Ectopic expression of FvWRKY42, a WRKY transcription factor from the diploid woodland strawberry (Fragaria vesca), enhances resistance to powdery mildew, improves osmotic stress resistance, and increases abscisic acid sensitivity in Arabidopsis.

WRKY transcription factors play a critical role in biotic and abiotic stress responses in plants, but very few WRKYs have been reported in strawberry plants. Here, a multiple stress-inducible gene, FvWRKY42, was isolated from the wild diploid woodland strawberry (accession Heilongjiang-3). FvWRKY42 expression was induced by treatment with powdery mildew, salt, drought, salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), and ethylene. The protein interaction network analysis showed that the FvWRKY42 protein interacts with various stress-related proteins. Overexpression of FvWRKY42 in Arabidopsis resulted in cell death, sporulation, slow hypha growth, and enhanced resistance to powdery mildew that was concomitant with increased expression of PR1 genes in Arabidopsis. Overexpression also led to enhanced salt and drought stress tolerance, increased primary root length and germination rate, decreased water loss rate, reduced relative electrolyte leakage, and malondialdehyde accumulation, and upregulation of superoxide dismutase and catalase activity. Additionally, FvWRKY42-overexpressing Arabidopsis plants showed increased ABA sensitivity during seed germination and seedling growth, increased stomatal closure after ABA and drought treatment, and altered expression of ABA-responsive genes. Collectively, our data demonstrate that FvWRKY42 may play an important role in powdery mildew infection and the regulation of salt and drought stress responses in plants.

Identification and Transcript Analysis of the TCP Transcription Factors in the Diploid Woodland Strawberry Fragaria vesca.

Plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors play versatile functions in multiple processes of plant growth and development. However, no systematic study has been performed in strawberry. In this study, 19 FvTCP genes were identified in the diploid woodland strawberry (Fragaria vesca) accession Heilongjiang-3. Phylogenetic analysis suggested that the FvTCP genes were classified into two main classes, with the second class further divided into two subclasses, which was supported by the exon-intron organizations and the conserved motif structures. Promoter analysis revealed various cis-acting elements related to growth and development, hormone and/or stress responses. We analyzed FvTCP gene transcript accumulation patterns in different tissues and fruit developmental stages. Among them, 12 FvTCP genes exhibited distinct tissue-specific transcript accumulation patterns. Eleven FvTCP genes were down-regulated in different fruit developmental stages, while five FvTCP genes were up-regulated. Transcripts of FvTCP genes also varied with different subcultural propagation periods and were induced by hormone treatments and biotic and abiotic stresses. Subcellular localization analysis showed that six FvTCP-GFP fusion proteins showed distinct localizations in Arabidopsis mesophyll protoplasts. Notably, transient over-expression of FvTCP9 in strawberry fruits dramatically affected the expression of a series of genes implicated in fruit development and ripening. Taken together, the present study may provide the basis for functional studies to reveal the role of this gene family in strawberry growth and development.