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1.
J Anim Sci ; 95(3): 1232-1242, 2017 Mar.
Article in English | MEDLINE | ID: mdl-28380514

ABSTRACT

The present study investigated the effects of different feeding levels during 3 short periods of gestation on gilt and litter performance, apparent total tract digestibility (ATTD) of energy and nutrients, and energy homeostasis in gilts. A total of 18 gilts were allotted to 1 of 3 dietary treatments using a completely randomized design. All gilts were fed 1 common corn-soybean meal-based diet with the amount of 1.0 × maintenance energy intake (100 × BW (BW) kcal ME/d) throughout gestation except 3 periods of 7 d when dietary treatments were imposed on d 27, d 55, and d 83 of gestation. During the 3 short periods, gilts were fed 1 of 3 different feeding levels: 0.5, 1.0, and 2.0 × maintenance energy level (0.5M, 1.0M, and 2.0M, respectively). Results showed that gilts on 2.0M feeding level had higher ( < 0.05) weight gain from d 27 to 109 of gestation (37.05 vs. 15.34 kg) and greater ( < 0.05) BW change, average daily gain, and gain to feed ratio during gestation periods 1 (d 27-34) and 3 (d 83-90) when compared with gilts on 0.5M feeding level. No differences ( > 0.10) in litter performance were observed among the 3 feeding levels. Additionally, the slopes of BW change in response to feeding levels in period 1 were 4.32 kg/0.5M change from 0.5M to 1.0M feeding level and 3.72 kg/0.5M change from 1.0M to 2.0M feeding level, respectively. There were quadratic ( < 0.05) effects of feeding levels on ATTD of dry matter and gross energy during periods 1 and 2 (d 55-62). Furthermore, fasting plasma concentrations of acyl ghrelin and nonesterified fatty acid (NEFA) in period 1 were greater ( < 0.01) in gilts on 0.5M feeding level than those on 2.0M feeding level. In conclusion, increasing feeding levels during 3 short periods increased primiparous sow performance during these short periods but did not affect litter performance. ATTD of energy and nutrients, and BW change efficiency were maximized for gilts on 1.0 M feeding level. The data also indicated that sows on the lowest feeding level were exposed to negative energy balance as evidenced by the higher plasma acyl ghrelin and NEFA concentrations.


Subject(s)
Energy Metabolism , Swine/physiology , Animals , Diet/veterinary , Eating , Energy Intake , Female , Homeostasis , Lactation , Parity , Pregnancy , Glycine max , Weight Gain
2.
Braz. j. med. biol. res ; 48(6): 515-522, 06/2015. graf
Article in English | LILACS | ID: lil-748224

ABSTRACT

We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.


Subject(s)
Animals , Male , Ethanol/poisoning , Isoflavones/therapeutic use , Maze Learning/drug effects , Memory Disorders/prevention & control , Neuroprotective Agents/therapeutic use , Spatial Memory/drug effects , Vasodilator Agents/therapeutic use , Alcoholism/complications , Chromatography, High Pressure Liquid , Cerebral Cortex/chemistry , Cerebral Cortex/drug effects , Enzyme-Linked Immunosorbent Assay , Glutamic Acid/analysis , Interleukin-1beta/analysis , Isoflavones/pharmacology , Memory Disorders/chemically induced , Memory Disorders/drug therapy , Microglia/drug effects , Neuroprotective Agents/pharmacology , Random Allocation , Time Factors , Treatment Outcome , Tumor Necrosis Factor-alpha/analysis , Vasodilator Agents/pharmacology , gamma-Aminobutyric Acid/analysis
3.
Braz J Med Biol Res ; 48(6): 515-22, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25831201

ABSTRACT

We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1ß were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1ß increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.


Subject(s)
Ethanol/poisoning , Isoflavones/therapeutic use , Maze Learning/drug effects , Memory Disorders/prevention & control , Neuroprotective Agents/therapeutic use , Spatial Memory/drug effects , Vasodilator Agents/therapeutic use , Alcoholism/complications , Animals , Cerebral Cortex/chemistry , Cerebral Cortex/drug effects , Chromatography, High Pressure Liquid , Enzyme-Linked Immunosorbent Assay , Glutamic Acid/analysis , Interleukin-1beta/analysis , Isoflavones/pharmacology , Male , Memory Disorders/chemically induced , Memory Disorders/drug therapy , Mice, Inbred C57BL , Microglia/drug effects , Neuroprotective Agents/pharmacology , Random Allocation , Time Factors , Treatment Outcome , Tumor Necrosis Factor-alpha/analysis , Vasodilator Agents/pharmacology , gamma-Aminobutyric Acid/analysis
4.
Genet Mol Res ; 12(2): 1720-30, 2013 May 21.
Article in English | MEDLINE | ID: mdl-23765978

ABSTRACT

We made a Human Genome Epidemiology review and meta-analysis to examine a possible association between interleukin-1 receptor antagonist (IL-1RN) polymorphisms and susceptibility to ankylosing spondylitis (AS). Studies of IL-1RN polymorphisms and susceptibility to AS were found by searching the Pubmed, Cochrane library, Embase, Web of Science, Springerlink, CNKI, and CBM databases. Data were extracted by 2 independent reviewers. The meta-analysis was performed with the Review Manager Version 5.1.6 and STATA Version 12.0 software. The odds ratio (OR) and 95% confidence intervals (95%CI) were calculated based on the extracted data. Thirteen studies with 5391 AS cases and 5239 healthy controls were retrieved. Seven IL-1RN polymorphisms were addressed, including rs30735, rs31017, rs419598, rs315951, rs315952, rs27810, and VNTR. Meta-analysis showed that the rs30735*C allele/carrier, the rs31017*G carrier and the rs315952*T carrier were positively and significantly associated with susceptibility to AS (OR = 1.45, 95%CI = 1.19-1.76; OR = 1.73, 95%CI = 1.34-2.24; OR = 1.30, 95%CI = 1.01-1.69; OR = 1.54, 95%CI = 1.16-2.04). A subgroup analysis based on ethnicity revealed significant positive associations between the rs30735*C allele/carrier and the rs31017*G allele and susceptibility to AS in both Caucasian and Asian populations, while the positive association between the rs315952*T carrier and AS susceptibility was significant only in Asian populations (OR = 1.54, 95%CI = 1.16-2.04). This meta-analysis suggests that IL-1RN polymorphisms are involved in the pathogenesis of AS. The rs30735*C allele/carrier, and the rs31017*G allele may be risk factors for ankylosing spondylitis in Caucasians and Asians, while the rs315952*T carrier is associated with susceptibility to this disease only in Asians.


Subject(s)
Genetic Association Studies , Genetic Predisposition to Disease , Genome, Human/genetics , Interleukin 1 Receptor Antagonist Protein/genetics , Polymorphism, Single Nucleotide/genetics , Spondylitis, Ankylosing/genetics , Alleles , Humans , Linear Models , Publication Bias , Risk Factors
5.
Environ Res ; 80(2 Pt 1): 127-31, 1999 Feb.
Article in English | MEDLINE | ID: mdl-10092404

ABSTRACT

Genotoxic activity appears to originate primarily from reactions of chlorine with humic substances in the source waters. Comparisons of extracts of settled versus chlorinated water have confirmed that chlorinating during water treatment produces mutagenic activity in the mutagenicity tests. Present work on XAD-2 extracts of raw, chlorinated (treated), and settled water from the Chao Lake region of China has involved a battery of mutagenicity assays for various genetic endpoints: the Salmonella test, the sister-chromatid exchange (SCE) induction in Chinese hamster lung (CHL) cells, and the micronucleus (MN) induction in the peripheral blood erythrocytes of silver carp. Extracts of raw and treated water but not the settled water are mutagenic in the Salmonella assay. On the other hand, extracts of three water samples show activity in the SCE and MN assays, especially the raw and treated water. These data show that contamination and chlorinating contribute mutagens to drinking water and suggest that the mammalian assays may be more sensitive for detecting mutagenicity in aquatic environment than the Salmonella test.


Subject(s)
Chlorine/toxicity , Point Mutation , Sister Chromatid Exchange , Water Pollution, Chemical/adverse effects , Water Supply/analysis , Animals , CHO Cells/drug effects , Carps , Cricetinae , Erythrocytes/drug effects , Micronucleus Tests , Mutagenicity Tests , Salmonella/drug effects , Salmonella/genetics , Sensitivity and Specificity
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