Within-winter flexibility in muscle and heart lipid transport and catabolism in passerine birds.

Small birds in cold climates may show within-winter metabolic flexibility to match metabolic capacities to prevailing weather conditions. This flexibility may occur over periods of days to weeks, but the underlying mechanisms for such flexibility are not well understood. Because lipids are the primary fuel for sustained thermogenesis, we examined whether lipid transport and catabolism can mediate within-winter metabolic flexibility in two small temperate-zone wintering passerine birds, dark-eyed juncos (Junco hyemalis) and house sparrows (Passer domesticus). We used simple and multiple regression analyses to test for correlations of several lipid transporters in pectoralis muscle (plasma membrane-bound and cytosolic fatty acid-binding proteins, FABP; fatty acyl translocase, FAT/CD36) and regulatory enzymes (carnitine acyl transferase, CPT; ß-hydroxyacyl CoA dehydrogenase, HOAD) in pectoralis and heart with short-term (ST, 0-7 days), medium-term (MT, 14-30 days) and long-term (LT, 30-year mean daily and extreme minimum temperatures, day of winter season) temperature variables. We hypothesized negative correlations between these regulators and temperature variables. Juncos showed negative correlations for FABPs with ST or MT temperature variables, but other lipid transporters and regulatory enzymes showed positive correlations with ST or MT temperatures for juncos, suggesting no consistent pathway-wide response to within-winter temperatures. LT temperature variables showed several significant associations with lipid transporters and enzymes for juncos, but also not in consistent directions. House sparrows showed the expected negative correlations with LT temperatures for FABPpm, but positive correlations with temperature variables for FABPc, CPT and HOAD. Different species-specific patterns of variation and the absence of consistent pathway-wide responses to temperature suggest that the lipid transport and catabolism pathway is not a uniform mediator of within-winter metabolic flexibility among small birds.

Within-Winter Flexibility in Muscle Masses, Myostatin, and Cellular Aerobic Metabolic Intensity in Passerine Birds.

Metabolic rates of passerine birds are flexible traits that vary both seasonally and among and within winters. Seasonal variation in summit metabolic rates (Msum = maximum thermoregulatory metabolism) in birds is consistently correlated with changes in pectoralis muscle and heart masses and sometimes with variation in cellular aerobic metabolic intensity, so these traits might also be associated with shorter-term, within-winter variation in metabolic rates. To determine whether these mechanisms are associated with within-winter variation in Msum, we examined the effects of short-term (ST; 0-7 d), medium-term (MT; 14-30 d), and long-term (LT; 30-yr means) temperature variables on pectoralis muscle and heart masses, pectoralis expression of the muscle-growth inhibitor myostatin and its metalloproteinase activators TLL-1 and TLL-2, and pectoralis and heart citrate synthase (CS; an indicator of cellular aerobic metabolic intensity) activities for two temperate-zone resident passerines, house sparrows (Passer domesticus) and dark-eyed juncos (Junco hyemalis). For both species, pectoralis mass residuals were positively correlated with ST temperature variables, suggesting that cold temperatures resulted in increased turnover of pectoralis muscle, but heart mass showed little within-winter variation for either species. Pectoralis mRNA and protein expression of myostatin and the TLLs were only weakly correlated with ST and MT temperature variables, which is largely consistent with trends in muscle masses for both species. Pectoralis and heart CS activities showed weak and variable trends with ST temperature variables in both species, suggesting only minor effects of temperature variation on cellular aerobic metabolic intensity. Thus, neither muscle or heart masses, regulation by the myostatin system, nor cellular aerobic metabolic intensity varied consistently with winter temperature, suggesting that other factors regulate within-winter metabolic variation in these birds.