Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters










Publication year range
1.
Mol Phylogenet Evol ; 111: 158-168, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28390910

ABSTRACT

High throughput sequencing technologies have revolutionized the potential to reconcile incongruence between gene and species trees, and numerous approaches have been developed to take advantage of these advances. Genotyping-by-sequencing is becoming a regular tool for gathering phylogenetic data, yet comprehensive evaluations of phylogenetic methods using these data are sparse. Here we use multiple phylogenetic and population genetic methods for genotyping-by-sequencing data to assess species relationships in a group of forest insect pests, the spruce budworm (Choristoneura fumiferana) species complex. With few exceptions, all methods agree on the same relationships, most notably placing C. pinus as basal to the remainder of the group, rather than C. fumiferana as previously suggested. We found strong support for the monophyly of C. pinus, C. fumiferana, and C. retiniana, but more ambiguous relationships and signatures of introgression in a clade of western lineages, including C. carnana, C. lambertiana, C. occidentalis occidentalis, C. occidentalis biennis, and C. orae. This represents the most taxonomically comprehensive genomic treatment of the spruce budworm species group, which is further supported by the broad agreement among multiple methodologies.


Subject(s)
Genome, Insect , Moths/genetics , Phylogeny , Polymorphism, Single Nucleotide/genetics , Animals , Discriminant Analysis , Genetic Speciation , Genetics, Population , Genotype , Geography , North America , Principal Component Analysis , Sequence Analysis, DNA , Species Specificity , United States
2.
J Insect Physiol ; 56(10): 1427-35, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20043914

ABSTRACT

Proteins that are capable of binding chitin play essential roles in the synthesis and structural integrity of the insect cuticle and peritrophic matrix. In the course of developing expressed sequence tag (EST) libraries for the eastern spruce budworm, Choristoneura fumiferana, we identified an abundant cDNA encoding a homolog of the Drosophila "gasp" gene (Gene Analogous to Small Peritrophins). For the present work, we undertook the characterization of this new homolog, CfGasp, in an effort to identify its role during larval development. As shown for DmGasp, the C. fumiferana homolog was found to contain three type-2 chitin-binding domains (CBDs), which were also found in Gasp orthologs retrieved from GenBank. In a phylogenetic analysis, these Gasp proteins formed a tight cluster, distinct from the midgut-specific peritrophins with which they share the cysteine-containing CBDs so far considered absent from cuticular proteins. However, unlike what has been shown for peritrophins, CfGasp transcript levels were low in larval midguts and most abundant in epidermis, while they were low in trachea and ovaries. Transcript levels increased during larval molts in a pattern similar to that observed for exocuticular proteins in other insects. In addition, the recombinant protein was shown to be capable of binding chitin. Altogether, these results suggest a structural role for CfGasp in exocuticle formation.


Subject(s)
Cloning, Molecular , Insect Proteins/genetics , Insect Proteins/metabolism , Moths/growth & development , Moths/genetics , Amino Acid Sequence , Animals , Base Sequence , Gene Expression Regulation, Developmental , Insect Proteins/chemistry , Larva/chemistry , Larva/genetics , Larva/growth & development , Larva/metabolism , Molecular Sequence Data , Moths/classification , Moths/metabolism , Phylogeny , Protein Structure, Tertiary , Sequence Alignment
3.
Comp Biochem Physiol B Biochem Mol Biol ; 154(2): 165-73, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19524698

ABSTRACT

Four cDNAs (Cfserpin-1a, Cfserpin-1b, Cfserpin-1c and Cfserpin-1d) of the Choristoneura fumiferana serpin-1 gene were cloned from an epidermis cDNA library. Analysis of the deduced amino acid sequences indicated that the cloned cDNAs encode four different proteins displaying identical N- but distinct C-termini, the latter region containing the inhibitory loop. The entire CfSerpin-1 gene is transcribed while the variants are generated. Antibodies generated against the purified recombinant serpins cross-reacted with the other three. Each of the four Cfserpin-1 cDNA variants was transcribed throughout larval development, from the 4th to the 6th instar, but transcript levels during the intermolt phases were generally higher than during the molting phase. The epidermis and fat body had higher levels of Cfserpin-1 transcripts than the midgut. Cfserpin-1 proteins, detected with the Cfserpin-1a antibody, were found in the epidermis, midgut, fat body, plasma and molting fluid of 6th instar larvae and pre-pupae. Prepupal and pupal insects had higher levels of the proteins than the 6th instar feeding larvae, despite a drop in transcript levels. Cfserpin-1a could bind with the serine proteinase elastase and form a complex in vitro. We hypothesize that the cloned serpins could be involved in the regulation of cuticle degradation during the insect molting cycle.


Subject(s)
DNA, Complementary/genetics , Gene Expression Profiling , Lepidoptera/enzymology , Lepidoptera/genetics , Mutation , Serpins/genetics , Serpins/metabolism , Amino Acid Sequence , Animals , Blood Coagulation , Cloning, Molecular , Genes, Insect/genetics , Immunity, Innate , Lepidoptera/growth & development , Lepidoptera/immunology , Melanins/metabolism , Molecular Sequence Data , Molting , Pancreatic Elastase/metabolism , Peptide Hydrolases/metabolism , Phylogeny , Protein Isoforms/biosynthesis , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sequence Alignment , Serpins/biosynthesis , Serpins/chemistry
4.
Arch Insect Biochem Physiol ; 61(4): 209-19, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16552767

ABSTRACT

RNA helicases play important roles in cellular processes such as pre-mRNA splicing, rRNA processing, ribosomal biogenesis, and translation. A full-length DEAD box RNA helicase cDNA (CfrHlc113) was isolated from the spruce budworm, Choristoneura fumiferana. CfrHlc113 contained the eight functional motifs, which are highly conserved in the DEAD box RNA helicase family, and an arginine-serine-aspartate (RSD) domain at its N-terminal end. CfrHlc113 was highly homologous to Rattus norvegicus HEL117 and human prp5 genes, both of which are suggested to be involved in RNA splicing. The results of Northern and Western blotting showed that expression of the CfrHlc113 gene was low or undetectable in eggs, larvae, pupae, and adults. High levels of expression were, however, detected in the three in vitro cultured cell lines, CF-203, CF-124T, and CF-70, which were developed from the midgut, ovaries, and neonate larvae, respectively. Immunocytochemistry revealed that CfrHlc113 protein was present exclusively in the nuclei of these cell lines.


Subject(s)
Lepidoptera/enzymology , RNA Helicases/genetics , Amino Acid Motifs , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Blotting, Western , Cloning, Molecular , Female , Fluorescent Antibody Technique , Lepidoptera/genetics , Molecular Sequence Data , Phylogeny , RNA Helicases/chemistry , RNA, Messenger/chemistry , RNA, Messenger/genetics , Recombinant Proteins/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment
5.
J Insect Physiol ; 51(1): 55-60, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15686646

ABSTRACT

We compared the effects of Manduca sexta allatotropin (Manse-AT) on the rate of in vitro juvenile hormone (JH) biosynthesis by the corpora allata (CA) of different-aged virgin females from migrant (Quebec) and non-migrant (Azores) populations of the armyworm, Pseudaletia unipuncta, as well as from early- and late-calling lines selected from the Quebec population. There was a significant age x strain interaction, with the observed rates of JH biosynthesis in early adult life closely reflecting strain-specific differences in the age at onset of calling. In considering data for all ages combined, treatment of CA with Manse-AT resulted in a significant increase in the rate of JH biosynthesis for all but the Late strain, although significant differences for this strain were detected at certain ages. The CA of females from the Azores strain showed the strongest stimulation, with those of 0- and 1-day-old individuals displaying a singularly high degree of sensitivity. Selection for early- and late-calling lines resulted in significant differences in the temporal patterns of JH biosynthesis but did not markedly affect the sensitivity of the CA to Manse-AT. These findings are discussed within the context of the age-related differences observed in the rates of in vitro JH biosynthesis and JH haemolymph titers previously reported in comparisons of the Quebec and Azorean strains of the true armyworm.


Subject(s)
Corpora Allata/metabolism , Insect Hormones/physiology , Juvenile Hormones/biosynthesis , Moths/metabolism , Neuropeptides/physiology , Animal Migration/physiology , Animals , Azores , Female , Manduca , Moths/genetics , Quebec , Species Specificity , Time Factors
6.
J Insect Physiol ; 49(5): 501-11, 2003 May.
Article in English | MEDLINE | ID: mdl-12770629

ABSTRACT

We report on the cloning and sequencing of two Tranosema rostrale ichnovirus (TrIV) genes, and assess their relatedness to TrV1, the gene encoding the most abundant TrIV transcript in last-instar Choristoneura fumiferana larvae parasitized by T. rostrale. One of the two newly isolated genes, TrV2, features an organization similar to that of TrV1, with one intron flanked by two exons; it encodes a 102 amino acid protein showing 79% similarity to TrV1. The third gene, TrV4, encodes a larger protein (143 aa) displaying similarity to the other two only over the first approximately 50 amino acid residues of its sequence; the remaining portion contains an imperfect octad repeat. Although the TrV4 gene contains only one exon, it has an intron similar in size and sequence to that of TrV1 and TrV2; in fact, the non-coding regions of all three genes show higher sequence identity than the coding regions, pointing to their common origin. Southern analysis suggests that each gene maps to a different TrIV genome segment, with homologous sequences apparently present on other segments. TrV1 and TrV4 transcription in penultimate (5th) instar hosts, parasitized shortly after the molt, was strong for both genes 1 and 2 days p.p., with transcript abundance decreasing after the final molt; thus, neither of these genes is upregulated during induction of developmental arrest in last-instar hosts. Cf-124T cells inoculated with T. rostrale calyx fluid showed significant levels of apoptosis 24-72 h p.i.; TrV1 was detected in the culture medium, suggesting that this and/or other TrIV-encoded proteins may be responsible for the observed cytopathic effect. Southern and Northern analyses, using DNA and RNA extracted from infected Cf-124T cells, revealed the presence of both TrV1- and TrV4-carrying genome segments and transcripts, but neither DNA, at least in episomal form, nor mRNA persisted for more than a few days p.i. This in vitro system may provide a suitable starting point for the study of TrIV gene functions.


Subject(s)
Genes, Viral , Moths/virology , Polydnaviridae/genetics , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Blotting, Southern , Blotting, Western , Cell Line , DNA Primers , DNA, Viral , Molecular Sequence Data , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid
7.
Virology ; 300(2): 316-31, 2002 Sep 01.
Article in English | MEDLINE | ID: mdl-12350362

ABSTRACT

In Campoletis sonorensis Ichnovirus (CsIV), the repeat element genes constitute a gene family of 28 members. In the present work, we document the presence of members of this gene family in two additional ichnoviruses, Hyposoter didymator Ichnovirus (HdIV) and Tranosema rostrale Ichnovirus (TrIV). Two repeat element genes, representing at least one functional gene, were identified in TrIV, whereas HdIV was found to contain at least three such genes. In both HdIV and TrIV, the known repeat element genes are encoded on single genome segments, with hybridization studies suggesting the presence of other, related but as yet uncharacterized genes. The HdIV and TrIV repeat element genes are all transcribed in infected caterpillars, although differences exist among genes in levels and in tissue specificity of expression. A heuristic tree was generated indicating that the repeat element genes are more similar within a species of wasp than between species, with TrIV genes being more closely related to the CsIV than to the HdIV genes. These results suggest that the most significant duplication, divergence, and expansion of the repeat element genes occurred after speciation. The finding that repeat element genes form an interspecific family within the genus Ichnovirus supports the view that the proteins they encode play an important role in ichnovirus biology.


Subject(s)
Genes, Viral , Polydnaviridae/genetics , Repetitive Sequences, Nucleic Acid , Wasps/virology , Amino Acid Sequence , Animals , Base Sequence , Conserved Sequence , Gene Expression Regulation, Viral , Molecular Sequence Data , Phylogeny , Polydnaviridae/classification , Polymorphism, Genetic , Transcription, Genetic
8.
Peptides ; 23(4): 645-51, 2002 Apr.
Article in English | MEDLINE | ID: mdl-11897383

ABSTRACT

Manduca sexta allatotropin (Manse-AT), a peptide originally isolated on the basis of its ability to stimulate juvenile hormone (JH) biosynthesis in the tobacco hornworm, is a potent in vitro stimulator of the corpora allata (CA) in Pseudaletia unipuncta (Lepidoptera: Noctuidae). At 10(-6)M, Manse-AT stimulated in vitro rates of JH biosynthesis by CA of day 0 and 6 adult females 15- and 10-fold respectively. Both Manse-AT and serotonin were also shown to be dose-dependent stimulators of heart rate in day 0, 3 and 6 adult males and females. Furthermore, analysis suggests that there are differences in both resting and Manse-AT-stimulated heart rates depending on age and rearing conditions.


Subject(s)
Corpora Allata/drug effects , Insect Hormones/pharmacology , Manduca/chemistry , Moths/metabolism , Neuropeptides/pharmacology , Animals , Dose-Response Relationship, Drug , Female , Heart/drug effects , Heart/physiology , In Vitro Techniques , Juvenile Hormones/biosynthesis , Male , Moths/anatomy & histology , Serotonin/pharmacology
9.
Arch Sex Behav ; 30(5): 521-33, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11501299

ABSTRACT

The victim-choice polymorphia of 178 sexual aggressors divided into six subtypes, incest offenders, pseudoincest offenders, sexual aggressors of familiar children, sexual aggressors of unfamiliar children, sexual aggressors of familiar women, and sexual aggressors of unfamiliar women, was compared. Results showed that sex offenders remained stable in their choice of victim from one offence to another in terms of victim age, victim gender, and aggressor-victim relationship. Subjects characterised by high levels of polymorphia were pseudoincest offenders and sexual aggressors of familiar women, whereas sexual aggressors of both unfamiliar women and unfamiliar children were characterised by low levels of polymorphia. Recommendations regarding how to further refine sex offender typologies are discussed.


Subject(s)
Choice Behavior , Crime Victims/classification , Criminal Psychology/classification , Sex Offenses/classification , Adolescent , Adult , Child , Child Abuse, Sexual , Crime Victims/statistics & numerical data , Criminal Psychology/statistics & numerical data , Female , Humans , Incest , Male , Prisoners/classification , Prisoners/psychology , Quebec/epidemiology , Rape , Sex Offenses/psychology , Sex Offenses/statistics & numerical data
10.
Peptides ; 22(2): 255-61, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11179819

ABSTRACT

In the sphinghid moth Manduca sexta, two allatoactive neuropeptides appear to be responsible for regulating juvenile hormone (JH) production by the corpora allata (CA). These peptides (M. sexta allatostatin, Mas-AS, and M. sexta allatotropin, Mas-AT) respectively inhibit and stimulate in vitro JH biosynthesis by CA in this insect. However, although Mas-AS inhibits CA in both larval and adult insects, Mas-AT is active only in adult M. sexta. The situation in other lepidopteran species is less clear-cut and, although both peptides have been detected (usually by immunologic and/or molecular techniques) in several other moths (including noctuids), their function as regulators of JH production remains uncertain. In the tomato moth Lacanobia oleracea (Lepidoptera: Noctuidae), we have previously demonstrated the occurrence of Mas-AS and/or Mas-AT in extracts of CA, brain and other organs, and have shown that both peptides are present in larval and adult forms. However, in L. oleracea, although Mas-AS inhibits larval and adult CA in vitro, it does so only at relatively high concentrations, and to a maximum of only approximately 70%. By contrast, Mas-AT (which is also present in larval and adult L. oleracea) stimulates larval and adult CA, but is substantially more potent ( approximately 100 fold) than the allatostatin. In this paper we present the results of paired, concurrent measurements (using ELISA) of levels of Mas-AS and Mas-AT in brains, CA and hemolymph (plasma and hemocytes) of L. oleracea at times when there are marked changes in JH titers. We also present data on the in vitro rates of JH biosynthesis by isolated CA, and on hemolymph JH esterase activity measured at the same critical developmental times, and discuss all of these data in relation to the putative allatoregulatory roles of the M. sexta allatotropic and allatostatic neuropeptides in L. oleracea.


Subject(s)
Insect Hormones/metabolism , Juvenile Hormones/biosynthesis , Manduca/metabolism , Neuropeptides/metabolism , Animals
11.
Insect Biochem Mol Biol ; 30(8-9): 855-62, 2000.
Article in English | MEDLINE | ID: mdl-10876130

ABSTRACT

Outbreaks of the black army cutworm, Actebia fennica, are associated with recently burned sites, where larvae feed on early successional plants. In the present paper we show that smoke volatiles stimulate juvenile hormone biosynthesis in virgin females, resulting in a more rapid rate of oocyte maturation and a significant advance in the age of first calling (the release of the sex pheromone) compared to control females. The ecological implications of this physiological effect are discussed.


Subject(s)
Juvenile Hormones/biosynthesis , Moths/metabolism , Smoke , Animals , Corpora Allata/metabolism , Corpora Allata/physiology , Female , Moths/physiology , Oocytes/physiology
12.
J Gen Virol ; 81(Pt 7): 1871-80, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10859395

ABSTRACT

The endoparasitic wasp Tranosema rostrale (Ichneumonidae) transmits a polydnavirus (PDV) to its host, Choristoneura fumiferana, during oviposition. Unlike most other PDVs examined, the virus of T. rostrale (TrPDV) does not appear to play an important role in suppressing the host cellular immune response. However, it inhibits host metamorphosis. In the present study, TrPDV gene expression was examined in parasitized and virus-injected last-instar caterpillars. Northern analysis with viral DNA as a probe revealed only one detectable mRNA, of about 650 bp. The corresponding cDNA, termed TrV1, was cloned and sequenced and found to encode a protein of 103 amino acids which, following cleavage of the putative signal peptide, has a predicted molecular mass of 9.3 kDa. This protein displays limited similarity to the VHv1.4 cysteine-rich protein from the PDV of Campoletis sonorensis, mostly within the signal peptide region. By using a TrV1-specific probe, the TrV1 gene was localized to segment G of the TrPDV genome. The cuticle and fat body were identified as the principal sites of TrV1 transcription, with little transcription observed in haemocytes and midgut. Western analysis of proteins extracted from selected tissues of parasitized insects suggested that the TrV1 protein is secreted in the haemolymph. As observed for other PDVs, injection of TrPDV did not suppress transcription of the gene that encodes juvenile hormone esterase, the activity of which is inhibited by the virus. We speculate that the TrV1 protein may play a role in the inhibition of C. fumiferana metamorphosis.


Subject(s)
Insecta/virology , Polydnaviridae/genetics , Wasps/virology , Amino Acid Sequence , Animals , Base Sequence , Blotting, Southern , Cloning, Molecular , Female , Gene Library , Insecta/physiology , Larva/virology , Metamorphosis, Biological , Molecular Sequence Data , Transcription, Genetic , Viral Proteins/physiology
13.
Gen Comp Endocrinol ; 117(3): 343-54, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10764546

ABSTRACT

The parasitic wasp Tranosema rostrale transmits a polydnavirus (PDV) to its host, Choristoneura fumiferana, during oviposition. Last-instar C. fumiferana larvae parasitized by T. rostrale early in the stadium fail to undergo metamorphosis, and injection of the wasp's calyx fluid (CxF; contains PDV) into healthy caterpillars induces a dose-dependent delay in initiation of metamorphosis (D. Doucet and M. Cusson, 1996, Entomol. Exp. Appl. 81, 21-30). In the present work, parasitization and injection of CxF (0.5 female equivalent) on the first day of the last stadium both prevented the rise in hemolymph 20-hydroxyecdysone (20HE) titer observed between day 4 and day 7 in control and saline-injected larvae. Similarly, juvenile hormone esterase (JHE) activity was depressed following parasitization or CxF injection, whereas control larvae displayed a peak on day 4. However, neither parasitism nor injection of CxF on day 1 prevented the JH-producing glands from turning off during the first half of the last stadium. Likewise, low but clearly detectable JH titers were observed in the first hours following the molt but very low titers, at or near the detection limit of our radioimmunoassay, were seen in both control and parasitized larvae on day 4. Prothoracic glands showed no apparent sign of degeneration 4 days after injection of CxF but had significantly smaller cells than saline-injected larvae 7 days postinjection. It is not clear whether this was a direct effect of T. rostrale PDV. Thus, disruption of spruce budworm metamorphosis by T. rostrale CxF involves depression of 20HE titers but is not associated with a measurable increase in the level of JH, as shown for some other host-parasitoid systems. In view of the latter observation, we put forward three hypotheses regarding the functional significance of the observed suppression of JHE activity in developmentally arrested C. fumiferana larvae.


Subject(s)
Carboxylic Ester Hydrolases/metabolism , Lepidoptera/enzymology , Lepidoptera/growth & development , Animals , Female , Larva/growth & development , Lepidoptera/virology , Metamorphosis, Biological , Polydnaviridae , Wasps/virology
14.
J Insect Physiol ; 45(6): 515-523, 1999 Jun.
Article in English | MEDLINE | ID: mdl-12770336

ABSTRACT

We assessed the effects of age and mating status on in vitro juvenile hormone (JH) biosynthesis, oocyte growth, egg production and vitellogenin (Vg) accumulation in the tortricid moths, Choristoneura fumiferana and C. rosaceana. To determine whether vitellogenesis is dependent on the presence of JH, we also examined the effects of decapitation and JH analog treatments on egg production. In both species, the corpora allata (CA) of adult females released fmol quantities of JH, with JH II being the major homolog produced. The CA began producing detectable quantities of JH around the time of emergence. Full activation of the CA was observed a few hours sooner in C. fumiferana than in C. rosaceana. In pharate adults and young virgin females of both species, growth of the basal oocyte reflected changes in CA activity. Decapitation of newly emerged females significantly reduced egg production, but treatment of decapitated females with the JH analog methoprene resulted in egg production that was similar to (C. fumiferana) or greater than (C. rosaceana) that of controls, indicating that JH is required for oocyte maturation. Vg was first observed in the hemolymph before the presumptive time of CA activation, suggesting that the synthesis of this protein is not dependent on JH. The presence of normal quantities of Vg in the hemolymph of pupae decapitated before CA activation confirmed this hypothesis. The Vg titer underwent a transient decline following CA activation and was significantly lower in mated than in virgin females of both species 3 and 5 days after copulation. Since CA activation at emergence and mating are both expected to cause a rise in the JH titer, we suggest that the declines in the levels of Vg result from JH-enhanced Vg uptake by the developing oocytes. Mating induced a significant increase in egg production but had no measurable impact on rates of JH biosynthesis in vitro.

15.
J Insect Physiol ; 45(7): 637-646, 1999 Jul.
Article in English | MEDLINE | ID: mdl-12770349

ABSTRACT

We used a radioimmunoassay (RIA) to assess the effect of mating on juvenile hormone (JH) titer in females of the tortricid moths Choristoneura fumiferana and C. rosaceana. Virgins had undetectable levels of JH in their hemolymph on the 5th day of the pupal stage but titers rose to 1-4 and 0.2-0.5 ng JH II eq./ml, respectively, after emergence. On days 1, 3 and 5 following copulation, females of both species had higher JH titers than virgins of the same ages, with the greatest difference between virgin and mated females observed on day 3 for C. fumiferana and on day 5 for C. rosaceana. This increase was apparently not the result of a male-to-female transfer of JH during copulation since: (i) the accessory sex glands (ASGs) of males of both species displayed a very limited ability to convert JH acid into JH, (ii) ASGs produced no JH when incubated in vitro in the presence of L-[methyl-(3)H]-methionine, (iii) ASGs of males injected with L-[methyl-(3)H]-methionine 24 h prior to dissection contained no JH-associated radioactivity, and (iv) freshly formed spermatophores dissected out of females mated to similarly injected males contained no trace of radioactive JH. In addition, the JH content of ASGs and spermatophores, as measured by RIA, was not higher than that of virgin-female hemolymph, on a per-mg basis. However, in contrast with earlier findings in other species of moths, the CA of male C. fumiferana and C. rosaceana maintained in vitro in the presence of tritiated methionine produced and released JH I, JH II and JH III in quantities and proportions similar to those reported for female glands.

16.
J Invertebr Pathol ; 72(1): 50-6, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9647701

ABSTRACT

The calyx epithelium of the campoplegine wasp, Tranosema rostrale, contains typical ichneumonid polydnaviruses (PVs) that display an apparently uncommon association with the egg chorion. The latter structure features fine hair-like projections, longest around the egg's apices. In the lumen of the ovary, T. rostrale virus becomes lodged between these projections and forms a particulate coat around the egg. In the host, Choristoneura fumiferana, projections and associated virions are observed in close contact with basement membranes of fat body and muscle tissues, to which the eggs rapidly become attached following introduction into the host hemocoel. We discuss the implications of this unusual virus-chorion association in terms of immune protection, delivery of virus to specific host tissues, and the evolution of PVs.


Subject(s)
Polydnaviridae/isolation & purification , Wasps/virology , Animals , DNA, Viral/isolation & purification , Electrophoresis, Agar Gel , Female , Moths/parasitology , Ovary , Ovum , Polydnaviridae/classification , Polydnaviridae/genetics , Polydnaviridae/ultrastructure , Virion
17.
Anal Biochem ; 249(1): 83-7, 1997 Jun 15.
Article in English | MEDLINE | ID: mdl-9193712

ABSTRACT

Optically pure (> 99.5%) enantiomers of insect juvenile hormones (JH) I, II, and III were obtained by injection of racemic mixtures onto a chiral HPLC column using hexane:2-propanol (99.5:0.5) as the mobile phase. The enantiomers of JH III were the best resolved (R = 4.26), followed by those of JH II (R = 2.29) and JH I (R = 1.47). These purified natural and unnatural enantiomers were used to further characterize an antiserum (444) developed for JH radioimmunoassays (RIAs). Based on ED50 values generated using optically pure [methyl-3H]-10R,11S-JH II as a tracer, the natural isomers of JH I, JH II, and JH III were 30, 87, and 36 times more immunoreactive, respectively, than the unnatural isomers. When compared with the racemates, the natural isomers were approximately twice as immunoreactive. In competitive displacement studies where the natural enantiomers of the three JHs were compared, immunoreactivities were in the order JH II > JH I > JH III (ED50 = 109, 198, and 300, respectively). Availability of pure natural enantiomers of JH, both as tracers and competitors, should improve the sensitivity and accuracy of JH titer determinations made by RIA and facilitate various enzyme, binding protein, and receptor studies.


Subject(s)
Antibodies, Monoclonal/isolation & purification , Chromatography, High Pressure Liquid , Juvenile Hormones/isolation & purification , Radioimmunoassay , Binding, Competitive , Logistic Models , Reference Standards , Sesquiterpenes/isolation & purification , Stereoisomerism , Titrimetry
19.
Insect Biochem Mol Biol ; 26(8-9): 767-73, 1996.
Article in English | MEDLINE | ID: mdl-9014326

ABSTRACT

A 15-residue neuropeptide, Manduca sexta allatostatin (Mas-AST), strongly inhibits juvenile hormone (JH) biosynthesis in vitro by corpora allata (CA) from Manduca fifth-stadium larvae and adult females as well as Helicoverpa zea adult females (Kramer et al., 1991 Proc. Natl. Acad. Sci (USA) 88, 9458-9462). In contrast, this study found that 1.0 microM Mas-AST has no JH biosynthesis inhibitory activity in Pseudaletia unipuncta sixth instar larvae or newly-emerged (day 0) adults but inhibited CA of 5-day-old adult females by 60%. From a P. unipuncta brain cDNA library, was isolated a cDNA that encodes a 125 amino acid polypeptide containing the Mas-AST sequence. Within the precursor, Mas-AST is situated at the carboxy terminus and is flanked by different dibasic proteolytic cleavage signals. The Pseudaletia gene specifying the Mas-AST peptide is present as a single copy per haploid genome. Expression of this gene was low in Pseudaletia sixth instar larvae, prepupae and early pupae but was relatively high in late pupae, and day 1 and 3 adults of both sexes. In day 5 adults, the relative transcript level appears to be maintained in females but declines in males. This pattern of Mas-AST expression does not correlate well with the profile of JH biosynthesis in Pseudaletia, which increases during the first 5 days of adult life, suggesting additional or alternative functions for this peptide.


Subject(s)
Insect Proteins , Moths/genetics , Peptides/genetics , Amino Acid Sequence , Animals , Base Sequence , DNA, Complementary , Female , Gene Expression , Juvenile Hormones/antagonists & inhibitors , Juvenile Hormones/biosynthesis , Male , Manduca/metabolism , Molecular Sequence Data , Neuropeptides/genetics , Protein Precursors/genetics , Pyrrolidonecarboxylic Acid/analogs & derivatives , RNA, Messenger
20.
Mol Cell Endocrinol ; 89(1-2): 121-5, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1338720

ABSTRACT

In an effort to identify the signal transduction mechanism associated with the inhibition of juvenile hormone (JH) biosynthesis by the neuropeptides allatostatins, levels of the cyclic nucleotides cAMP and cGMP were measured in corpora allata (CA) of virgin and mated Diploptera punctata females using radioimmunoassays. Treatment of isolated CA with varying concentrations of synthetic allatostatins 1, 2, 3 or 4 did not elicit significant changes in the levels of either cAMP or cGMP in any of the test glands, suggesting that these compounds do not act as second messengers for the four allatostatins tested. Simultaneous treatment of CA with allatostatin 4 and the adenylate cyclase activator forskolin did not increase the degree of inhibition of juvenile hormone biosynthesis relative to that obtained with forskolin (5 or 50 microM) alone. We interpret these results as lending further support to the suggestion that cyclic nucleotides do not play a role in the signal transduction of allatostatins 1-4 in cockroach CA.


Subject(s)
Cockroaches/physiology , Cyclic AMP/physiology , Cyclic GMP/physiology , Juvenile Hormones/biosynthesis , Neuropeptides/pharmacology , Second Messenger Systems/drug effects , Animals , Cockroaches/drug effects , Colforsin/pharmacology , Corpora Allata/drug effects , Corpora Allata/metabolism , Cyclic AMP/analysis , Cyclic GMP/analysis , Depression, Chemical , Female , Sexual Behavior, Animal
SELECTION OF CITATIONS
SEARCH DETAIL
...