ABSTRACT
We investigated the effects of caffeic acid phenethyl ester (CAPE) on cardiac damage after blunt chest injury. Forty male adult Wistar albino rats were divided into four groups; control, cardiac contusion, cardiac contusion + CAPE, and CAPE. CAPE, 10 mmol/kg, was administered intraperitoneally for 7 days following cardiac contusion. Heart tissue and blood were obtained at the end of the experimental period. Cardiac histopathology was determined using hematoxylin & eosin (H & E) staining. Expression of tumor necrosis factor-alpha (TNF-α) in cardiomyocytes was determined using immunohistochemistry. Cardiac apoptosis was determined using the TUNEL method. Serum creatine kinase (CK), creatine kinase-muscle/brain (CK-MB) and lactate dehydrogenase (LDH) levels were determined using spectrophotometric methods. The serum cardiac troponin I (C-TI) level was measured using the ELISA method. Myofibril loss was detected in the cardiomyocytes of the cardiac contusion group. Increased apoptosis and TNF-α expression were observed in the cardiac contusion group compared to the control group. Increased CK, CK-MB, LDH and C-TI levels were found in the cardiac contusion group. We found that CAPE administration improved myocardial function. Compared to the cardiac contusion group, CK, CK-MB, LDH and C-TI levels decreased significantly in the cardiac contusion + CAPE group. Administration of CAPE significantly inhibited apoptosis and cardiac TNF-α expression. Our findings demonstrate the therapeutic effects of CAPE for cardiac contusion damage after blunt chest trauma.
Subject(s)
Caffeic Acids/pharmacology , Myocardial Contusions/drug therapy , Myocardium/metabolism , Phenylethyl Alcohol/analogs & derivatives , Wounds, Nonpenetrating/drug therapy , Animals , Antioxidants/pharmacology , Apoptosis/drug effects , Heart/drug effects , Male , Phenylethyl Alcohol/pharmacology , Rats, Wistar , Thoracic Injuries/drug therapyABSTRACT
Zn, Cd, Cr, Hg, As (total), Cu, Pb, and Ni levels of the deepwater rose shrimp (Parapenaeus longirostris, Lucas 1846), which were collected from the Tekirdag coast of the Marmara Sea, were evaluated. The Marmara Sea is the recipient of discharges from both land-based sources and the Black Sea Bosphorus stream. There are large numbers of anthropogenic activities in the coastal region of the northern Marmara Sea that include urban effluent, discharges from touristic resorts, agricultural runoff, fishing, and transportation. Heavy metal contamination of water resources may cause critical health problems for the people living around these water bodies. In deepwater rose shrimp (P. longirostris), the highest concentration level detected for Zn was 22.4 ± 24.4 mg/kg in winter 2012, Cd 0.106 ± 0.01 mg/kg in summer 2012, Cr 0.77 ± 0.05 mg/kg in winter 2012, Hg 0.18 ± 0.04 mg/kg in summer 2011, As 9.93 ± 1.4 mg/kg in spring 2012, Cu 25.48 ± 0.3 mg/kg in winter 2012, Pb 2.12 ± 0.8 mg/kg in spring, and Ni 19.25 ± 7.1 mg/kg in spring. The values of heavy metal analysis were compared to both the Turkish Food Codex (TFC) limits and international standards for human consumption. The Pb, As, and Cu levels were found to be higher than the maximum allowable limits.
