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1.
Ann N Y Acad Sci ; 939: 297-308, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11462783

ABSTRACT

In assessing plasma concentrations of drugs in relation to neuroprotective effect, emphasis should be placed on measured or calculated concentrations during the window of opportunity for effect, rather than at the end of the experiment. Unbound (plasma free) concentrations should be especially considered as should brain penetration to the stroked area. Problem-solving exercises should include post hoc assessment of dosing residues and proof of exposure. The shape of the graph of response versus concentration in plasma is very steep, giving the impression of an all-or-none effect. Although higher doses lead to greater effects, attempts to statistically correlate plasma level and infarct size are likely to be unsuccessful. There is strong evidence that the pharmacokinetic properties of drugs are affected by the physiological consequences of ischemia.


Subject(s)
Excitatory Amino Acid Antagonists/blood , Infarction, Middle Cerebral Artery/blood , Models, Animal , Neuroprotective Agents/blood , Pyridines/blood , Animals , Area Under Curve , Brain/metabolism , Callithrix , Cats , Excitatory Amino Acid Antagonists/pharmacokinetics , Gerbillinae , Humans , Neuroprotective Agents/pharmacokinetics , Pyridines/pharmacokinetics , Rats , Species Specificity
2.
Electroencephalogr Clin Neurophysiol ; 106(3): 244-50, 1998 Mar.
Article in English | MEDLINE | ID: mdl-9743283

ABSTRACT

Diffuse axonal injury (DAI) is an important cause of morbidity and mortality after traumatic brain injury (TBI), and its severity is therefore a major determinant of outcome. There have been suggestions that the extent of DAI may be reflected in quantitative measures of cerebral function, including the electroencephalogram (EEG) and brain-stem auditory evoked potentials (BAEPs). It has therefore been proposed that these quantitative methods of analysis may provide objective predictors of outcome following TBI. We prospectively investigated the relationship between quantitative EEG and BAEP measures and outcome, in 60 comatose patients (47 male and 13 female; age range 1-80 years, mean 36.4) after severe, closed head injury (post-resuscitation Glasgow Coma Scale (GCS) of 8). The Spearman correlation coefficients (rs) have been calculated for quantitative EEG measures (mean regional power and interhemispheric coherence) and BAEPs with patient outcome on the Glasgow Outcome and Disability Rating Scales at 6 months and 1 year. The measures most significantly correlated with outcome (P < 0.001) are over the left hemisphere, beta activity power (amplitude squared) in the fronto-central and centro-temporal regions, and alpha activity power in the centro-temporal region. We found no correlation between interhemispheric coherence (a statistical measure of cross-correlation in the frequency domain) and outcome at either 6 months or 1 year post-injury. In 10 fatalities, we examined the relationship between interhemispheric EEG coherence prior to deaths and the histopathological severity of DAI, in concordant regions. The only significant correlation between DAI and interhemispheric coherence is seen in the alpha band at the temporo-occipital site (rs = -0.79, P = 0.007). Our data indicate that there is regional information in EEG power spectra over the left hemisphere, which could be used in prognostic predictions for patients in coma after severe TBI. We were unable to demonstrate a correlation between interhemispheric coherence and outcome, or any clear and consistent evidence of a relationship between interhemispheric coherence and the severity of DAI.


Subject(s)
Coma/physiopathology , Craniocerebral Trauma/physiopathology , Electroencephalography , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Coma/etiology , Coma/mortality , Coma/pathology , Craniocerebral Trauma/complications , Craniocerebral Trauma/mortality , Craniocerebral Trauma/pathology , Evoked Potentials, Auditory, Brain Stem , Female , Glasgow Coma Scale , Humans , Infant , Male , Middle Aged , Prognosis , Prospective Studies
3.
J Clin Pharmacol ; 37(5): 416-25, 1997 May.
Article in English | MEDLINE | ID: mdl-9156374

ABSTRACT

The pharmacokinetic properties of the lactate-lowering drug dichloroacetate were investigated in 111 adult patients with lactic acidosis who were randomized to receive dichloroacetate as part of a placebo-controlled clinical trial. The clinical symptoms and etiology of lactic acidosis varied markedly among patients. Dichloroacetate, at a dose of 50 mg per kilogram of body weight, was administered in a 30-minute intravenous infusion into a peripheral vein. A second dose, identical to the first, was administered 2 hours after beginning the first infusion. Plasma levels of dichloroacetate were determined from blood samples collected periodically up to 288 hours after administration and the data were subjected to pharmacokinetic modeling. The pharmacokinetic properties of dichloroacetate in these acutely ill patients were complex and differed markedly from those in healthy volunteers, whose data fitted a one-compartment pharmacokinetic model. In contrast, the data from patients fitted one-, two-, or three-compartment pharmacokinetic models or even none of these, depending on the individual. Drug clearance in plasma tended to decrease as the number of compartments required to fit the data increased or as the number of drug treatments increased.


Subject(s)
Acidosis, Lactic/metabolism , Dichloroacetic Acid/pharmacokinetics , Acidosis, Lactic/drug therapy , Adolescent , Adult , Aged , Dichloroacetic Acid/therapeutic use , Female , Humans , Male , Middle Aged
5.
Intensive Care Med ; 22(1): 39-46, 1996 Jan.
Article in English | MEDLINE | ID: mdl-8857436

ABSTRACT

OBJECTIVE: To determine the prognostic value of multimodal evoked potentials (EPs) and event-related (ERPs) potentials in coma (Glasgow Coma Score <8), after severe traumatic brain injury (TBI). DESIGN: Prospective, longitudinal study of neurophysiological responses recorded during traumatic coma. SETTING: Intensive Care Unit, Frenchay Hospital, Bristol, UK. PARTICIPANTS: Fifty-four comatose TBI patients (age range 1-80 years, mean 36.4). METHODS: Neurophysiological responses were recorded from 11 scalp electrodes with earlobe reference. Conduction times were measured for brainstem auditory, flash visual and somatosensory, short-latency EPs. Peak latencies and amplitudes were determined for long-latency components of visual and auditory ERPs, generated by passive "oddball" paradigms. These neurophysiological and various clinical parameters were correlated with patient outcome using Pearson's coefficient. MAIN OUTCOME MEASURE: Three month Glasgow Outcome Scale (GOS). RESULTS AND CONCLUSION: Highly significant (P <0.001) correlations exist between long-latency ERP components and 3-month outcome. Short-latency EPs, brainstem (wave I-V) and somatosensory conduction times also correlate significantly with the GOS (P <0.01). Of the clinical measurements, pupillary response patterns, APACHE II and Glasgow Coma Scores (GCS) correlate significantly with outcome, as do the retrospective measures of duration of coma and post-traumatic amnesia (PTA) in survivors. Unfortunately, due to variance of long-latency responses, even in controls, absolute values cannot be relied upon as prognosticators. The presence of "mismatch negativity" predicted the return of consciousness (89.7% sensitivity and 100% specificity) and preceded changes in GCS. Its latency was the single best indicator of 90-day outcome from coma (r = -0.641).


Subject(s)
Brain Injuries/complications , Coma/diagnosis , Evoked Potentials/physiology , Acoustic Stimulation , Adolescent , Adult , Aged , Brain Injuries/diagnostic imaging , Case-Control Studies , Child , Child, Preschool , Coma/etiology , Electroencephalography , Female , Glasgow Coma Scale , Humans , Infant , Longitudinal Studies , Male , Middle Aged , Photic Stimulation , Predictive Value of Tests , Prognosis , Reaction Time , Regression Analysis , Severity of Illness Index , Tomography, X-Ray Computed
7.
J Med Eng Technol ; 19(2-3): 42-51, 1995.
Article in English | MEDLINE | ID: mdl-7494208

ABSTRACT

This paper describes a rationale and methodology for the use of DC recording techniques to reveal patterns of cerebral functional specialization. Results from a number of recent experiments are described. In each of these studies 15 channels of DC potential were recorded from a widely spaced array of scalp-mounted Ag/AgCl electrodes. The duration of single trial recording was always 40 s although the paradigms involved continuous performance of a complex cognitive task. In this series of experiments we used the following tasks: both visually and aurally presented word target detection with classification on a semantic basis, recognition of 'famous' faces and discrimination of animal versus non-animal sounds. In each of these tasks the rate of presentation has been systematically varied to evaluate quantitative aspects of task demand. In general, the results from these studies demonstrate that DC recording does provide a method for assessing the degree of activation of different cortical regions at the same time. However, it appears that additional transformation of the data may be required to separate out a number of overlapping slow potentials to increase the sensitivity to more subtle aspects of the cognitive demand. Two different methods to perform the separation are described and applied.


Subject(s)
Cerebral Cortex/physiology , Cognition/physiology , Electroencephalography/methods , Adult , Auditory Perception/physiology , Brain Mapping , Data Interpretation, Statistical , Electricity , Electrodes , Electromagnetic Fields , Female , Humans , Male , Membrane Potentials/physiology , Middle Aged , Reference Values , Visual Perception/physiology
8.
J Pharm Sci ; 84(2): 174-8, 1995 Feb.
Article in English | MEDLINE | ID: mdl-7738796

ABSTRACT

The O-saccharinylmethyl prodrug of 17 beta-estradiol was about nine times as potent, based on 50% effective dose (ED50) values, as 17 beta-estradiol when each was given as an oral dose to ovariectomized rats. Similarly, a significant lowering of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels at 24 h was observed when an ED50 dose of the prodrug was given but not when an equimolar dose of 17 beta-estradiol was given orally. However, when given intravenously, there was no difference in potency between the two drugs. In the bioavailability studies, a significantly longer half-life (approximately 5-7 times) for 17B-estradiol was observed when the prodrug was given orally than when 17 beta-estradiol was given orally or when the prodrug or 17 beta-estradiol were given intravenously. This result was consistent with an observed five-fold enhancement in the oral bioavailability of 17 beta-estradiol when the prodrug was given.


Subject(s)
Estradiol/analogs & derivatives , Estradiol/pharmacokinetics , Prodrugs/pharmacokinetics , Saccharin/analogs & derivatives , Administration, Oral , Animals , Biological Availability , Estradiol/administration & dosage , Estradiol/pharmacology , Female , Follicle Stimulating Hormone/blood , Half-Life , Injections, Intravenous , Luteinizing Hormone/blood , Organ Size/drug effects , Ovariectomy , Prodrugs/administration & dosage , Rats , Rats, Sprague-Dawley , Saccharin/administration & dosage , Saccharin/pharmacokinetics , Saccharin/pharmacology , Uterus/drug effects
9.
J Pharm Sci ; 84(1): 111-4, 1995 Jan.
Article in English | MEDLINE | ID: mdl-7714731

ABSTRACT

We examined promazine pharmacokinetics in nine patients with hepatic cirrhosis and in six healthy subjects. A specific and sensitive HPLC method was used to measure promazine concentrations in plasma, plasma water (free drug), red blood cells, and urine after oral administration of promazine (2 x 50 mg tablet). There were highly significant reductions in total plasma clearance (p < 0.01), free drug total plasma clearance (p < 0.01), metabolic clearance (p < 0.01), metabolic clearance of free drug (p < 0.01), and fraction bound (p < 0.01) in the cirrhotic patients. The elimination half-life and the area under the plasma concentration-time curve were significantly increased (p < 0.001 and p < 0.05, respectively) in the cirrhotic patients. However, the overall excreted promazine in urine, time to the promazine peak concentration, distribution half-life, renal clearance, apparent volume of distribution, and the promazine concentration ratio between plasma and red blood cells were not different. Thus caution is needed in using promazine for patients with hepatic cirrhosis. A newly developed galactose single point (GSP) method was applied to quantitatively measure the residual liver function in cirrhosis patients and successfully correlated it with promazine elimination half-life (r = 0.770, p < 0.01), total plasma clearance of free drug (r = 0.899, p < 0.005), metabolic clearance of free drug (r = 0.902, p < 0.005), and plasma protein binding (r = 0.822, p < 0.005). GSP may be a convenient index for promazine routine dosage adjustment in patients with liver cirrhosis.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Galactose/pharmacokinetics , Liver Cirrhosis/metabolism , Promazine/pharmacokinetics , Adult , Blood Proteins/metabolism , Half-Life , Humans , Liver Function Tests , Male , Protein Binding
10.
Regul Pept ; 55(2): 179-88, 1995 Jan 26.
Article in English | MEDLINE | ID: mdl-7754104

ABSTRACT

Rats were given i.v., intranasal or intraperitoneal doses of CCK-8 (sulfated) labelled with 125I-labeled Bolton and Hunter reagent. Radioactivity was found mainly in the liver, kidney, and the intestinal contents. No radioactivity was detected in the brain. In animals dosed i.v., specific localization occurred in the tissue of the pyloric region of the stomach, and in the pancreas. Label persisted within the pyloric region of the stomach for longer than 30 min, in spite of the reported half-life of CCK-8 in plasma of approximately 1 min. Intranasal and intraperitoneal doses had limited bioavailability. The binding to the sites in the pyloric region of the stomach, which required systemic delivery, may have identified receptors associated with appetite control.


Subject(s)
Sincalide/analysis , Administration, Intranasal , Amino Acid Sequence , Animals , Autoradiography , Biological Availability , Brain/metabolism , Female , Frozen Sections , Injections, Intraperitoneal , Injections, Intravenous , Intestinal Mucosa/metabolism , Intestines/anatomy & histology , Iodine Isotopes , Isotope Labeling , Kidney/metabolism , Liver/metabolism , Molecular Sequence Data , Pancreas/anatomy & histology , Pancreas/metabolism , Pylorus/anatomy & histology , Pylorus/metabolism , Rats , Rats, Inbred F344 , Sincalide/administration & dosage , Sincalide/chemistry , Sincalide/pharmacokinetics , Tissue Distribution
12.
Am J Vet Res ; 55(10): 1428-33, 1994 Oct.
Article in English | MEDLINE | ID: mdl-7998701

ABSTRACT

A specific, sensitive, reverse-phase high-performance liquid chromatographic assay for acepromazine, with analytic sensitivity as low as 5 ng/ml of plasma, and electrochemical detection with an oxidation potential of 0.7 V, was used to study the pharmacokinetics of acepromazine given at a dosage of 0.15 mg/kg of body weight in horses. The relation between effect and pharmacokinetics of the drug was examined. The effects studied included those on blood pressure, pulse, PCV, measures of respiration function, and sedation. Intravenously administered doses led to a biphasic concentration decay pattern with an alpha-phase distribution half-life of < 3 minutes. The beta-phase half-life was in the range of 50 to 150 minutes. The CNS effects peaked at 20 minutes after administration, and the hemodynamic effects peaked at 100 minutes. In all horses, the most sensitive variable was the PCV, which decreased by up to 20% (P < 0.0001). Systolic, diastolic, and mean blood pressures decreased (P < 0.0001); heart rate was unchanged (P > 0.05). Neither blood gas tensions nor blood pH changed noticeably (P > 0.05). In all horses studied, acepromazine had a significant (P < 0.0001) sedative effect, as observed by posture and alertness. None of the observed pharmacodynamic effects correlated well with plasma acepromazine concentration. These effects persisted beyond the time of detectable acepromazine concentration, indicating that they might be caused by active metabolites, or that their timing could result from complex pharmacokinetic compartment influences.


Subject(s)
Acepromazine/pharmacology , Acepromazine/pharmacokinetics , Horses/physiology , Animals , Arousal/drug effects , Blood Gas Analysis/veterinary , Blood Pressure/drug effects , Chromatography, High Pressure Liquid/veterinary , Female , Heart Rate/drug effects , Hematocrit/veterinary , Horses/blood , Hydrogen-Ion Concentration , Male
13.
Am J Med ; 97(1): 47-54, 1994 Jul.
Article in English | MEDLINE | ID: mdl-8030656

ABSTRACT

STUDY OBJECTIVE: To determine the pathogenesis and clinical course of lactic acidosis in adults receiving standard medical care. DESIGN: Placebo arm of a 5-year prospective, randomized, blinded study comparing placebo and dichloroacetate as specific lactate-lowering therapy. Each patient received intravenous saline placebo in addition to conventional therapy. SETTING: Intensive care units of 10 tertiary care hospitals in North America. PATIENTS: One hundred twenty-six patients with lactic acidosis, defined as arterial blood lactate greater than or equal to 5 mmol/L and either arterial pH of less than or equal to 7.35 or base deficit greater than 6 mmol/L. Patients were followed for up to 6 months. MEASUREMENTS AND MAIN RESULTS: Mean +/- SD demographic entry data for 126 patients included: age 56 +/- 17 years, lactate 10.4 +/- 5.5 mmol/L, pH 7.24 +/- 0.14, calculated base deficit 14.1 +/- 5.4, arterial systolic blood pressure 103 +/- 29 mm Hg, Glasgow Coma score 7.9 +/- 4.9, and APACHE II score 19.2 +/- 8.1. Despite fluids and pressors, 32% of patients had systolic blood pressures of less than or equal to 90 mm Hg in association with sepsis (59%), cardiac failure (18%), or hemorrhage (18%). The most common causes of lactic acidosis in the absence of shock were sepsis (49%), liver disease (15%), and respiratory failure (12%). The median survival was 38.5 hours. Survival at 24 hours was 59%. Arterial pH predicted 24-hour survival better than base deficit or bicarbonate level. Percent survival was 41% at 3 days and 17% at 30 days. Only 21% of patients survived to leave the intensive care unit, and 17% were discharged from the hospital. In patients receiving sodium bicarbonate, neither acid-base nor hemodynamic status improved. CONCLUSIONS: In this first prospective study of the clinical course of acute lactic acidosis in adults, nearly all subjects had both hemodynamic and nonhemodynamic (metabolic) underlying causes, many of which independently predicted survival and most of which were refractory to standard care.


Subject(s)
Acidosis, Lactic/physiopathology , Acidosis, Lactic/blood , Acidosis, Lactic/complications , Acidosis, Lactic/etiology , Acidosis, Lactic/mortality , Adult , Aged , Female , Humans , Lactates/blood , Lactic Acid , Male , Middle Aged , Predictive Value of Tests , Prospective Studies , Risk Factors , Statistics as Topic , Survival Analysis
14.
Drug Metab Dispos ; 22(3): 443-6, 1994.
Article in English | MEDLINE | ID: mdl-8070322

ABSTRACT

The brain uptake and biotransformation of remacemide hydrochloride [(+/-)-2-amino-N-(1-methyl-1,2-diphenylethyl)acetamide monohydrochloride; FPL 12924AA] were studied in the rat. The brain uptake indices (BUI) for remacemide and its pharmacologically active metabolite FPL12495 [(+/-)-1-methyl-1,2-diphenylethylamine monohydrochloride] were 51 and 130%, respectively. The BUI of [14C] remacemide and [14C]FPL12495 were not affected by increasing amounts of unlabeled remacemide or FPL12495, respectively. Likewise, the BUI of remacemide was not affected by dl-amphetamine or beta-phenethylamine. A mixture of [3H]remacemide hydrochloride (3H label in the glycine moiety) and [14C]remacemide hydrochloride (14C label in 1,2-diphenyl-2-aminopropane moiety) was administered by intracarotid injection. The ratio of 14C/3H in the brain was equal to that in the injection mixture, indicating that remacemide enters the brain intact. HPLC analysis of brain extracts of rats given [14C] remacemide hydrochloride by intracarotid injection revealed that 97.8 +/- 0.2% (mean +/- SD) of the radioactivity was present as remacemide, whereas 1.9 +/- 0.2% of the radioactivity was present as FPL12495. Finally, in vitro studies revealed that remacemide is hydrolyzed by whole-brain homogenates to the pharmacologically active metabolite FPL12495. Data indicate that remacemide enters the brain by passive diffusion and undergoes deglycination at the blood-brain barrier or within the brain to give FPL12495.


Subject(s)
Acetamides/pharmacokinetics , Anticonvulsants/pharmacokinetics , Brain/metabolism , Acetamides/metabolism , Animals , Biotransformation , Carbon Radioisotopes , Male , Phenethylamines/metabolism , Phenethylamines/pharmacokinetics , Rats , Rats, Sprague-Dawley , Sulfur Radioisotopes
15.
Biopharm Drug Dispos ; 14(2): 107-18, 1993 Mar.
Article in English | MEDLINE | ID: mdl-8453021

ABSTRACT

Intravenous, sublingual, or aerosolized nitroglycerin was administered to 19 patients with coronary artery disease during clinically indicated cardiac catheterization. Eight blood samples were collected over 15 min from each patient, and analyzed for content of nitroglycerin, 1,2-glycerol dinitrate, and 1,3-glycerol dinitrate. Simultaneously, heart rate (HR), systolic blood pressure (SBP), and left ventricular end-diastolic pressure (LVEDP) were recorded. Plasma concentrations of nitroglycerin were highest after intravenous injection and lowest after sublingual tablets. Metabolite concentrations were highest after intravenous injection at early time-points; at later time-points, no between-group differences could be detected. SBP was minimally affected by intravenous nitroglycerin but was significantly reduced by sublingual and aerosolized formulations. Minor fluctuations in HR were observed in association with all three formulations. LVEDP was reduced by all three formulations of nitroglycerin but most rapidly by the intravenous form. Overall, no differences were detected in hemodynamic responses caused by sublingual and aerosolized nitroglycerin. Efficacy of sublingual and aerosolized nitroglycerin in patients undergoing cardiac catheterization is equivalent.


Subject(s)
Hemodynamics/drug effects , Nitroglycerin/pharmacology , Nitroglycerin/pharmacokinetics , Administration, Inhalation , Administration, Sublingual , Aerosols , Cardiac Catheterization/methods , Humans , Injections, Intravenous , Male , Nitroglycerin/administration & dosage , Nitroglycerin/blood
17.
J Pharm Pharmacol ; 45(1): 39-42, 1993 Jan.
Article in English | MEDLINE | ID: mdl-8094444

ABSTRACT

Hexane-water partition coefficients, pKa values, protein binding and red blood cell partitioning were studied with six phenothiazine drugs. Red cell partitioning was independent of drug concentration, and there was no correlation between partitioning and physicochemical characteristics. Red cell partitioning could be used indirectly to estimate protein binding, but two potential pitfalls of general importance were found. Failure to consider drug binding of glassware and haematocrit changes were shown to induce incorrect estimates of both red cell partitioning and protein binding, as well as hexane-water partition coefficients.


Subject(s)
Erythrocytes/metabolism , Phenothiazines/blood , Blood Proteins/metabolism , Chemical Phenomena , Chemistry, Physical , Chromatography, High Pressure Liquid , Humans , Hydrogen-Ion Concentration , Lipids/chemistry , Phenothiazines/chemistry , Protein Binding
18.
N Engl J Med ; 327(22): 1564-9, 1992 Nov 26.
Article in English | MEDLINE | ID: mdl-1435883

ABSTRACT

BACKGROUND: Mortality is very high in lactic acidosis, and there is no satisfactory treatment other than treatment of the underlying cause. Uncontrolled studies have suggested that dichloroacetate, which stimulates the oxidation of lactate to acetyl-coenzyme A and carbon dioxide, might reduce morbidity and improve survival among patients with this condition. METHODS: We conducted a placebo-controlled, randomized trial of intravenous sodium dichloroacetate therapy in 252 patients with lactic acidosis; 126 were assigned to receive dichloroacetate and 126 to receive placebo. The entry criteria included an arterial-blood lactate concentration of > or = 5.0 mmol per liter and either an arterial-blood pH of < or = 7.35 or a base deficit of > or = 6 mmol per liter. The mean (+/- SD) arterial-blood lactate concentrations before treatment were 11.6 +/- 7.0 mmol per liter in the dichloroacetate-treated patients and 10.4 +/- 5.5 mmol per liter in the placebo group, and the mean initial arterial-blood pH values were 7.24 +/- 0.12 and 7.24 +/- 0.13, respectively. Eighty-six percent of the patients required mechanical ventilation, and 74 percent required pressor agents, inotropic drugs, or both because of hypotension. RESULTS: The arterial-blood lactate concentration decreased 20 percent or more in 83 (66 percent) of the 126 patients who received dichloroacetate and 45 (36 percent) of the 126 patients who received placebo (P = 0.001). The arterial-blood pH also increased more in the dichloroacetate-treated patients (P = 0.005). The absolute magnitude of the differences was small, however, and they were not associated with improvement in hemodynamics or survival. Only 12 percent of the dichloroacetate-treated patients and 17 percent of the placebo patients survived to be discharged from the hospital. CONCLUSIONS: Dichloroacetate treatment of patients with severe lactic acidosis results in statistically significant but clinically unimportant changes in arterial-blood lactate concentrations and pH and fails to alter either hemodynamics or survival.


Subject(s)
Acidosis, Lactic/drug therapy , Dichloroacetic Acid/therapeutic use , Acidosis, Lactic/blood , Acidosis, Lactic/mortality , Arteries , Dichloroacetic Acid/administration & dosage , Female , Humans , Hydrogen-Ion Concentration , Lactates/blood , Male , Middle Aged , Survival Rate
19.
J Nutr ; 122(6): 1293-99, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1588447

ABSTRACT

Six healthy men (22-31 y) were supplemented for 4 wk with folic acid labeled with deuterium [3',5'-2H2; 3.6 mumol/d (1.6 mg/d)] to permit evaluation of in vivo kinetics of this vitamin. Total folate in urine, serum and erythrocytes was determined by microbiological assay, and isotopic labeling of urinary and erythrocyte folate was determined by gas chromatography-mass spectrometry. During supplementation, serum folate reached maximal concentration in approximately 18 d, whereas excretion of total and deuterium-labeled folates increased rapidly and reached isotopic steady state in 1-2 wk. Isotopic labeling of erythrocyte folate increased continually over the entire supplementation period. Upon cessation of supplementation, red blood cell folate and urinary folate excretion (total and labeled) decreased linearly. The decline in total serum folate could be described with a biexponential model that yielded a slow-phase half-life of 18.7 +/- 2.3 d. This model also indicated a turnover of 4.5% of the total body folate pool per day. Pool sizes of total body folate before and after supplementation (at steady state) were calculated to be 10 mumol (4.4 mg) and 98.9 mumol (43.7 mg), respectively. These kinetic data and stable isotope methodology may be used to address a wide range of experimental questions related to folate metabolism.


Subject(s)
Folic Acid/pharmacokinetics , Adult , Chromatography, High Pressure Liquid , Deuterium , Erythrocytes/metabolism , Folic Acid/blood , Folic Acid/urine , Gas Chromatography-Mass Spectrometry , Humans , Kinetics , Male
20.
Biopharm Drug Dispos ; 13(2): 141-52, 1992 Mar.
Article in English | MEDLINE | ID: mdl-1550909

ABSTRACT

Nitroglycerin was administered to eight healthy volunteers in the form of sublingual tablets, oral sustained-release tablets, and an oral solution. Blood samples were collected for measurement of nitroglycerin and its two isomeric glyceryl dinitrate metabolites. Blood pressure and pulse rate were monitored; subjective evaluations of headache, dizziness, facial flushing, skin irritation, and gastrointestinal upset were made. Nitroglycerin itself was virtually undetectable after the solution and tablet preparations; the metabolites were consistently detectable from a few minutes after dosing to 24 h later. Mean total (nitroglycerin plus metabolite) concentrations were comparable in the 15 min following sublingual administration, and the 8 h following tablet administration. The relative bioavailability of the tablets in comparison with the oral solution was 70 per cent based on metabolite concentrations. Nitroglycerin sustained-release tablets appear to exert their beneficial effects in the prolonged prophylaxis of angina through active metabolites.


Subject(s)
Nitroglycerin/analogs & derivatives , Nitroglycerin/pharmacokinetics , Administration, Oral , Administration, Sublingual , Adult , Biological Availability , Delayed-Action Preparations , Half-Life , Humans , Male , Nitroglycerin/administration & dosage , Nitroglycerin/metabolism , Tablets
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