Abundant triatomines in Texas dog kennel environments: Triatomine collections, infection with Trypanosoma cruzi, and blood feeding hosts.

Triatomine insects are vectors of the protozoan parasite Trypanosoma cruzi- the causative agent of Chagas disease. Chagas disease is endemic to Latin America and the southern United States and can cause severe cardiac damage in infected mammals, ranging from chronic disease to sudden death. Identifying interactions among triatomines, T. cruzi discrete typing units (DTUs), and blood feeding hosts is necessary to understand parasite transmission dynamics and effectively protect animal and human health. Through manual insect trapping efforts, kennel staff collections, and with the help of a trained scent detection dog, we collected triatomines from 10 multi-dog kennels across central and south Texas over a one-year period (2018-2019) and tested a subset to determine their T. cruzi infection status and identify the primary bloodmeal hosts. We collected 550 triatomines, including Triatoma gerstaeckeri (n = 515), Triatoma lecticularia (n = 15), Triatoma sanguisuga (n = 6), and Triatoma indictiva (n = 2), with an additional 10 nymphs and 2 adults unable to be identified to species. The trained dog collected 42 triatomines, including nymphs, from areas not previously considered vector habitat by the kennel owners. Using qPCR, we found a T. cruzi infection prevalence of 47 % (74/157), with T. lecticularia individuals more likely to be infected with T. cruzi than other species. Infected insects harbored two T. cruzi discrete typing units: TcI (64 %), TcIV (23 %), and mixed TcI/TcIV infections (13 %). Bloodmeal host identification was successful in 50/149 triatomines, revealing the majority (74 %) fed on a dog (Canis lupus), with other host species including humans (Homo sapiens), raccoons (Procyon lotor), chickens (Gallus gallus), wild pig (Sus scrofa), black vulture (Coragyps atratus), cat (Felis catus), and curve-billed thrasher (Toxostoma curviostre). Given the frequency of interactions between dogs and infected triatomines in these kennel environments, dogs may be an apt target for future vector control and T. cruzi intervention efforts.

Trypanosoma cruzi discrete typing unit TcIV implicated in a case of acute disseminated canine Chagas disease.

In 2006, Nabity et al. reported on an atypical presentation of Trypanosoma cruzi (T. cruzi) infection in an 8-month old English Mastiff from central Texas. Clinical signs and laboratory findings included lymphadenopathy, weight loss, amastigotes in lymph node aspirates, and initial serological results suggestive of either T. cruzi or Leishmania infection. Given the poor prognosis, the dog was euthanized and subsequent testing and culture of parasites from a lymph node revealed T. cruzi infection. Because different parasite discrete typing units (DTUs) are potentially associated with different disease outcomes in a variety of mammalian hosts, an understanding of these relationships in naturally infected dogs may be useful for informing canine prognosis and may also have human health implications. Here, we compared strains using culture versus culture-independent methods. We subjected archived cultured parasites harvested from the lymph node in the infected Mastiff to two independent approaches for determining parasite DTU, including sequencing of the TcSC5D gene and use of DTU-specific qPCR probes to hybridize the nuclear spliced leader intergenic region (SL-IR). Both approaches revealed T. cruzi discrete typing unit TcIV. Testing of multiple other tissues directly without culturing, including heart/tongue, intestine, trachea/lymph nodes, and uterus/ovary, provided further evidence of disseminated TcIV infection in this dog. We report T. cruzi DTU TcIV as the cause of a severe disseminated infection in a dog from an area with triatomine vectors in central Texas, adding to the limited body of clinicopathologic data that links specific parasite strains to disease outcomes in dogs in the US. Future studies to type parasites from asymptomatic dogs and those with diverse disease manifestations will be useful in informing the degree to which parasite genetics is associated with disease presentation and severity. If applied to antemortem samples, diagnostic typing of parasites from infected dogs may assist in determining prognosis.