ABSTRACT
MicroRNAs (miRNAs) play critical roles in many different cellular processes, including metabolism, apoptosis, differentiation, and development. In this study, miR-663 was shown to be highly expressed in patients with lung cancer. Furthermore, miR-663 contributed to lung cancer cell proliferation of by regulating TGFB1, P53, Bax, and Fas directly or indirectly. Our results demonstrated that miR-663 plays an important role in the biology of lung cancer and may be useful in developing therapies targeting genes.
Subject(s)
Lung Neoplasms/genetics , Lung Neoplasms/metabolism , MicroRNAs/metabolism , Transforming Growth Factor beta1/metabolism , Apoptosis/genetics , Cell Line, Tumor , Cell Proliferation , Fas Ligand Protein/metabolism , Gene Expression Regulation, Neoplastic , Humans , MicroRNAs/genetics , Tumor Suppressor Protein p53/metabolism , bcl-2-Associated X Protein/metabolismABSTRACT
AIM: To study the influence of astragalus and zinc sulfate on the viscosity in erythrocyte membrane during intestinal I/R and their mechanism of action. METHODS: Models of rabbits intestinal I/R injury were made. The effect of astragalus and zinc sulfate on the viscosity and malondialdehyde (MDA) in erythrocyte membrane, superoxide dismutase (SOD) in erythrocyte, oxidase (XO) in plasma and MDA tissues homogenate were observed. RESULTS: The administration of astragalus and zinc sulfate decreased viscosity and MDA and XO, prevented the reduction of SOD, and alleviated I/R injury. CONCLUSION: Lipid peroxidation injury of the erythrocyte membrane was one of the pathogenesis of I/R injury, and astragalus and the zinc sulfate possessed effects of anti-lipid peroxide, stabilized erythrocyte membrane, increased red blood cell deform ability and raised microcircular perfusion.
