ABSTRACT
Due to its role in apoptosis, differentiation, cell cycle arrest, and DNA damage repair in stress responses (oxidative stress, hypoxia, chemotherapeutic drugs, and UV irradiation or radiotherapy), FOXO3a is considered a key tumor suppressor that determines radiotherapeutic and chemotherapeutic responses in cancer cells. Mutations in the FOXO3a gene are rare, even in cancer cells. Post-translational regulations are the main mechanisms for inactivating FOXO3a. The subcellular localization, stability, transcriptional activity, and DNA binding affinity for FOXO3a can be modulated via various post-translational modifications, including phosphorylation, acetylation, and interactions with other transcriptional factors or regulators. This review summarizes how proteins that interact with FOXO3a engage in cancer progression.
Subject(s)
Forkhead Box Protein O3 , Neoplasms , Humans , Acetylation , Apoptosis , Cell Differentiation , Neoplasms/genetics , Transcription Factors , Forkhead Box Protein O3/geneticsABSTRACT
As a renewable resource, the market trend of plant protein has increased significantly in recent years. Compared with animal protein, plant protein production has strong sustainability factors and a lower environmental impact. Many bioactive substances have poor stability, and poor absorption effects limit their application in food. Plant protein-based carriers could improve the water solubility, stability, and bioavailability of bioactive substances by different types of delivery systems. In this review, we present a detailed and concise summary of the effects and advantages of various plant protein-based carriers in the encapsulation, protection, and delivery of bioactive substances. Furthermore, the research progress of food-grade bioactive ingredient delivery systems based on plant protein preparation in recent years is summarized, and some current challenges and future research priorities are highlighted. There are some key findings and conclusions: (i) plant proteins have numerous functions: as carriers for transportation systems, a shell or core of a system, or food ingredients; (ii) plant protein-based carriers could improve the water solubility, stability, and bioavailability of bioactive substances by different types of delivery systems; and (iii) plant protein-based carriers stabilize bioactive substances with potential applications in the food and nutrition fields.
ABSTRACT
The design of gelatin-based hydrogels with high mechanical strength, high gelation temperature, and a rapid self-healing property still presents a challenge to researchers. In the present study, single cross-linked gelatin-oxidized tannic acid (SC-GT/OTA) hydrogels were fabricated through covalent cross-linking between gelatin and tannic acid (TA) oxidized by using sodium periodate (NaIO4). Double cross-linked gelatin-OTA-FeCl3·6H2O (DC-GT/OTA/FeIII) hydrogels were also created using metal coordination bonds formed between the catechol groups present in OTA and FeIII in ferric chloride. As a result, the maximum gelling temperature of the SC-GT/OTA hydrogel (37 °C) was considerably higher than that of the pure gelatin hydrogel (15.4 °C). Moreover, the maximum values of compressive stress of SC-GT/OTA hydrogels increased significantly by almost seven times the original value as the molar ratio of NaIO4 to TA increased from 3:1 to 5:1. When the molar ratio of NaIO4 to TA was maintained at the constant of 4:1, the storage modulus values of DC-GT/OTA/FeIII hydrogels with the FeIII-to-TA molar ratio of 1.5:1 were three to 4 orders of magnitude higher than those of SC-GT/OTA hydrogels in the whole angular frequency range. The double cross-linked gelatin hydrogels developed in this research can be used widely in agriculture and material science fields.
