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1.
Infect Genet Evol ; 41: 84-92, 2016 07.
Article in English | MEDLINE | ID: mdl-27057620

ABSTRACT

Most indigenous ethnias from Northern Argentina live in rural areas of "the Gran Chaco" region, where Trypanosoma cruzi is endemic. Serological and parasitological features have been poorly characterized in Aboriginal populations and scarce information exist regarding relevant T. cruzi discrete typing units (DTU) and parasitic loads. This study was focused to characterize T. cruzi infection in Qom, Mocoit, Pit'laxá and Wichi ethnias (N=604) and Creole communities (N=257) inhabiting rural villages from two highly endemic provinces of the Argentinean Gran Chaco. DNA extracted using Hexadecyltrimethyl Ammonium Bromide reagent from peripheral blood samples was used for conventional PCR targeted to parasite kinetoplastid DNA (kDNA) and identification of DTUs using nuclear genomic markers. In kDNA-PCR positive samples from three rural Aboriginal communities of "Monte Impenetrable Chaqueño", minicircle signatures were characterized by Low stringency single primer-PCR and parasitic loads calculated using Real-Time PCR. Seroprevalence was higher in Aboriginal (47.98%) than in Creole (27.23%) rural communities (Chi square, p=4.e(-8)). A low seroprevalence (4.3%) was detected in a Qom settlement at the suburbs of Resistencia city (Fisher Exact test, p=2.e(-21)).The kDNA-PCR positivity was 42.15% in Aboriginal communities and 65.71% in Creole populations (Chi square, p=5.e(-4)). Among Aboriginal communities kDNA-PCR positivity was heterogeneous (Chi square, p=1.e(-4)). Highest kDNA-PCR positivity (79%) was detected in the Qom community of Colonia Aborigen and the lowest PCR positivity in two different surveys at the Wichi community of Misión Nueva Pompeya (33.3% in 2010 and 20.8% in 2014). TcV (or TcII/V/VI) was predominant in both Aboriginal and Creole communities, in agreement with DTU distribution reported for the region. Besides, two subjects were infected with TcVI, one with TcI and four presented mixed infections of TcV plus TcII/VI. Most minicircle signatures clustered according to their original localities, but in a few cases, signatures from one locality clustered with signatures from other village, suggesting circulation of the same strains in the area. Parasitic loads ranged from undetectable to around 50 parasite equivalents/mL, showing higher values than those generally observed in chronic Chagas disease patients living in urban centers of Argentina. Our findings reveal the persistence of high levels of infection in these neglected populations.


Subject(s)
Chagas Disease/epidemiology , DNA, Kinetoplast/genetics , Endemic Diseases , Phylogeny , Trypanosoma cruzi/genetics , Adolescent , Adult , Aged , Argentina/epidemiology , Chagas Disease/ethnology , Chagas Disease/parasitology , Child , Child, Preschool , Humans , Indians, South American , Infant , Middle Aged , Parasite Load , Rural Population , Seroepidemiologic Studies , Trypanosoma cruzi/classification , Trypanosoma cruzi/growth & development , Trypanosoma cruzi/pathogenicity , Vulnerable Populations
2.
Am J Transplant ; 13(12): 3253-61, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24266974

ABSTRACT

Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36-98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.


Subject(s)
Chagas Disease/diagnosis , Organ Transplantation/adverse effects , Adolescent , Adult , Aged , Algorithms , DNA, Kinetoplast/genetics , Female , Genotype , Humans , Male , Middle Aged , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Time Factors , Tissue Donors , Trypanosoma cruzi/genetics , Young Adult
3.
Acta Trop ; 124(1): 79-86, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22771688

ABSTRACT

Little is known about the sylvatic transmission cycle of Trypanosoma cruzi in the Gran Chaco ecoregion. We conducted surveys to identify the main sylvatic hosts of T. cruzi, parasite discrete typing units and vector species involved in Pampa del Indio, a rural area in the humid Argentinean Chaco. A total of 44 mammals from 14 species were captured and examined for infection by xenodiagnosis and polymerase chain reaction amplification of the hyper-variable region of kinetoplast DNA minicircles of T. cruzi (kDNA-PCR). Ten (22.7%) mammals were positive by xenodiagnosis or kDNA-PCR. Four of 11 (36%) Didelphis albiventris (white-eared opossums) and six of nine (67%) Dasypus novemcinctus (nine-banded armadillos) were positive by xenodiagnosis and or kDNA-PCR. Rodents, other armadillo species, felids, crab-eating raccoons, hares and rabbits were not infected. Positive animals were highly infectious to the bugs that fed upon them as determined by xenodiagnosis. All positive opossums were infected with T. cruzi I and all positive nine-banded armadillos with T. cruzi III. Extensive searches in sylvatic habitats using 718 Noireau trap-nights only yielded Triatoma sordida whereas no bug was collected in 26 light-trap nights. Four armadillos or opossums fitted with a spool-and-line device were successfully tracked to their refuges; only one Panstrongylus geniculatus was found in an armadillo burrow. No sylvatic triatomine was infected with T. cruzi by microscopical examination or kDNA-PCR. Our results indicate that two independent sylvatic transmission cycles of T. cruzi occur in the humid Chaco. The putative vectors of both cycles need to be identified conclusively.


Subject(s)
Chagas Disease/parasitology , Chagas Disease/veterinary , Disease Reservoirs , Disease Vectors , Trypanosoma cruzi/isolation & purification , Trypanosoma cruzi/pathogenicity , Animals , Animals, Wild , Argentina , Chagas Disease/transmission , DNA, Kinetoplast/chemistry , DNA, Kinetoplast/genetics , DNA, Protozoan/chemistry , DNA, Protozoan/genetics , Humans , Humidity , Life Cycle Stages , Molecular Sequence Data , Polymerase Chain Reaction , Rural Population , Sequence Analysis, DNA
4.
Parasitology ; 139(4): 516-21, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22309735

ABSTRACT

Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population.


Subject(s)
Chagas Disease/epidemiology , Chagas Disease/parasitology , Endemic Diseases , Trypanosoma cruzi/classification , Trypanosoma cruzi/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Argentina/epidemiology , Chagas Cardiomyopathy/epidemiology , Chagas Cardiomyopathy/parasitology , Chagas Cardiomyopathy/physiopathology , Chagas Disease/physiopathology , Child , Child, Preschool , DNA, Protozoan/analysis , DNA, Protozoan/genetics , Female , Genetic Variation , Genotype , Heart/parasitology , Humans , Infant , Male , Middle Aged , Phylogeny , Polymerase Chain Reaction , Trypanosoma cruzi/isolation & purification , Young Adult
6.
J Oral Rehabil ; 31(8): 790-7, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15265216

ABSTRACT

This in vitro study was conducted to evaluate the interaction between the shear bond strength and the surface treatment method for a commercial dental ceramic. Ninety bonded ceramic units were manufactured for this study. Each unit was made by luting two cylinder-shaped ceramic samples to each other with a resin-composite luting agent. The units were then divided into nine groups, containing 10 units in each group. Samples from each group were treated with one of the following: etching with 4.9% hydrofluoric acid for 10, 20 and 40 s, 9.5% hydrofluoric acid for 10, 20 and 40 s, 40% orthophosphoric acid for 40 s, air abrasion with alumina in 50-microm particles, and grinding with a high-speed diamond bur. The treated samples were then silanated and luted with a resin-composite luting agent. The luted units were then loaded to failure. Two samples from each group were neither silanated nor luted after the surface treatment procedure, and morphological changes obtained by various surface treatment regimens were investigated by scanning electron microscopy. A statistically significant difference was observed among the mean shear bond strengths of the groups prepared with different surface treatment techniques (P = 0.00). Hydrofluoric acid appeared to be the most suitable chemical medium to produce a reliable ceramic bond. Etching time and concentration of the acidic medium were also observed as important prognostic variates. Orthophosphoric acid treatment was observed to be the least effective surface treatment method on the heat-pressed ceramic samples. Physical applications such as bur grinding and air blasting maintained stronger bonds than the orthophosphoric acid, while producing weaker bonds than surfaces treated with hydrofluoric acid in all concentrations and etching periods. The effect of the silane priming agent was not considered in this study.


Subject(s)
Dental Bonding , Dental Porcelain , Dental Veneers , Hot Temperature , Stress, Mechanical
7.
Encephale ; 29(6): 527-34, 2003.
Article in French | MEDLINE | ID: mdl-15029087

ABSTRACT

Several studies have found a significant increase in the prevalence of some personality disorders in the first-degree relatives of patients with schizophrenia; other studies have found subtle neuropsychological deficits in these relatives. However, little is known about the specificity of the personality traits reported or about the relationship between these traits and the neuropsychological deficits. One hundred first-degree relatives of patients with schizophrenia (AS) and 88 first-degree relatives of affective psychotic (APA) patients completed the Eysenck Personality Questionnaire which measures extraversion, neuroticism, psychoticism. They were also administered the National Adult Reading Test (NART), the Trail Making Test (TMT) and a Verbal Fluency Test (VFT). In the AS group, the male relatives scored significantly higher on the psychoticism scale than the male relatives in the APA group. There were no significant differences in personality between female relatives of the 2 patients groups. In the AS group, the NART scores were superior when the psychoticism scores were lower and the TMT performance was better when the extraversion scores were higher. These results seem to indicate some specificity as well as sex differences of the psychoticism dimension. Moreover, the relationship between the personality dimensions and the neuropsychological performance could indicate that psychoticism increases vulnerability to schizophrenia whereas extraversion decreases it.


Subject(s)
Cognition Disorders/diagnosis , Personality Disorders/diagnosis , Personality Disorders/epidemiology , Personality Inventory , Psychotic Disorders/genetics , Schizophrenia/genetics , Adult , Cognition Disorders/epidemiology , Female , Humans , Male , Neuropsychological Tests , Personality Disorders/psychology , Severity of Illness Index
8.
J Prosthet Dent ; 86(4): 438-40, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11677541

ABSTRACT

It is not possible for the wax pattern of most large-volume maxillary resection obturators to be placed into a flask for conventional compression molding processing and positioning of the wax pattern. However, these wax patterns and their channel access design often can be modified and aligned in position for processing with the continuous pressure injection technique. This article describes the use of the continuous pressure injection technique to process the perpendicularly aligned wax pattern of a maxillary resection obturator. It also describes a modified channel design and a simple method to pull back the injection funnel to increase the volume of the flask.


Subject(s)
Maxilla/surgery , Palatal Obturators , Prosthesis Design/methods , Calcium Sulfate , Dental Materials , Humans , Injections/instrumentation , Polymethyl Methacrylate , Pressure , Prosthesis Design/instrumentation , Surface Properties , Waxes
11.
Eur J Nucl Med ; 26(1): 8-11, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9933655

ABSTRACT

Considering the current need to improve cost-effectiveness in cancer patient management, a prospective study was undertaken in order to define the optimal combination of bone scan and tumour marker assays in breast and lung cancer strategies, as has been done in the case of prostate cancer. All patients with breast or lung cancer referred to the Nuclear Medicine Department of the Grenoble Teaching Hospital between December 1995 and April 1997 were included. A blood sample was drawn in each case for marker assay (CA15-3 or CEA and CYFRA 21-1) on the same day as the bone scan. Two hundred and seventy-five patients were included: 118 with lung cancer and 157 with breast cancer. With regard to lung cancer, no information useful for guiding bone scan prescription was obtained through CEA and CYFRA 21-1 assays. For breast cancer, the results suggest that in asymptomatic patients, a CA15-3 level of less than 25 U/ml (upper normal value chosen as the threshold) is strongly predictive of a negative bone scan; by contrast, high tumour marker levels are predictive of neoplastic bone involvement. When a doubtful bone scan is obtained in a patient with breast cancer, a normal marker level makes it highly probable that bone scan abnormalities are not related to malignancy.


Subject(s)
Biomarkers, Tumor/blood , Bone Neoplasms/diagnostic imaging , Bone Neoplasms/secondary , Bone and Bones/diagnostic imaging , Breast Neoplasms, Male/pathology , Breast Neoplasms/pathology , Lung Neoplasms/pathology , Aged , Aged, 80 and over , Antigens, Neoplasm/blood , Bone Neoplasms/diagnosis , Carcinoembryonic Antigen/blood , Female , Humans , Keratin-19 , Keratins , Male , Middle Aged , Mucin-1/blood , Predictive Value of Tests , Prospective Studies , Radionuclide Imaging , Radiopharmaceuticals , Technetium Tc 99m Medronate
12.
Quintessence Int ; 24(3): 177-9, 1993 Mar.
Article in English | MEDLINE | ID: mdl-8511277

ABSTRACT

A cantilevered adhesive prosthesis was designed for a patient whose maxillary right central incisor was extracted. The width of the edentulous space was much greater than the mesiodistal width of the lost tooth, impeding the construction of a conventional fixed partial denture. A much more conservative and esthetic result was achieved by the construction of a cantilevered adhesive prosthesis in which the extracted tooth was used as a pontic.


Subject(s)
Denture Design , Denture, Partial, Fixed, Resin-Bonded , Humans , Incisor , Male , Maxilla , Middle Aged , Tooth Extraction
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