ABSTRACT
Traumatic acid (TA) is a plant hormone (cytokinin) that in terms of chemical structure belongs to the group of fatty acids derivatives. It was isolated from Phaseolus vulgaris. TA activity and its influence on human cells and organism has not previously been the subject of research. The aim of this study was to examine the effects of TA on collagen content and basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione, thiol group content, and lipid peroxidation in physiological conditions. The results show a stimulatory effect of TA on tested parameters. TA caused a decrease in membrane phospholipid peroxidation and exhibited protective properties against ROS production. It also increases protein and collagen biosynthesis and its secretion into the culture medium. The present findings reveal that TA exhibits multiple and complex activity in fibroblast cells in vitro. TA, with its activity similar to unsaturated fatty acids, shows antioxidant and stimulatory effects on collagen biosynthesis. It is a potentially powerful agent with applications in the treatment of many skin diseases connected with oxidative stress and collagen biosynthesis disorders.
Subject(s)
Collagen/biosynthesis , Dicarboxylic Acids/pharmacology , Fibroblasts/drug effects , Oxidative Stress/drug effects , Skin/cytology , Cell Membrane/metabolism , Cells, Cultured , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Lipid Peroxidation/drug effects , Phospholipids/metabolism , Skin/drug effectsABSTRACT
N6-benzyladenine and kinetin are adenine-type cytokinins that play various roles in many aspects of plant development and stimulate anabolic processes in plant cells. The aim of this study was to examine the effect of N6-benzyladenine and kinetin on basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione and thiol group content, and lipid peroxidation. The results show a stimulatory effect of kinetin and N6-benzyladenine on antioxidative enzyme activity, as well as reduced glutathione and thiol group content. Cytokinins caused a decrease in membrane phospholipid peroxidation and exhibited protective properties against malondialdehyde production. The present findings reveal that both N6-benzyladenine and kinetin exhibit multiple and complex actions in fibroblast cells in vitro. Both show antioxidant properties and are potentially powerful agents with applications in the prevention and treatment of many diseases connected with oxidative stress in skin, for example, psoriasis.
