Chronic myeloproliferative disorders in bone marrow biopsies.

This Diagnostic Seminar intends to announce that CMPDs can be classified from BMB histologically by a rather simple system, which can be applied by interested histopathologists successfully. The rationale of this classification is to stay within the groups of diseases which are outlined by clinical findings including the peripheral blood and bone marrow smears. The concept of traditional classification as given by the WHO and textbooks, however, has to be revised as follows (1) Primary diseases of CMPDs must be distinguished from advanced disorders. Primary diseases are CML, P. vera, Thrombocythemia, CMGM, and unclassifiable CMPD. (2) Idiopathic, primary myelosclerosis of the bone marrow is a reactive feature consecutive to neoplastic transformation of hematopoiesis, i.e. myeloproliferation. (3) Advanced disorders comprise (3.1.) excess of blasts and blast crisis, and (3.2.) early myelosclerosis, myelosclerosis and myelofibrosis, advanced myelofibrosis. Advanced disorders are designated by a composed term classifying them among the groups of primary disease and specifying the advanced stage by a suffix, so that the underlying disease remains coining the term, even in unclassifiable cases in which only CMPDs can be applied. (4) The CML group must be subtyped into CML of common type versus that with increase or predominance of megakaryocytes. By this system of classification, it seems possible to classify and type the spectrum of variations occurring among CMPDs to a satisfying result.

Pituitary luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin from birth to puberty in female and male rats.

Pituitary content and concentration of LH, FSH and prolactin were measured by radioimmunoassay (RIA) at 2-day intervals from birth to puberty in female and male rats. During the first 2 to 3 weeks of life all hormones were low in pituitary content and concentration in both sexes. They all increased in females during the third and fourth week, but decreased sharply during the days before vaginal opening. During the first ovulatory cycle pituitary content and concentration of LH and prolactin increased again, FSH, however, remained low. In males, pituitary LH, FSH and prolactin content reached peak levels during puberty. Our results show a distinct sexual dimorphism for pituitary FSH. Pituitary LH and prolactin content and concentration patterns show similar tendencies in both sexes with a delay of several days in males. The dramatic changes in female pituitary hormone concentrations just before the first ovulation were not detected in males before the first occurrence of mature spermatozoa in the tubuli of the testes.