ABSTRACT
PURPOSE: To study the effect of Salvia Miltiorrhiza Bunge on the expression of osteoprotegerin (OPG) in cultured human periodontal ligament fibroblasts. METHODS: Primary culture of human periodontal ligament fibroblasts was established and the fifth passage cells were used in this study. Concentration-dependent effect of Salvia Miltiorrhiza Bunge on OPG mRNA and OPG protein secretion were determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbant assay (ELISA), respectively. The data were analyzed with SPSS15.0 software package. RESULTS: OPG mRNA expression was increased after 1 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05). OPG protein secretion was significantly increased after 12 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05). CONCLUSION: Salvia Miltiorrhiza Bunge can up-regulate the expression of OPG in cultured human periodontal ligament fibroblasts.
Subject(s)
Osteoprotegerin , Periodontal Ligament , Fibroblasts , Humans , RANK Ligand , RNA, Messenger , Salvia miltiorrhizaABSTRACT
PURPOSE: To assess the efficacy of intraoral open reduction of mandibular body fracture using titanium miniplate rigid fixation and intermaxillary traction titanium screw to rigidly tract the jaw during the operation. METHODS: 54 mandibular fractures in 45 patients were included in this study. After general anaesthesia, the intermaxillary traction titanium screw was inserted in the alveolar bone, between the two incisors, between the canine and the first premolar, between the first molar and the second molar, and the intermaxillary traction was finished with 0.5 mm stainless steel wire. After the mandibular arch and occlusion relationship were returned to preoperative condition, transoral mucoperiosteal incision was made to expose the fracture lines and fragments. Two 2.0 mm titanium miniplates were adapted along the Champy's line of ideal osteosynthesis and secured. After operation the stainless steel wire was released. Panoramic radiograph and clinical examination were applied at the first day and three months after operation to exhibit bone union and occlusion. RESULTS: Most patients displayed satisfied occlusion and bone union without major complications. Seventeen patients had numbness of the lower lips after operation, and the symptom was disappeared three months later. Two patients had improvement of numbness of the lower lips. CONCLUSION: Intraoral open reduction of mandibular body fracture using titanium miniplate rigid fixation and intermaxillary traction titanium screw showed high efficiency in fixation of the mandibular arch fractures , which occurred in the body and the angle of the mandible.
