[Diagnostic and prognostic value of interleukin-6 in malignant non-Hodgkin's lymphomas]. / Diagnosticheskoe i prognosticheskoe znachenie interleikina-6 pri zlokachestvennykh nekhodzhkinskikh limfomakh.

Sixty six patients with non-Hodgkin's lymphomas (NHL) were studied, interleukin-6 (IL-6) was revealed in the blood sera of 33 patients. IL-6 was revealed more frequently in patients with high-grade malignant (p < 0.05) than in those with low-grade malignancy. The largest group of IL-6 positive patients included NHL patients with diffuse large B-cell lymphoma and angioimmunoblastic lymphoma. The marked relationship was found between the serum IL-6 levels and the stage of disease: the serum IL-6 level was significantly lower in untreated patients with Stages II and III disease than in those with end-stage (IV) NHL. IL-6 significantly decreased upon remission, comparable with its level before the initiation of treatment. Analysing the association of prognosis of disease with the serum IL-6 showed that in the group of patients with good (The SNLG index < 2) and intermediate (2 < SNLG index < by 2.6) prognosis, the concentration of this cytokine was significantly lower than in those with poor prognosis (SNLG index > 2.6). There was a significant decrease of the total survival rates of NHL with serum IL-6 found. Therefore, IL-6 is a good prognostic marker in NHL and associated with the activity of a malignant process. Additionally, the increased serum IL-6 levels correlated with NK activities positively and with serum IL-4 levels negatively.

[Various cellular mechanisms of the action of laser irradiation of blood]. / Nekotorye kletochnye mekhanizmy deistviia lazernogo oblucheniia krovi.

Effect of in vitro exposure to therapeutic He-Ne laser at a wave-length of 630 nm in two modes, 2 and 6 mWt, on the function of blood cells is studied. Exposure to 6 mWt resulted in spontaneous increase of interleukin-1 production by peripheral blood mononuclears. 2 mWt exposure led to increase of erythrocyte deformability and decrease of their viscosity, whereas 6 mWt exposure did not modify these parameters. Laser-effected changes in the production of other cytokines, primarily interleukins 2, 6, and 8, are a perspective trend of research.

[Connection between Epstein-Barr virus infection and HLA phenotype and features of cytokine status in patients with non-Hodgkin's lymphomas]. / Sviaz' infektsii virusom Epshteina-Barr s HLA-fenotipom i osobennostiami tsitokinovogo statusa u bol'nykh so zlokachestvennymi nekhodzhkinskimi limfomami.

Thirty-four primary (untreated) patients with non-Hodgkin's lymphomas (NHL) infected with Epstein-Barr virus (EBV) were examined. Their HLA phenotype and the production of interleukin-1 beta and tumor necrosis factor alpha were assessed. Serological profiles characteristic of the late stages and reactivation of EBV infection were detected in 16 (47.1%) patients. NHL of low malignancy predominated in EBV-infected patients. A greater number of blank HLA-A antigens and a higher incidence of HLA-DR7 antigen was observed in infected patients. Serum concentration of tumor necrosis factor alpha was reliably higher in them, whereas the production of this cytokine by the peripheral blood mononuclears decreased. Hence, serum tumor necrosis factor is a product of transformed B lymphocytes. Spontaneous and stimulated production of interleukin-1 beta by peripheral blood mononuclears was significantly decreased in EBV-infected patients, and the serum concentration of this cytokine similarly had a trend to decrease, which indicates an inhibition of interleukin-1 beta production in EBV-infected patients with NHL.

[The Epstein-Barr virus in patients with infectious endocarditis]. / Virus Epshteina--Barr u bol'nykh infektsionnym éndokarditom.

Serological markers of Epstein-Barr virus (EBV) infection has been investigated in 28 patients with infectious endocarditis. In 75% of patients IgM antibodies to "early" antigen of the virus which are the marker of active viral infection occurred vs 6.2% among healthy blood donors. Specific for infectious endocarditis reaction profile (anti-EBV combination in one person) indicates reactivation of latent viral infection. The conclusion is made on the necessity of further investigation of both the role of EBV in pathogenesis of pyoseptic diseases and immunologic mechanisms for reactivation of latent viral infections.

[The activation mechanisms in cytomegalovirus infection]. / O mekhanizmakh aktivatsii tsitomegalovirusnoi infektsii.

Antibodies to cytomegalovirus class IgG and IgM and level of tumor necrosis factor (TNF) in the serum were measured in 81 patients with severe surgical infections. In patients with acute lung abscess TNF level was twice higher and activation of cytomegalovirus infection (specific IgM) was three times more frequent than in patients with infectious endocarditis. The conclusion is made that severe surgical infections inducing progressive immunodeficiency create conditions for activation of latent viral infections, cytomegalovirus, in particular). Recommendations on prevention of hemotransmissible cytomegalovirus infection are presented.

[Paramagnetic properties of hepatic tissues and transplantable hepatomas]. / Paramagnitnye svoistva tkanei pecheni i perevivaemykh gepatom

Paramagnetic properties of frozen samples of normal, fetal and regenerating liver tissues and of a number transplantable hepatomas of different level of differentiation were studied. The results obtained indicate that ESR spectrum character and the concentration of paramagnetic centres in frozen samples of live tissues as well reflect the differentiation level, and the level of tissue functional activity. It is shown of hepatoma 22 taken as an example that tumour growth is accompanied with a change of the content of free radicals and some paramagnetic centres in the tumour and hosts liver. It can be considered as a manifestation of a biophysical shift which characterizes the tumour growth and includes the system effect of the tumour on the organism.