence Alignment
3.
Appl Environ Microbiol ; 54(7): 1812-7, 1988 Jul.
Article in English | MEDLINE | ID: mdl-16347690

ABSTRACT

Different Rhizobium and Bradyrhizobium strains were screened for their ability to produce melanin. Pigment producers (Mel) were found among strains of R. leguminosarum biovars viceae, trifolii, and phaseoli, R. meliloti, and R. fredii; none of 19 Bradyrhizobium strains examined gave a positive response. Melanin production and nod genes were plasmid borne in R. leguminosarum biovar trifolii RS24. In R. leguminosarum biovar phaseoli CFN42 and R. meliloti GR015, mel genes were located in the respective symbiotic plasmids. In R. fredii USDA 205, melanin production correlated with the presence of its smallest indigenous plasmid.

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