ABSTRACT
BACKGROUND AND OBJECTIVES: Depth of invasion and stage of the disease are established prognostic indicators in cutaneous malignant melanoma. The role of other parameters is still an open problem. METHODS: In 93 consecutive patients with cutaneous malignant melanoma, the level of invasion, tumor thickness, ulceration, vascular invasion, lymphoplasmocytic infiltrates, and mitotic index were evaluated by histology. Expression of Ki-67 and PCNA proliferative antigens together with vimentin, S100, and HMB 45 proteins were assessed by immunohistochemistry. RESULTS AND CONCLUSIONS: Disease-free and overall survival were correlated with tumor stage, tumor thickness, level of invasion, macroscopic pattern, ulceration, vascular invasion, expression of HMB 45, PCNA, and Ki-67/MIB1. Stage, HMB 45, and PCNA were independent prognostic factors for disease-free survival, whereas tumor stage, tumor thickness, and expression of both proliferative antigens influenced overall survival independently. The variables studied demonstrated reciprocal correlation; therefore, analysis of many prognostic parameters in malignant melanoma could be recommended.
Subject(s)
Melanoma/pathology , Skin Neoplasms/pathology , Adolescent , Adult , Aged , Analysis of Variance , Antigens, Neoplasm , Cell Division , Chemotherapy, Adjuvant , Dermatologic Surgical Procedures , Disease-Free Survival , Female , Humans , Immunohistochemistry , Ki-67 Antigen/analysis , Lymph Node Excision , Male , Melanoma/metabolism , Melanoma/therapy , Melanoma-Specific Antigens , Middle Aged , Neoplasm Invasiveness , Neoplasm Proteins/analysis , Prognosis , Proliferating Cell Nuclear Antigen/analysis , Proportional Hazards Models , Skin Neoplasms/metabolism , Skin Neoplasms/therapy , Vimentin/analysisABSTRACT
The concentration of N-nitrosamines was determined in the gastric juice of 170 patients between 6 months and 33 years after operation for ulcer disease, including 38 patients after resection with the Rydygier method (BI-R), 36 after Billroth II method (BII), 35 after truncal vagotomy with pyloroplasty (VP), 41 after highly selective vagotomy (WWW) and 20 after gastroenterostomy (ZZJ). The concentration of N-nitrosamines in the gastric juice was estimated chromatographically. It was confirmed that their concentration in the stomach was the highest after ZZJ and BII resection, lowest after WWW.
Subject(s)
Gastric Juice/chemistry , Nitrosamines/analysis , Stomach Ulcer/metabolism , Stomach Ulcer/surgery , Adult , Aged , Female , Gastroenterostomy , Humans , Male , Middle Aged , Postoperative Period , VagotomyABSTRACT
In a group of 96 patients with peptic ulcer (36 patients with ulcer of the stomach, 60 patients with ulcer of the duodenum) we analysed the alterations of the mucosa of the stomach associated with the ulcer. A significantly frequent occurrence was shown of the athropic gastritis and of the dysplasia in patients with ulcer of the stomach in comparison with the group of patients with ulcer of the stomach in the comparison with the group of the patients with ulcer of the duodenum. Among the patients analysed we also appraised the alterations on the picture of the stomach mucosa of the Helicobacter pylori infection, of the duodeno-gastric reflux as well as of the concentration of the bile acids and of lysolecithin contained in the gastric juice in the ulcerous disease of the stomach and of the duodenum. The presence of the Helicobacter pylori infection was identified bacteriologically, the duodeno-gastric reflux was diagnosed with the radionucleide method and the concentration of the bile acids and lysolecithin in gastric juice was determined chromatographically. It was confirmed that the Helicobacter pylori infection as well as the bile acids and the lysolecithin the concentration of which in the gastric juice in patients with an ulcer in the stomach were significantly higher in comparison with patients with and the ulcer in the duodenum, were a decisive factor for more exacerbated histological alterations of the mucosa of the stomach in case of ulcer of the stomach.
Subject(s)
Duodenal Ulcer/pathology , Duodenogastric Reflux/complications , Gastric Mucosa/pathology , Helicobacter Infections/complications , Helicobacter pylori/isolation & purification , Stomach Ulcer/pathology , Adult , Atrophy , Bile Acids and Salts/chemistry , Duodenal Ulcer/etiology , Female , Gastric Juice/chemistry , Humans , Lysophosphatidylcholines/analysis , Male , Middle Aged , Stomach Ulcer/etiologyABSTRACT
Among 108 persons sick from chronic gastritis, 61 with ulcus ventriculi and 91 with ulcus doudeni, diagnostic of Helicobacter pylori with simultaneous utilization of the following methods: direct preparation of homogenous tissue (gastrobiopsy), histological preparation (coloring by Giemza method), culture and serologic test (ELISA and IFP) was carried out. It was proved that among sick persons under examination depending on the diagnostic methods used, frequency of the infection was as follows 20%--direct preparation, 63.8%--histological preparation, 68.1%--culture, 81.5%--serologic test. Differences in prevalence of H. pylori in respective sickness depending on diagnostic methods were noted. It was also determined on the ground of discrimination analysis that in order to obtain the best diagnostic value the most advisable is to couple serologic test and histological examination.
Subject(s)
Duodenal Ulcer/microbiology , Gastritis/microbiology , Helicobacter Infections/microbiology , Helicobacter pylori/isolation & purification , Stomach Ulcer/microbiology , Adult , Aged , Chi-Square Distribution , Female , Humans , Male , Middle Aged , Serologic Tests/methodsABSTRACT
Among 184 sick persons with chronic gastritis proved histologicaly, frequency of appearance of duodenal gastric reflux was estimated scintigraphically, and frequency of appearance of IgG against Helicobacter pylori by serologic test (ELISA and IFP). Reflux was proved in 70 (38%) cases, whereas in 152 (82.6%) specific IgG was found. Using GLM program in the statistic analysis it was found that impact of each of these factors on inflammatory changed gastric mucous membrane is different. The weakest impact of reflex was on appearance of GChA in stomach, impact was 1.9 times higher on appearance of GChpA, GChSimp.--6.4 times higher and of GChSup. as high as 23.9 times. Whereas the weakest impact of Helicobacter pylori was on appearance of GChSimp. in stomach, in appearance of GChSup. the impact was 1.4 times higher, GChpA--1.9 times higher, while GChA 2.1 times higher. Taking into consideration interaction between reflux and infection it was proved that bacterial factor (Helicobacter pylori) acts on gastric mucous membrane (except chronic superficial inflammation) from 2.3 to 30.1(!) times higher (depending on form of chronic inflammation) than duodenal-gastric reflux.
