ABSTRACT
BACKGROUND: Cysteinyl-leukotrienes are mediators of inflammatory responses in bronchial asthma. We studied the genes encoding the enzymes involved in their synthesis to identify risk factors for asthma. The promoter polymorphisms LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 have been reported to be associated with bronchial asthma. METHODS: We analyzed the effects of LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 on asthma susceptibility by means of a case-control study with 193 ethnically matched, unrelated individuals. Participants were classified as severe asthmatics, nonsevere asthmatics, and nonasthmatics, using a combination of 2 techniques: polymerase chain reaction-restricted fragment length polymorphism and multiplex capillary electrophoresis. RESULTS: No association was found between these polymorphisms and asthma, neither individually nor in combination. CONCLUSION: Although the studied polymorphisms have been previously reported to constitute risk factors for the disease, we found no association between LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 polymorphisms and bronchial asthma.
Subject(s)
Arachidonate 5-Lipoxygenase/genetics , Asthma/genetics , Asthma/physiopathology , Carrier Proteins/genetics , Glutathione Transferase/genetics , Membrane Proteins/genetics , 5-Lipoxygenase-Activating Proteins , Adolescent , Adult , Aged , Arachidonate 5-Lipoxygenase/immunology , Arachidonate 5-Lipoxygenase/metabolism , Asthma/diagnosis , Asthma/enzymology , Asthma/immunology , Carrier Proteins/immunology , Carrier Proteins/metabolism , Case-Control Studies , Disease Progression , Female , Gene Frequency , Genetic Association Studies , Genetic Predisposition to Disease , Genotype , Glutathione Transferase/immunology , Glutathione Transferase/metabolism , Humans , Inflammation Mediators/immunology , Male , Membrane Proteins/immunology , Membrane Proteins/metabolism , Middle Aged , Polymorphism, Genetic , SpainSubject(s)
Drug Hypersensitivity/etiology , Proton Pump Inhibitors/adverse effects , Drug Hypersensitivity/diagnosis , Erythema/etiology , Female , Gastroenteritis/etiology , Humans , Middle Aged , Molecular Mimicry , Nephrectomy , Proton Pump Inhibitors/administration & dosage , Proton Pump Inhibitors/chemistry , Pruritus/etiology , Skin Tests , Vomiting/etiologyABSTRACT
We have identified the integrin alpha(v)beta3 as a ligand for mouse gp49B1, thus identifying a new class of ligand for a member of the family of inhibitory immunoreceptors that bear C2-type immunoglobulin-like domains. The specific interaction was shown by both cell-protein and cell-cell binding assays. In addition, we found that the interaction of alpha(v)beta3 with gp49B1 on bone marrow-derived mouse mast cells inhibited antigen-induced immunoglobulin E-mediated cell activation. Because neither gp49B1 nor alpha(v)beta3 exhibit substantive allelic variation, their newly appreciated interaction may reflect an innate pathway for down-regulating the activity of mast cells.
Subject(s)
Antigens, Surface/metabolism , Integrins/metabolism , Mast Cells/immunology , Membrane Glycoproteins/metabolism , Receptors, Immunologic/metabolism , Receptors, Vitronectin/metabolism , Animals , Antigens, Surface/genetics , Binding, Competitive , Bone Marrow Cells/immunology , Immunoglobulin E/immunology , Integrins/genetics , Ligands , Membrane Glycoproteins/genetics , Mice , Protein Binding , Receptors, Immunologic/genetics , Recombinant Proteins/metabolism , Vitronectin/metabolismABSTRACT
BACKGROUND: The calcitonin is an hormone produced by the thyroid gland C cells. The salmon calcitonin is used in some osteomuscular diseases. There are few references of allergic reaction to this hormone. We introduce a case of a sixty years old woman with several previous episodes of rhinitis, conjunctivitis and perspiration immediately after the administration of salmon calcitonin with nasal spray or intramuscular administration (Calsynar). There were some temporal periods of good tolerance between these episodes. METHODS: Skin prick test (SPT), nasal and intramuscular challenge test with commercial salmon calcitonin (Miacalcic) were performed. Leukocyte histamine release test with salmon calcitonin and serum tryptase levels at baseline and after intramuscular challenge test were performed. RESULTS: The patient skin prick test with commercial calcitonin (Miacalcic. 50 UI/ml) was positive and negative in controls. The nasal challenge test with a calcitonin nasal spray, up to 150 UI, was negative. The intramuscular challenge test with 25 UI of Miacalcic was positive with an immediate anaphylactic reaction. Whole blood histamine release studies were negative. Serum tryptase levels after intramuscular challenge did not increase significantly with regard to the basal levels. CONCLUSION: We have introduced a case of anaphylaxis by calcitonin that suggest an IgE mediated hypersensitivity reaction.
