ABSTRACT
The goal of our study was to assess suitability of FNA biopsy material as a source of samples (cell suspension) for DNA ploidy assessment in neoplastic tumors using flow cytometry. DNA ploidy is an established prognostic factor in many types of cancers. Aneuploid breast tumors are characterized by increased aggressiveness which manifests itself through rapid local progression and metastatic spread. Investigated specimens were breast cancer FNA biopsy cell suspensions. Measurements were performed using flow cytometry. Material studied comprised 143 cases analyzed in 1999-2000. We found in this group 101 carcinoma cases with aneuploid type and 42 cases of primary breast carcinoma with diploid type of cell cycle. Immunocytochemical assesssment of estrogen receptor and progesterone receptor status was performed in group of 105 cases. DNA ploidy was compared to receptor status of the investigated cells. DNA aneuploidy correlated with weak or no reaction for the presence of estrogen and progesterone receptors. Our study demonstrates the suitability of DNA ploidy assessment method applied to cytological material from FNA biopsies.
Subject(s)
Breast Neoplasms/genetics , Carcinoma/genetics , DNA/genetics , Adult , Aged , Aged, 80 and over , Aneuploidy , Biopsy, Needle , Breast Neoplasms/pathology , Carcinoma/pathology , Disease Progression , Female , Flow Cytometry , Humans , Immunohistochemistry , Middle Aged , Ploidies , Prognosis , Receptors, Estrogen/genetics , Receptors, Estrogen/metabolism , Receptors, Progesterone/genetics , Receptors, Progesterone/metabolismABSTRACT
The aim of present study was to investigate the effect of free beta cells transplantation on carbohydrate tolerance in rats with streptozotocin-induced diabetes. Free beta cells were isolated by mechanical disruption of isolated pancreatic islets in Ca and Mg free medium. 1500 and 3000 isolated islets were used for the isolation of 500 x 10(3)-1.099 x 10(3) and 1.398 x 10(3)-2.098 x 10(3) free cells, respectively. Between 78% and 98% of all isolated cells were the variable cells. After isogenic transplantation of free beta cells, blood glucose concentration in all animals was normalized within 14 days. The rate of glycemia normalization was related to the amount of viable free cells. The glucose assimilation coefficient after intravenous glucose administration in normal rats, diabetic rats and diabetic rats after transplantation of free cells was: 2.98 x 10(-2); 0.68 x 10(-2) and 2.04 x 10(-2) mg/dl/min, respectively. Allogenic transplantation of free beta cells caused only a transient decrease in blood glucose level.
