[Current incidence of accidents on Austrian ski slopes]. / Aktuelles Unfallgeschehen auf österreichischen Skipisten.

BACKGROUND: Alpine skiing and snowboarding are the most popular winter sports. These sports are also associated with a certain injury risk which, however, has steadily decreased during the past decades. During the winter season 2002/2003 the last large survey on ski injuries in Austria was performed. Among others, modern skiing equipment and optimized slope preparation may impact on the injury risk. We hypothesise that these changes may have led to a further decrease in ski injuries during the past decade. METHODS: In the winter season 2012/2013, skiing injuries were recorded in 26 Austrian ski areas. Data were collected from rescue personnel on ski slopes and by physicians in the hospital or doctors practice with the help of a questionnaire. RESULTS: A total of 7325 injured skiers and snowboarders (age: 34.8 ±â€Š17.8 years) were recorded (49 % males and 51 % females; 80 % skiers, 14 % snowboarders, 6 % others). The most frequent causes of injury were self-inflicted falls (87 %) and collisions with other skiers/snowboarders (8 %). Most affected injury locations among skiers were the knee (41 %; predominantly in female skiers, > 50 %), shoulder/back (18 %) and arms (10 %). Most affected injury locations among snowboarders were arms (38 %) and shoulder/back (23 %). Head injuries were found at the same frequency (8 %) in skiers and snowboarders. The calculated injury rate was about 0.6 injuries per 1000 skier days and has decreased by more than 50 % during the past decade. CONCLUSIONS: Modern skiing equipment and optimised slope preparation may be at least partly responsible for the decreased injury risk on ski slopes which is supported by the observation of a reduced falling frequency. Future preventive measures should focus on a reduction of knee injuries in female skiers.

Effects of supervised exercise on gamma-glutamyl transferase levels in patients with isolated impaired fasting glucose and those with impaired fasting glucose plus impaired glucose tolerance.

AIM: To study the effects of a supervised exercise program on serum gamma-glutamyl transferase (GGT), glycemic control and cardiovascular risk factors in pre-diabetic patients with isolated impaired fasting glucose (IFG) and those with IFG plus impaired glucose tolerance (IGT). METHODS: Out of 60 pre-diabetic patients (30 with isolated IFG and 30 with IFG + IGT) 24 were randomly assigned to the supervised exercise program (1 h twice a week) and 36 only obtained counselling on the risk of diabetes and its prevention. Patients have been followed over a 12-month period. RESULTS: The main findings were that patients with IFG + IGT had increased GGT levels at baseline (49.2±27.4 U/L) compared to subjects with isolated IFG (28.1±21.9 U/L) (p<0.01), and that GGT levels improved only after the supervised exercise intervention within the IFG + IGT subjects ( - 17.7±19.6 U/L). Similarly, baseline triglyceride levels were also higher in IFG + IGT patients (p<0.001) and there was a decrease through exercise intervention in these patients only (p<0.05). CONCLUSION: GGT is an unspecific marker of oxidative stress and both high plasma glucose and triglycerides levels may produce oxidative stress. Thus, patients with IFG + IGT seem to have higher levels of oxidative stress than those with isolated IFG. Based on the known association between GGT levels and cardiovascular risk factors, IFG + IGT patients may be at higher risk for the development of cardiovascular diseases. The specific effect of regular exercise on GGT in pre-diabetic patients may contribute to the understanding of the preventive effects related to exercise.

Benzoylformate decarboxylase from Pseudomonas putida as stable catalyst for the synthesis of chiral 2-hydroxy ketones.

The thiamin diphosphate- and Mg2+-dependent enzyme benzoylformate decarboxylase (BFD) from Pseudomonas putida was characterized with respect to its suitability to catalyze the formation of chiral 2-hydroxy ketones in a benzoin-condensation type reaction. Carboligation constitutes a side reaction of BFD, whereas the predominant physiological task of the enzyme is the non-oxidative decarboxylation of benzoylformate. For this purpose the enzyme was obtained in sufficient purity from Pseudomonas putida cells in a one-step purification using anion-exchange chromatography. To facilitate the access to pure BFD for kinetical studies, stability investigations, and synthetical applications, the coding gene was cloned into a vector allowing the expression of a hexahistidine fusion protein. The recombinant enzyme shows distinct activity maxima for the decarboxylation and the carboligation beside a pronounced stability in a broad pH and temperature range. The enzyme accepts a wide range of donor aldehyde substrates which are ligated to acetaldehyde as an acceptor in mostly high optical purities. The enantioselectivity of the carboligation was found to be a function of the reaction temperature, the substitution pattern of the donor aldehyde and, most significantly, of the concentration of the donor aldehyde substrate. Our data are consistent with a mechanistical model based on the X-ray crystallographic data of BFD. Furthermore we present a simple way to increase the enantiomeric excess of (S)-2-hydroxy-1-phenyl-propanone from 90% to 95% by skillful choice of the reaction parameters. Enzymatic synthesis with BFD are performed best in a continuously operated enzyme membrane reactor. Thus, we have established a new enzyme tool comprising a vast applicability for stereoselective synthesis.

Stereoselective formation of Bis(alpha-hydroxy ketones) via enzymatic carboligation.

The enzymatic approach to a novel class of chiral bis(alpha-hydroxy ketones) of type 5 and 8, which enable the synthesis of new multidentate ligands for asymmetric transition metal catalysis, is described. The key step is the second benzoylformate decarboxylase catalyzed C-C-bond formation between an aromatic dialdehyde and acetaldehyde, which proceeds with complete stereocontrol. Transformation of enantiomerically enriched monoadduct (S)-4 (ee 88%) and (S)-7 (ee 79%) resulted in optical pure (S,S)-5 and (S,S)-8 besides minor amounts of the corresponding diastereomeric meso-forms.