ABSTRACT
Contractile responses of airways segments of porpoises inhaling nanopowder CoFe2O4 were stidued by means of a mechanographic method. Inhalation of the nanosize particles of CoFe2O4 in vivo and in vitro testing the nanomaterial on isolated smooth muscles led to potentiation histaminergic, cholinergic contractile activity in airways of porpoises and to strengthening of adrenergic relaxing answers. Nanosize particles vary amplitude of hyperpotassium reductions in smooth muscle segments of airways similarly to the effect of depolymerizing drug colchicine.
Subject(s)
Bronchi , Cobalt/adverse effects , Ferric Compounds/adverse effects , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Nanoparticles/adverse effects , Animals , Bronchi/drug effects , Cobalt/pharmacology , Ferric Compounds/pharmacology , Guinea Pigs , Male , Muscle Contraction/physiology , Muscle, Smooth/physiology , Nanoparticles/administration & dosage , Trachea/drug effects , Trachea/physiologyABSTRACT
Influence of exogenous nitroso-glutatyon on intensity of oxidizing processes in smooth muscles of colon and bronchial tubes in intact and atopic sensitised porpoises (guinea pigs) was studied. In sensitised porpoises, antioxidant protection has been initially reduced against the background of increased maintenance of products of oxidizing that reflects a picture of oxidizing damage and can be associated with an inflammatory process. In incubation with nitroso-glutatyon, a decrease in activities of syperoxiddismutase and catalase is marked and, in sensitised animals, this effect has been expressed to a lesser degree. Syperoxiddismutase and catalase are antioxidant for the enzymes participating in protection of cells from free-radical damage. A dose-dependence decrease in activity catalase and syperoxiddismutase is defined by a parity of the enzymes participating in disintegration of nitrosoglutatyon and the enzymes which have kept antioxidant activity.
Subject(s)
Catalase/metabolism , Myocytes, Smooth Muscle/drug effects , Nitric Oxide Donors/pharmacology , Oxidative Stress , S-Nitrosoglutathione/pharmacology , Superoxide Dismutase/metabolism , Animals , Catalase/analysis , Guinea Pigs , Myocytes, Smooth Muscle/enzymology , Superoxide Dismutase/analysisABSTRACT
Features of the pharmacological sensitivity of smooth muscles in the walls of arteries of the lesser circulation circle in rabbits with respect to cholinergic, histaminergic, and adrenergic agents, as well as the influence of endothelium on the realization of contractile response to these agents have been investigated in rabbits. A special feature in the cholinergic relaxation of smooth muscles of a pulmonary artery is a two-componential character of the dose dependence. The low-threshold component of the relaxant action of pilocarpine exhibits the endothelium-dependent character. An important feature of the contractile reaction to histaminergic action is a direct contractile effect of histamine, which is not observed in vessels of the greater circulation circle. Endothelium produces an inhibiting effect on the histaminergic contractility. The basic feature in the response to adrenergic action is the beta-adrenergic contractility effect. Activation of the cAMP-dependent alarm system in smooth muscles of the pulmonary arteries leads to a constrictive effect. The established features of the pharmacological sensitivity of smooth muscles of the artery walls of the lesser circulation circle are important for the clinical pharmacology.
Subject(s)
Muscle, Smooth, Vascular/drug effects , Neurotransmitter Agents/pharmacology , Pulmonary Artery/drug effects , Animals , Muscle Contraction/drug effects , Pulmonary Circulation/drug effects , Rabbits , Vasoconstriction , VasodilationABSTRACT
Now sireos problem of pulmonology there are the diseases connected with infringement of coordinated regulation of a tone of smooth muscles of vessels and airways of ways that conducts to dissociation of parameters haemodinamyc and ventilation of lungs and as consequence, to infringement airwave-perfusion attitudes. In the review features humoral regulation contractile activity of smooth muscles of vessels of a small circle of blood circulation, a role of endocellular alarm systems in these mechanisms, and endothelium, as the local modulator endocrine functions are considered. Disgusting muscles of a small circle are distinguished from the main vessels of the big circle of blood circulation with predisposition to the raised mechanical pressure. In spite of the fact that endothelium renders modulating relaxe influence on contractile answers of smooth muscles of vessels of a venous and arterial small circle of blood circulation at action corresponding vasoconstriction, pulmonary veins are capable to endothelium-dependent dilatation to a lesser degree, in comparison with pulmonary arteries. And, on the contrary, in absence endothelium, they are characterized with high sensitivity to vasopression to substances--serotonin, histamine, phenylephrine. Features of regulation smooth muscle pressure pulmonary an artery are shown in contractile reactions of its isolated segments in reply to influence beta-adreno agonist--isoprotherenol and phosphoesterase inhibitors. Though, increase in endocellular concentration cyclic nucleotides (cAMP and\or cGMP), on the standard representations, cannot explain growth of a mechanical pressure of smooth muscles, apparently, in contractile reactions of a pulmonary artery to influence biologically and physiologically active substances interfere more complex mechanisms in which basis processes of interaction of smooth muscles cells lay, endothelium and cells of a microenvironment. Finding-out of the contribution cyclic nucleotides in these processes demands the further researches.
Subject(s)
Blood Circulation/physiology , Endothelium, Vascular/physiology , Muscle Contraction/physiology , Muscle, Smooth, Vascular/physiology , Animals , Endothelium, Vascular/metabolism , Humans , Muscle, Smooth, Vascular/metabolism , Receptors, Cell Surface/metabolism , Signal Transduction/physiologyABSTRACT
Contractile properties of smooth muscles of bronchial tubes of porpoises in formation of hyperreactance airways, were studied. Sensitization by ovalbumin results in formation of hyperresponsiveness of the airways as shown in increase in amplitude of contractile responses to histaminergic influences and oppression of adrenergic relaxations of segments. Respiratory epithelium oppresses contractile responses of airways in intact animals to histaminergic influences. Influence of epithelium on adrenergic responses depends on precontractile factor: they increase in prereduction by histamine. In formation of hyperresponse, the epithelium loses ability to modulate responses of smooth muscles of the airways. Incubation segments of smooth muscles of the airways in interleukin 5 increases contractile responses to histaminergic influences. Receptor of interleukin 5 (the soluble fraction) neutralizes the effect of exogenous interleukin 5, and incubation of receptor of sensitized animals oppresses histaminergic contractile responses.
Subject(s)
Bronchi/metabolism , Bronchial Hyperreactivity/metabolism , Histamine/pharmacology , Interleukin-5/pharmacology , Muscle Contraction/drug effects , Muscle, Smooth/metabolism , Animals , Bronchi/pathology , Bronchial Hyperreactivity/pathology , Guinea Pigs , Histamine/metabolism , Interleukin-5/metabolism , Male , Organ Culture Techniques , Respiratory Mucosa/metabolism , Respiratory Mucosa/pathologyABSTRACT
Mechanography was used to study contractile reaction of airway smooth muscles in experimental bronchial asthma in intact guinea-pigs and those incubated with IL-5. Development of experimental bronchial asthma (BA) in the animals leads to enhancement of contractile reactions of bronchial smooth muscles in response to histamine action. The exposure to IL-5 of the preparations of bronchial smooth muscles increases histamine-mediated bronchial contractility. This may explain the phenomenon of IL-5-mediated bronchial hyperreactivity in the absence of eosinophilic damage to the tissues. Expression of mRNA of IL-5 receptor alpha-chain suggests that development of IL-5-mediated hypersensitivity of bronchial smooth muscles occurs due to the presence of a relevant IL-5 receptor on their surface.
Subject(s)
Bronchi/drug effects , Bronchi/pathology , Bronchial Hyperreactivity/pathology , Interleukin-5/pharmacology , Animals , Bronchial Hyperreactivity/drug therapy , Guinea Pigs , Histamine/pharmacology , In Vitro Techniques , Male , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Muscle, Smooth/physiology , RNA, Messenger/drug effects , RNA, Messenger/metabolism , Receptors, Interleukin/drug effects , Receptors, Interleukin/genetics , Receptors, Interleukin-5ABSTRACT
By mehanographic method, contraktile responses of the airways smooth muscles in experimental bronchial asthma in porpoises intact and incubated with interleykin-5, were studied. Sensitization of ovalbumin animals results in development of hypersensitivity to inhalation of the fiber with external attributes of infringement of bronchial passableness such as cough and a shortness of breath. Morphologically the process is accompanied by destruction of epithelium of the bronchial tubes, development of an immune inflammation in a bronchial wall, and a hypertrophy of the muscular layer. In the sensitized animals, development of hyper-responsiveness of the airways smooth muscles to histamine was obvious as shown in a significant decrease of the threshold concentration and an increase in the maximal amplitude of reduction. Interleukin-5 was shown to strengthen this process. Responses to holinergic and R2-adrenerdic influences practically did not change. The data obtained corroborate the hypothesis of development of the interleukin-5-depending bronchial hyper-responsiveness in absence of eosinophile damage in the mucous membrane of the bronchial tubes.
