Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Language
Publication year range
1.
J Bacteriol ; 190(1): 264-74, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17981982

ABSTRACT

Biofilms are structured communities characterized by distinctive gene expression patterns and profound physiological changes compared to those of planktonic cultures. Here, we show that many gram-negative bacterial biofilms secrete high levels of a small-molecular-weight compound, which inhibits the growth of only Escherichia coli K-12 and a rare few other natural isolates. We demonstrate both genetically and biochemically that this molecule is the amino acid valine, and we provide evidence that valine production within biofilms results from metabolic changes occurring within high-density biofilm communities when carbon sources are not limiting. This finding identifies a natural environment in which bacteria can encounter high amounts of valine, and we propose that in-biofilm valine secretion may be the long-sought reason for widespread but unexplained valine resistance found in most enterobacteria. Our results experimentally validate the postulated production of metabolites that is characteristic of the conditions associated with some biofilm environments. The identification of such molecules may lead to new approaches for biofilm monitoring and control.


Subject(s)
Bacteria/genetics , Biofilms , Escherichia coli K12/genetics , Plasmids , Valine/analysis , Amino Acids/analysis , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , DNA, Bacterial/drug effects , DNA, Bacterial/genetics , Escherichia coli K12/drug effects , Escherichia coli K12/growth & development , Kinetics , Valine/genetics , Valine/metabolism
2.
J Bacteriol ; 189(23): 8746-9, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17905994

ABSTRACT

Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.


Subject(s)
Culture Media/chemistry , Culture Media/pharmacology , Escherichia coli/drug effects , Escherichia coli/physiology , Amino Acids/chemistry , Amino Acids/metabolism , Carbohydrates/chemistry , Carbon/metabolism , Cell Proliferation/drug effects , Fermentation
3.
J Bacteriol ; 189(18): 6512-20, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17586646

ABSTRACT

Growing bacterial L forms are reputed to lack peptidoglycan, although cell division is normally inseparable from septal peptidoglycan synthesis. To explore which cell division functions L forms use, we established a protocol for quantitatively converting a culture of a wild-type Escherichia coli K-12 strain overnight to a growing L-form-like state by use of the beta-lactam cefsulodin, a specific inhibitor of penicillin-binding proteins (PBPs) 1A and 1B. In rich hypertonic medium containing cefsulodin, all cells are spherical and osmosensitive, like classical L forms. Surprisingly, however, mutant studies showed that colony formation requires d-glutamate, diaminopimelate, and MurA activity, all of which are specific to peptidoglycan synthesis. High-performance liquid chromatography analysis confirmed that these L-form-like cells contain peptidoglycan, with 7% of the normal amount. Moreover, the beta-lactam piperacillin, a specific inhibitor of the cell division protein PBP 3, rapidly blocks the cell division of these L-form-like cells. Similarly, penicillin-induced L-form-like cells, which grow only within the agar layers of rich hypertonic plates, also require d-glutamate, diaminopimelate, and MurA activity. These results strongly suggest that cefsulodin- and penicillin-induced L-form-like cells of E. coli-and possibly all L forms-have residual peptidoglycan synthesis which is essential for their growth, probably being required for cell division.


Subject(s)
Escherichia coli/growth & development , L Forms/growth & development , Peptidoglycan/biosynthesis , Anti-Bacterial Agents/pharmacology , Cefsulodin/pharmacology , Cell Division , Cell Wall/chemistry , Culture Media , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli/metabolism , L Forms/drug effects , Mutation , Piperacillin/pharmacology , beta-Lactams/pharmacology
4.
J Biol Chem ; 281(22): 15238-48, 2006 Jun 02.
Article in English | MEDLINE | ID: mdl-16597620

ABSTRACT

The Escherichia coli proteins DksA, GreA, and GreB are all structural homologs that bind the secondary channel of RNA polymerase (RNAP) but are thought to act at different levels of transcription. DksA, with its co-factor ppGpp, inhibits rrnB P1 transcription initiation, whereas GreA and GreB activate RNAP to cleave back-tracked RNA during elongational pausing. Here, in vivo and in vitro evidence reveals antagonistic regulation of rrnB P1 transcription initiation by Gre factors (particularly GreA) and DksA; GreA activates and DksA inhibits. DksA inhibition is epistatic to GreA activation. Both modes of regulation are ppGpp-independent in vivo but DksA inhibition requires ppGpp in vitro. Kinetic experiments and studies of rrnB P1-RNA polymerase complexes suggest that GreA mediates conformational changes at an initiation step in the absence of NTP substrates, even before DksA acts. GreA effects on rrnB P1 open complex conformation reveal a new feature of GreA distinct from its general function in elongation. Our findings support the idea that a balance of the interactions between the three secondary channel-binding proteins and RNAP can provide a new mode for regulating transcription.


Subject(s)
Escherichia coli Proteins/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , RNA, Bacterial/genetics , RNA, Bacterial/metabolism , RNA, Ribosomal/genetics , RNA, Ribosomal/metabolism , Transcription Factors/metabolism , rRNA Operon , Base Sequence , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Bacterial/metabolism , DNA-Directed RNA Polymerases/chemistry , DNA-Directed RNA Polymerases/metabolism , Epistasis, Genetic , Escherichia coli Proteins/genetics , Kinetics , Models, Biological , Multiprotein Complexes , Promoter Regions, Genetic , Transcription Factors/genetics , Transcription, Genetic
5.
Mol Microbiol ; 56(4): 958-70, 2005 May.
Article in English | MEDLINE | ID: mdl-15853883

ABSTRACT

In Escherichia coli the beta-lactam mecillinam specifically inhibits penicillin-binding protein 2 (PBP2), a peptidoglycan transpeptidase essential for maintaining rod shape. We have previously shown that PBP2 inactivation results in a cell division block and that an increased concentration of the nucleotide ppGpp, effector of the RelA-dependent stringent response, confers mecillinam resistance and allows cells to divide as spheres in the absence of PBP2 activity. In this study we have characterized an insertion mutation which confers mecillinam resistance in wild-type and DeltarelA strains but not in DeltarelADeltaspoT strains, devoid of ppGpp. The mutant has an insertion in the fes gene, coding for enterochelin esterase. This cytoplasmic enzyme hydrolyses enterochelin-Fe(3+) complexes, making the scavenged iron available to the cells. We show that inactivation of the fes gene causes iron limitation on rich medium plates and a parallel SpoT-dependent increase of the ppGpp pool, as judged by the induction of the iron-regulated fiu::lacZ fusion and the repression of the stringently controlled P1(rrnB)::lacZ fusion respectively. We further show, by direct ppGpp assays, that iron starvation in liquid medium produces a SpoT-dependent increase of the ppGpp pool, strongly suggesting a role for iron in the balance of the two activities of SpoT, synthesis and hydrolysis of (p)ppGpp. Finally, we present evidence that ppGpp exerts direct or indirect positive control on iron uptake, suggesting a simple homeostatic regulatory circuit: iron limitation leads to an increased ppGpp pool, which increases the expression of iron uptake genes, thereby alleviating the limitation.


Subject(s)
Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Guanosine Tetraphosphate/metabolism , Iron/metabolism , Penicillin-Binding Proteins/metabolism , Pyrophosphatases/metabolism , Amdinocillin/metabolism , Anti-Bacterial Agents/metabolism , Carboxylic Ester Hydrolases/genetics , Carboxylic Ester Hydrolases/metabolism , Culture Media/chemistry , Drug Resistance, Bacterial , Enterobactin/metabolism , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Gene Expression Regulation, Bacterial , Penicillin-Binding Proteins/genetics , Pyrophosphatases/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism
6.
C R Biol ; 326(2): 215-7, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12754939

ABSTRACT

A scheme is presented whereby a new genetic control circuit can be introduced into an organism, permitting the experimenter to turn the expression of a given gene (or set of genes) on or off at will. The proposed scheme involves a positive feedback loop--here, a positive regulator, the CII protein of phage lambda, with its structural gene engineered so as to require CII for its expression. This feedback loop creates the possibility of two stable steady states, with gene cII ON or OFF. Genes added downstream of cII and lacking a promoter will follow the same expression as cII. Two additional circuits allow the experimenter to switch at will between the ON and OFF states.


Subject(s)
DNA/genetics , Gene Expression Regulation , Genetic Engineering/methods , Bacteriophage lambda/genetics , Escherichia coli/genetics , Escherichia coli/virology , Feedback, Physiological , Genes, Synthetic , Genes, Viral , Promoter Regions, Genetic , Transcription Factors/genetics , Transcription Factors/physiology , Viral Proteins , Viral Structural Proteins/genetics
7.
Res Microbiol ; 153(7): 425-6, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12405348

ABSTRACT

Seeing connections between apparently unrelated areas is the hallmark of a deep thinker. Maurice Hofnung showed that his interest in the maltose regulon and my own interest in the regulation of cell division in Escherichia coli could lead to fruitful collaboration. From the maltose regulon to the LamB receptor to phage A to the SOS response to the Mutatest to induction of expression of the SOS-inducible division inhibitor SfiA to the SOS Chromotest based on sfiA::lacZ induction to the development of a commercial kit for measuring the genotoxicity of environmental substances...this was but one of the original trails that Maurice Hofnung blazed and exploited successfully.


Subject(s)
Escherichia coli/cytology , Escherichia coli/genetics , Maltose/metabolism , Cell Division , Colorimetry , Escherichia coli/metabolism , History, 20th Century , Mutagenicity Tests , SOS Response, Genetics
8.
Bioessays ; 24(6): 512-8, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12111734

ABSTRACT

Whenever the state of a biological system is not determined solely by present conditions but depends on its past history, we can say that the system has memory. Bacteria and bacteriophage use a variety of memory mechanisms, some of which seem to convey adaptive value. A genetic type of heritable memory is the programmed inversion of specific DNA sequences, which causes switching between alternative patterns of gene expression. Heritable memory can also be based on epigenetic circuits, in which a system with two possible steady states is locked in one or the other state by a positive feedback loop. Epigenetic states have been observed in a variety of cellular processes, and are maintained by diverse mechanisms. Some of these involve alternative DNA methylation patterns that are stably transmitted to daughter molecules and can affect DNA-protein interactions (e.g., gene transcription). Other mechanisms exploit autocatalytic loops whereby proteins establish the proper conditions for their continued synthesis. Template polymers other than nucleic acids (e.g., components of the cell wall) may also propagate epigenetic states. Non-heritable memory is exemplified by parasitic organisms that bear a signature of their previous host, such as host-controlled modification of phage DNA or porin hitchhiking in predatory bacteria. The heterogeneous nature of the examples known may be indicative of widespread occurrence of memory mechanisms in bacteria and phage. However, the actual extent, variety and potential selective value of prokaryotic memory devices remain open questions, still to be addressed experimentally.


Subject(s)
Bacteria/genetics , Bacteriophages/genetics , DNA/genetics , Bacteriophages/metabolism , Bacteriophages/physiology , Circadian Rhythm , DNA Methylation , Lac Operon , Models, Genetic , Temperature
SELECTION OF CITATIONS
SEARCH DETAIL
...