ABSTRACT
Y-1 cells were passaged weekly for 6 wk in MACT IV medium without a period of adaptation to this basal medium that requires 1% dialyzed fetal bovine serum as a supplement. The steroid pathway and chromosome number, reported earlier for this cell line under culture conditions that used much higher concentrations of nondialyzed sera, remained stable despite dialysis and reduction of the fetal bovine serum to 1%.
Subject(s)
Adrenal Cortex Hormones/metabolism , Adrenal Cortex Neoplasms/metabolism , Chromosomes/ultrastructure , Culture Media/pharmacology , Adrenal Cortex Neoplasms/ultrastructure , Animals , Cell Line , Karyotyping , MiceABSTRACT
An improved basal medium is presented that required only minimal supplementation with dialyzed fetal bovine serum or bovine serum albumin and fetuin to be comparable to Ham's F-10, which requires 15% horse serum (HS) and 2.5% fetal bovine serum (FBS) for the growth and function of Y-1, mouse adrenal cortex tumor, cells. Cell monolayers maintained for up to 2 weeks without any protein supplementation have retained their steroid response to ACTH. The medium differs from Ham's F-10 in its buffer composition and higher calcium-ion concentration. This medium should be a useful adjunct to studies pertaining to steroid and lipid intermediary metabolism, the retention of a specialized physiological function in a chemically defined medium, and the mechanism of hormonal response.
Subject(s)
Adrenal Gland Neoplasms , Calcium/pharmacology , Cell Line , Culture Media , Adrenocorticotropic Hormone/pharmacology , Animals , Cell Division/drug effects , Mice , Mice, Inbred Strains , Neoplasm Proteins/biosynthesis , Steroids/biosynthesisABSTRACT
The surface topologies of mouse adrenal cortex tumor cells of primary or clonal origin grown as monolayer cell cultures were observed by scanning electron microscopy following their exposure to substances that effect steroid release and/or cell rounding. ACTH induced cell rounding with a concomitant profuse development of fine microvilli in a non-synchronously dividing cell population. This was less pronounced with other steroidogenic substances and absent in EGTA or trypsin-treated cells. Morphological alterations occurred most rapidly with cAMP and least rapidly with dbcAMP. The rapid development of fine microvilli with ACTH is proposed to be a specific hormone mediated response.
