Dynamical characterization and multiple unbinding paths of two PreQ1 ligands in one pocket.

Riboswitches naturally regulate gene expression in bacteria by binding to specific small molecules. Class 1 preQ1 riboswitch aptamer is an important model not only for RNA folding but also as a target for designing small molecule antibiotics due to its well-known minimal aptamer domain. Here, we ran a total of 62.4 µs conventional and enhanced-sampling molecular dynamics (MD) simulations to characterize the determinants underlying the binding of the preQ1-II riboswitch aptamer to two preQ1 ligands in one binding pocket. Decomposition of binding free energy suggested that preQ1 ligands at α and ß sites interact with four nucleotides (G5, C17, C18, and A30) and two nucleotides (A12 and C31), respectively. Mg2+ ions play a crucial role in both stabilizing the binding pocket and facilitating ligand binding. The flexible preQ1 ligand at the ß site leads to the top of the binding pocket loosening and thus pre-organizes the riboswitch for ligand entry. Enhanced sampling simulations further revealed that the preQ1 ligand at the α site unbinds through two orthogonal pathways, which are dependent on whether or not a ß site preQ1 ligand is present. One of the two preQ1 ligands has been identified in the binding pocket, which will aid to identify the second preQ1 Ligand. Our work provides new information for designing robust ligands.

Evaluation of future wetland changes under optimal scenarios and land degradation neutrality analysis in the Guangdong-Hong Kong-Macao Greater Bay Area.

Wetlands are one of the most productive ecosystems on Earth and are also focused on by the Sustainable Development Goals (SDGs). However, global wetlands have suffered from considerable degradation due to rapid urbanization and climate change. To support wetland protection and SDG reporting, we predicted future wetland changes and assessed land degradation neutrality (LDN) from 2020 to 2035 under four scenarios in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA). A simulation model combining random forest (RF), CLUE-S and multi-objective programming (MOP) methods was developed to predict wetland patterns under the natural increase scenario (NIS), economic development scenario (EDS), ecological protection and restoration scenario (ERPS) and harmonious development scenario (HDS). The simulation results indicated that the integration of RF and CLUE-S achieved good simulation accuracy, with OA over 0.86 and kappa indices over 0.79. From 2020 to 2035, the mangrove, tidal flat and agricultural pond increased while the coastal shallow water decreased under all scenarios. The river decreased under NIS and EDS, while increased under ERPS and HDS. The Reservoir decreased under NIS, while increased under the remaining scenarios. Among scenarios, the EDS had the largest built-up land and agricultural pond, and the ERPS had the largest forest and grassland. The HDS was a coordinated scenario that balanced economic development and ecological protection. Its natural wetlands were almost equal to these of ERPS, and its built-up land and cropland were almost equal to these of EDS. Then, the land degradation and SDG 15.3.1 indicators were calculated to support the LDN target. From 2020 to 2035, the ERPS had a smallest gap of 705.51 km2 from the LDN target, following the HDS, EDS and NIS. The SDG 15.3.1 indicator was lowest under the ERPS, with a value of 0.85 %. Our study could offer strong support for urban sustainable development and SDGs reporting.

Warming of surface water in the large and shallow lakes across the Yangtze River Basin, China, and its driver analysis.

A variety of physical, chemical, and biological processes within the lakes relies on the surface water temperature while the spatial pattern of large lakes of different warming trends and their connections with climate change remain unclear. Using correlation analysis, regression tree analysis (RTA), and general linear models (GLMs), we have estimated the warming trends of 192 lakes since 2000 in the populated Yangtze River Basin, China, to identify dominant climate drivers and quantify their contributions. The results show that surface water temperature has increased substantially in the majority of the investigated lakes (179 from a total of 192 lakes) at a rate of 0.29 (- 0.12 to 0.62) °C/decade (median and 95% confidence interval). The shallower lakes (< 13.1 m in depth) usually have the faster median warming rates than the deeper lakes (i.e., 0.37 °C/decade versus 0.16 °C/decade). We find that in the shallow lakes, rising air temperatures and declining wind speeds can explain the majority of variation in surface water temperature (i.e., 31.4‒80.3% and 13.0‒21.0%, respectively). In contrast, in deeper lakes, change of air temperatures plays a dominant role in water warming (75.4‒91.2%). This study has emphasized the importance of declining wind speed in water warming in large and shallow lakes and illustrated a difference of dominant climatic drivers in water warming between the shallow and deep lakes.

Smart Metasurface for Active and Passive Cooperative Manipulation of Electromagnetic Waves.

Integrating active and passive manipulation of electromagnetic (EM) waves has significant advantages for the caliber synthesis of microwave and optical integrated devices. In previous schemes, most reported designs focus only on active ways of manipulating self-radiating EM waves, such as antennas and lasers, or passive ways of manipulating external incident EM waves, such as lenses and photonic crystals. Here, we proposed a paradigm that integrates active and passive manipulation of EM waves in a reconfigurable way. As demonstrated, circularly polarized, linearly polarized, and elliptically polarized waves with customized beams are achieved in passive operation by merging Pancharatnam-Berry phases and dynamic phases, while the radiating EM waves with a customized gain are achieved by coupling the coding elements with the radiation structure in the active manipulation. Either active or passive manipulation is determined by the sensed signals and operating state to reduce detectability. Encouragingly, the proposed strategy will excite new sensing and communication opportunities, enabling advanced conceptions for next-generation compact EM devices.

Metasurface-based broadband polarization-insensitive polarization rotator.

The polarizations of electromagnetic (EM) waves are very important for transceivers. We propose a broadband polarization-insensitive polarization rotator (PIPR), which can realize 90° polarization rotation for incident waves with an arbitrary azimuth angle. A unit of the PIPR is composed of two types of substrate integrated waveguide (SIW) units in a checkerboard pattern, which provides more than -0.2 dB transmission from 9.5 to 10.9 GHz. The electric field inside the cavity is analyzed to explain the working mechanism of the proposed rotator. A prototype is fabricated and measured to verify the proposed design, and satisfactory agreement between simulated and measured results is achieved, indicating that the converter has potential applications in imaging and communication systems.

LncRNA FGD5-AS1 reduces cardiomyocyte apoptosis and inflammation by modulating Akt and miR-223-3p expression.

OBJECTIVES: Long non-coding RNAs (lncRNAs) are known to be involved in heart development and function. In this study, we aimed to explore the effect of the lncRNA FGD5 antisense RNA 1 (FGD5-AS1) on acute myocardial infarction (AMI) by targeting miR-223-3p. METHODS: An AMI model was established both in vivo and in vitro. The levels of FGD5-AS1, miR-223-3p and inflammatory factors were detected by real-time quantitative reverse transcription PCR. Cardiomyocyte apoptosis was assessed using TdT-mediated dUTP nick-end labeling assay. The protein levels of cleaved caspase-3, Bcl-2 and Bax were examined using Western blot. Cardiac function was evaluated using hemodynamic analysis and hematoxylin-eosin and Masson's trichrome staining. In addition, an underlying competitive endogenous RNA mechanism was revealed by bioinformatics analysis, dual-luciferase reporter assay and rescue experiments. RESULTS: We found decreased expression of FGD5-AS1 in AMI. Furthermore, FGD5-AS1 expression significantly decreased the infarct size, improved cardiac performance and attenuated cardiac fibrosis by reducing myocardial apoptosis and inflammation. miR-223-3p was a direct target of FGD5-AS1. Moreover, miRNA-223-3p directly downregulated the expression of phosphorylated Akt in primary neonatal rat cardiomyocytes. Further experiments demonstrated that FGD5-AS1 modulated Akt activity to reduce myocardial injury through miR-223-3p. CONCLUSION: The FGD5-AS1/miR-223-3p/Akt pathway is involved in AMI, suggesting that FGD5-AS1 may act as a potential biomarker and therapeutic target for AMI.

Chirality Enhancement Using Fabry-Pérot-Like Cavity.

Chiral molecules that do not superimpose on their mirror images are the foundation of all life forms on earth. Chiral molecules exhibit chiroptical responses, i.e., they have different electromagnetic responses to light of different circular polarizations. However, chiroptical responses in natural materials, such as circular dichroism and optical rotation dispersion, are intrinsically small because the size of a chiral molecule is significantly shorter than the wavelength of electromagnetic wave. Conventional technology for enhancing chiroptical signal entails demanding requirements on precise alignment of the chiral molecules to certain nanostructures, which however only leads to a limited performance. Herein, we show a new approach towards enhancement of chiroptical effects through a Fabry-Pérot (FP) cavity formed by two handedness-preserving metamirrors operating in the GHz region. We experimentally show that the FP cavity resonator can enhance the optical activity of the chiral molecule by an order of magnitude. Our approach may pave the way towards state-of-the-art chiral sensing applications.

Infrared-controlled programmable metasurface.

Programmable metasurface enables controlling electromagnetic (EM) waves in real time. By programming the states of active device embedded in metasurface element, the EM properties of the digital metasurface can be changed quickly without redesigning their structures. However, large numbers of long-distance wires are required to connect the programmable metasurface to provide the coded signals from field programmable gate array (FPGA) when controlling the metasurface at a long distance, which is complicated and inconvenient. Here, we propose an infrared-controlled programmable metasurface that can be programmed remotely. The infrared transceiver is able to switch the coding sequences stored in the FPGA controller, thus controlling the voltage on the varactors integrated in the metasurface. Experiment is performed at microwave frequencies, and the measured results verify that the scattering beams of the metasurface sample can be changed remotely by using infrared ray. The proposed infrared-controlled programmable metasurface opens up avenues for constructing a new class of remotely-tuning dynamic metasurfaces.

Deep Learning: A Rapid and Efficient Route to Automatic Metasurface Design.

Metasurfaces provide unprecedented routes to manipulations on electromagnetic waves, which can realize many exotic functionalities. Despite the rapid development of metasurfaces in recent years, the design process of metasurface is still time-consuming and computational resource-consuming. Moreover, it is quite complicated for layman users to design metasurfaces as plenty of specialized knowledge is required. In this work, a metasurface design method named REACTIVE is proposed on the basis of deep learning, as deep learning method has shown its natural advantages and superiorities in mining undefined rules automatically in many fields. REACTIVE is capable of calculating metasurface structure directly through a given design target; meanwhile, it also shows the advantage in making the design process automatic, more efficient, less time-consuming, and less computational resource-consuming. Besides, it asks for less professional knowledge, so that engineers are required only to pay attention to the design target. Herein, a triple-band absorber is designed using the REACTIVE method, where a deep learning model computes the metasurface structure automatically through inputting the desired absorption rate. The whole design process is achieved 200 times faster than the conventional one, which convincingly demonstrates the superiority of this design method. REACTIVE is an effective design tool for designers, especially for laymen users and engineers.

Asymmetric transmission of acoustic waves in a waveguide via gradient index metamaterials.

We demonstrate that asymmetric acoustic wave transmission in a waveguide can be achieved via gradient index metamaterials (GIMs). We theoretically prove that the acoustic wave can be efficiently converted to surface waves (SWs) via GIMs. The GIMs in a waveguide can allow the transmission of acoustic waves in one direction but block them in the other direction. This theory is validated by experiments. Our findings may provide new applications in various scenarios such as high-efficiency acoustic couplers and noise control.

Integrated spoof plasmonic circuits.

Using a metamaterial consisting of metals with subwavelength surface patterning, one can mimic surface plasmon polaritons (SPPs) and achieve surface waves with subwavelength confinement at microwave and terahertz frequencies, thus bringing most of the advantages associated with the optical SPPs to lower frequencies. Due to the properties of strong field confinement and high local field intensity, spoof SPPs have demonstrated the improved performance for data transmission and device miniaturization in an intensively integrated environment. The distinctive abilities, such as suppression of transmission loss and bending loss, and increase of signal integrity, make spoof SPPs a promising candidate for future generation of electronic circuits and electromagnetic systems. This article reviews the progress in spoof SPPs with a special focus on their applications in circuits from transmission lines to passive and active devices in microwave and terahertz regimes. The integration of versatile spoof SPP devices on a single platform, which is compatible with established electronic circuits, is also discussed.

Transparent coupled membrane metamaterials with simultaneous microwave absorption and sound reduction.

Metamaterials offer a novel strategy to control wave propagation in different physical fields ranging from acoustic, electromagnetic, and optical waves to static electric and thermal fields. However, fundamental and practical challenges still need to be overcome for multi-physical manipulation, especially for independent control of acoustic and electromagnetic waves simultaneously. In this paper, we propose and experimentally demonstrate a transparent bifunctional metamaterial in which acoustic and electromagnetic waves could be engineered jointly and individually. Specifically, a transparent composite coupled membrane metamaterial is introduced with indium tin oxide (ITO) patterns coated on the top and bottom membranes, giving rise to simultaneous electromagnetic wave dissipation and sound reduction. Our results could help broaden the current research scope for multiple disciplines and pave the way for the development of multi-functional devices in new applications.

Tailoring polarization states of multiple beams that carry different topological charges of orbital angular momentums.

In the past few years, orbital angular momentum (OAM) has aroused great interest in the scientific communities, because it shows great potential for enhancing capacities of radio and optical communication systems. Here, we propose anisotropic metasurfaces to generate multiple OAM vortex beams at microwave frequencies. A phase compensation theory is presented, in order to determine the phase distributions on metasurfaces, This theory enables independent control of beam numbers, polarizations, orientations, and topological charges of OAM vortex beams, respectively. The metasurface is composed of anisotropic elements, whose reflection phases can be engineered separately in different polarization directions. The scheme is validated by both simulation and experimental results and shows great potential for the polarization division multiplexing in OAM communication systems.

Extraordinary optical transmission through a rectangular hole filled with extreme uniaxial metamaterials.

This Letter presents a theory of extraordinary optical transmission (EOT) through a rectangular hole filled with the extreme uniaxial metamaterials with infinite longitudinal components of permittivity (ϵz) and permeability (µz). We demonstrate theoretically and numerically that a number of high-order transverse electromagnetic (TEM) modes can be supported by such a structure, and that, more interestingly, their normalized transmittance can be remarkably enhanced due to the Fabry-Perot resonance effect. A set of illustrative examples has been provided to demonstrate that such an EOT property could be explored for the purpose of subwavelength-resolution imaging.

Information entropy of coding metasurface.

Because of their exceptional capability to tailor the effective medium parameters, metamaterials have been widely used to control electromagnetic waves, which has led to the observation of many interesting phenomena, for example, negative refraction, invisibility cloaking, and anomalous reflections and transmissions. However, the studies of metamaterials or metasurfaces are mainly limited to their physical features; currently, there is a lack of viewpoints on metamaterials and metasurfaces from the information perspective. Here we propose to measure the information of a coding metasurface using Shannon entropy. We establish an analytical connection between the coding pattern of an arbitrary coding metasurface and its far-field pattern. We introduce geometrical entropy to describe the information of the coding pattern (or coding sequence) and physical entropy to describe the information of the far-field pattern of the metasurface. The coding metasurface is demonstrated to enhance the information in transmitting messages, and the amount of enhanced information can be manipulated by designing the coding pattern with different information entropies. The proposed concepts and entropy control method will be helpful in new information systems (for example, communication, radar and imaging) that are based on the coding metasurfaces.

Broadband, wide-angle, low-scattering terahertz wave by a flexible 2-bit coding metasurface.

Expanding bandwidths and arbitrary control of technology remain key issues in the field of electromagnetic waves, especially in terahertz (THz) wave. In this paper, we propose a novel method to achieve broadband low-scattering THz characteristics with wide-angle and polarization independence by a 2-bit flexible and nonabsorptive coding metasurface. The coding metasurface is composed of four digital elements based on double cross metallic line for "00", "01", "10", and "11." The reflection phase difference of neighboring elements is about 90° over a broad THz frequency band and wide incident angles. The low scattering coefficients below -10 dB were achieved over a wide frequency band from 0.8 THz to 1.5 THz when the incident angle is less than 50° by coding the four elements sequences. This superior property is maintained when the flexible coding metasurface is wrapped around a metallic cylinder with different dimensions. These results present a novel method to control THz waves freely and demonstrate significant scientific value in practical applications.

Macroscopic broadband optical escalator with force-loaded transformation optics.

Transformation optics enables one to guide and control light at will using metamaterials. However the designed device is deterministic and not flexible for different objects. Based on force-loaded transformation optics we propose a force-induced transformational device, which can realize dynamic escalator metamorphosing continuously between optical elevator and invisibility cloak. This escalator can visually lift up and down the perceived height of a plane fixed in space by controlling the forces loaded in different directions. Or conversely, the escalator can physically lift up and down a plane while visually maintaining the same height to an outside observer. One can quickly adjust this device to the required demand without changing the background index, while the usual transformation cloak will be detectable due to the lateral shift from mismatched background. The schematic is self-adaptive, multi-functional, and free of metamaterial or nanofabrication. Our work opens a new perspective in controlling light dynamically and continuously, empowering unprecedented applications in military cloak, optic communication, holographic imaging, and phase-involved microtechnique.

Controlled assembly of highly Raman-enhancing silver nanocap arrays templated by porous anodic alumina membranes.

A convenient nanoscale technique is reported for the fabrication of highly ordered hemispherical silver nanocap arrays templated by porous anodic alumina (PAA) membranes as robust and cost-efficient surface-enhanced Raman scattering (SERS) substrates. This geometry produces a high Raman signal due to its periodic hexagonal arrangements and control of the gap between the nanostructures in the sub-10-nm regime. The surface structure can be tuned further to optimize the enhancement factor according to optional PAA fabrication and silver deposition parameters. Finite-difference time-domain calculations indicate that the structure may possess excellent SERS characteristics due to the high density and abundance of hot spots.