Biocompatibility of Ajwain Oil Combined with Eugenol and Zinc Oxide as a Deciduous Root Canal Obturating Material: An In Vivo Study.

Aim and objectives: The present study was intended to assess the biocompatibility of newly formulated materials such as zinc oxide (ZO) admixed with ajwain oil (A) as well as ajwain eugenol (E) (1:1 ratio) against ZOE through an animal model as root canal obturating materials in deciduous teeth. Materials and methods: The study involved randomly selected 24 albino rats, which were divided into three groups based on test materials. Two polyethylene tubes (PETT) (8 mm long × 1 mm internal diameter) were inserted into connective tissues of the dorsal side on either side of each rat viz empty tube (negative control) and another containing test material (test group). Animals were sacrificed at the end of the 7th and 21st days. PETT with surrounding connective tissues were excised. Histopathological evaluations of the material's biocompatibility were done by determining inflammatory tissue responses. Non-parametric tests such as Kruskal-Wallis and Mann-Whitney U were used for statistical analysis (p < 0.05). Results: Histopathological examination on the 7th day showed increased polymorphonuclear cells for all test materials compared to the negative control (p = 0.92), suggesting acute inflammation. The inflammation subsided gradually after 21 days (p = 0.48). The lymphocytes increased after 21 days for all the materials indicating chronic inflammation (p = 0.79), as well as fibroblasts (p = 0.34) and capillaries (p = 0.35), indicating healing and repair. Conclusion: The newly formulated obturating materials were found to be biocompatible compared to ZOE. How to cite this article: Dahake PT, Joshi SS, Kale YJ, et al. Biocompatibility of Ajwain Oil Combined with Eugenol and Zinc Oxide as a Deciduous Root Canal Obturating Material: An In Vivo Study. Int J Clin Pediatr Dent 2023;16(3):431-436.

Bioinductive and anti-inflammatory properties of Propolis and Biodentine on SHED.

Objectives: This study aimed to evaluate and compare the cell viability, differentiation potential and anti-inflammatory potential of propolis and Biodentine™ on stem cells isolated from human exfoliated deciduous teeth (SHED). Materials and methods: SHED were segregated and cultured from the dental pulp of children after therapeutic extraction. Microculture Tetrazolium Assay (MTT) assay was carried out for assessing cell proliferation potential of propolis and Biodentine at different concentrations. As per the results from cell proliferation assay, cell differentiation potential of SHED was evaluated at concentration of 12.5 µg/ml using Alizarin Red staining. The anti-inflammatory potential of test materials was evaluated using gelatin zymography by detecting MMP-2 and MMP-9. Results: The maximum cell proliferation percentage of SHED treated with propolis and Biodentine was observed at a concentration of 12.5 µg/ml, on day 7, 14 and 21 with Biodentine having maximum cell proliferation potential followed by propolis. SHED treated with Biodentine showed maximum cell differentiation on day 7 (107.16), 14 (106.29) and 21 (107.72). However, anti-inflammatory activity against MMP-2 was 95 % with propolis and 85 % with Biodentine and whereas, against MMP-9 it was 65 % for propolis and 47 % for Biodentine. Conclusion: Propolis shows comparable cell viability, cell proliferation and differentiation potential on SHED when compared to Biodentine. It also exhibits better invitro anti-inflammatory activity on SHED compared to Biodentine. Further studies are warranted to validate the application of propolis as an effective and economical alternative biocompatible agent to Biodentine for vital pulp therapies.

Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Triphala, Eucalyptus, and Carvacrol against Enterococcus faecalis: An In Vitro study.

Objective: This study aimed to evaluate the antimicrobial effect of sodium hypochlorite (NaOCl), Triphala, Eucalyptus, and carvacrol on Enterococcus faecalis as the most common microorganism isolated from infected root canals. Materials and methods: Seventy-five mandibular premolar teeth were randomly distributed into five study groups: 5.25% NaOCl, 10% Triphala, 1.25% Eucalyptus, 0.6% carvacrol, and negative control (saline) group. Samples were taken using paper points from the canal spaces and using Gates-Glidden (GG) drills from dentinal tubules; after sample culturing, the colony forming unit (CFU) were counted, which was analyzed by the Wilcoxon signed-rank test. Results: All irrigants have shown a reduction of microorganisms in the root canal space. After the use of NaOCl and Eucalyptus, the bacterial count was significantly reduced in the canal, as well as dentin sampling, as compared to Triphala and carvacrol. The antimicrobial efficiency of all irrigants against E. faecalis revealed a significant difference (p < 0.05). Conclusion: All irrigants exerted significant antimicrobial activity against E. faecalis. Around 1.25% of Eucalyptus was the most effective irrigant than 5.25% of NaOCl, Triphala, and carvacrol. How to cite this article: Panchal VV, Dahake PT, Kale YJ, et al. Comparative Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite, Triphala, Eucalyptus, and Carvacrol against Enterococcus faecalis: An In Vitro study. Int J Clin Pediatr Dent 2022;15(5):514-519.

Comparison of Cariostatic and Remineralizing Potential of Two Commercial Silver Diamine Fluoride Preparations using Confocal Laser Microscopy and EDX-SEM Spectroscopy: An In Vitro study.

Aim: To investigate the cariostatic and remineralizing effect of two commercial silver diamine fluoride (SDF) preparations on enamel and dentinal caries using a bacterial plaque model. Materials and methods: A total of 32 extracted primary molars were divided into two groups (n = 16), group I (FAgamin), and group II (SDF). Plaque bacterial model was used to induce caries on enamel and dentin. Preoperative evaluation of samples was done using confocal laser microscopy (CLSM) and energy-dispersive X-ray spectroscopy-scanning electron microscope (EDX-SEM). All samples were treated with test materials and evaluated for postoperative remineralization quantification. Results: Energy-dispersive X-ray spectroscopy (EDX) revealed that mean preoperative levels (in weight %) of silver (Ag) and fluoride (F2) in carious enamel lesions were 0.0 and 0.0, which increased postoperatively to 11.40 and 31.05 for FAgamin and 13.61 and 31.87 for SDF, respectively. For dentinal caries, EDX revealed mean preoperative levels (in weight %) of Ag and F2 were 0.0 and 0.0, which increased to 11.47 and 48.71 for FAgamin and 10.16 and 47.82 for SDF, respectively postoperatively. Both the groups showed evident demineralization with exposed collagen under SEM. The mean value of enamel lesion depth for the group I and II were 38.64 and 39.30 µm, that reduced to 28.02 and 28.70 µm while for dentinal caries, the mean depth from 38.05 and 38.29 µm that reduced significantly to 28.96 and 30.10 µm, respectively (p < 0.001). Caries depth declined significantly after the application of both FAgamin and SDF (p < 0.001). Conclusion: FAgamin and SDF show similar cariostatic and remineralization potential for dental caries. The bacterial plaque model used in this study is an efficient method to induce artificial carious lesions in teeth. Clinical significance: A comparative evaluation of these two cariostatic and remineralizing agents will aid in identifying the efficacy of both commercial products in treating initial caries lesions in an effective noninvasive and child-friendly manner. How to cite this article: Kale YJ, Misal S, Dadpe MV, et al. Comparison of Cariostatic and Remineralizing Potential of Two Commercial Silver Diamine Fluoride Preparations using Confocal Laser Microscopy and EDX-SEM Spectroscopy: An In Vitro study. Int J Clin Pediatr Dent 2022;15(6):643-651.

Comparative Evaluation of Cariostatic and Remineralizing Potential of Two Commercial Silver Diamine Fluoride Preparations Using Confocal Laser Microscopy and EDX-SEM Spectroscopy: An In Vitro Study.

Aim: To investigate the cariostatic and remineralizing effect of two commercial silver diamine fluoride (SDF) preparations on enamel and dentinal caries using a plaque bacterial model. Materials and methods: Thirty-two extracted primary molars were divided into two groups (n = 16) as group I (Advantage Arrest) and group II (e-SDF). Plaque bacterial model was used to induce caries on enamel and dentin. Preoperative evaluation of samples was done using confocal laser scanning microscopy (CLSM) and energy-dispersive X-ray spectroscopy-scanning electron microscope (EDX-SEM). All samples were treated with test materials and evaluated for postoperative remineralization quantification. Results: EDX revealed that mean preoperative levels (in weight%) of silver (Ag) and fluoride (F) in enamel carious lesions were 0.0 and 0.0, which increased postoperatively to 11.40 and 31.05 for Advantage Arrest and 13.61 and 31.87 for e-SDF, respectively. For dentinal caries, EDX revealed mean preoperative levels (in weight%) of Ag and F were 0.0 and 0.0, which increased to 11.47 and 48.71 for Advantage Arrest and 10.16 and 47.82 for e-SDF, respectively postoperatively. Both groups showed evident demineralization with exposed collagen under SEM. The mean values of enamel lesion depth for the group I and II were 38.64 and 39.30 µm, that reduced to 28.02 and 28.70 µm, while for dentinal caries, the mean depth from 38.05 to 38.29 µm that reduced significantly to 28.96 and 30.10 µm, respectively (p < 0.001). Caries depth declined significantly after the application of both Advantage Arrest and e-SDF (p < 0.001). Conclusion: Advantage arrest and e-SDF show similar cariostatic and remineralization potential for dental caries. The plaque bacterial model used in this study is an efficient method to induce artificial carious lesions in teeth. How to cite this article: Kale YJ, Misal S, Dadpe M, et al. Comparative Evaluation of Cariostatic and Remineralizing Potential of Two Commercial Silver Diamine Fluoride Preparations Using Confocal Laser Microscopy and EDX-SEM Spectroscopy: An In Vitro Study. Int J Clin Pediatr Dent 2022;15(4):442-449.

Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials - An in vitro experimental study.

INTRODUCTION: Stem cells have unmatched capacity and potential for regeneration and when used alone or in combination with scaffolds to replace or repair damaged cells, can differentiate into any mature cell. AIM: To evaluate the functional differentiation potential of EMD (Enamel Matrix Derivative), MTA (Mineral Trioxide Aggregate) and Biodentine on Stem Cells from Human Exfoliated Deciduous teeth (SHED). OBJECTIVE: To determine functional differentiation potential (osteogenic/odontogenic) of various biomaterials on SHED. MATERIAL AND METHOD: SHED derived from 5th linear passage after sub-culturing were treated with EMD, MTA and Biodentine individually and their effect on cell viability was compared and evaluated by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) assay for 7 days. Alizarin red S staining was used to assess mineralization potential of these materials by the staining calcium deposits for 14 days. The results were analyzed using One-way ANOVA, Post hoc Tukey's test for multiple comparisons. RESULTS: It was observed that EMD imparted the highest cell viability at the end of 7 days (p < 0.001) followed by Biodentine and MTA. Likewise EMD showed highest potential to enhanced mineralization and expression of dentine sialoprotein (p < 0.001) followed by Biodentine and MTA at the end of 14 days (p<0.001). CONCLUSION: It can be concluded that all the tested materials are bioinductive to SHED. EMD can be used for various vital pulp therapies as that of Biodentine and MTA with predictable as well as enhanced success rate.

Effect of different pediatric drug formulations on color stability of composite, zirconia-reinforced glass ionomer cement, and glass ionomer cement.

INTRODUCTION: Various liquid drug formulations are prescribed to the children. Frequent use of these drugs can result in staining of dental restorations, affecting their longevity and durability. AIM: This study aims to evaluate the effect of different pediatric drug formulations on color stability of various esthetic restorative materials. MATERIALS AND METHODS: Three different restorative materials namely composite resin, Zirconomer Improved, and conventional glass ionomer cement (GIC) were used to prepare total 120 (40 from each) disc-shaped specimens having dimensions 12 mm × 1.5 mm. The specimens of each material were divided into five experimental groups (n = 8) through stratified random sampling technique and were immersed in five different liquid medications: Group 1 - amoxicillin + clavulanic acid, Group 2 - metronidazole, Group 3 - cephalexin, Group 4 - ibuprofen, and Group 5 - ibuprofen + paracetamol. All samples were agitated for 2 min and cycle was repeated eight hourly for 1 week with intermittent pH cycling to simulate conditions similar to the oral environment. Color stability of all specimens was evaluated using spectrophotometer. One-way ANOVA and Tukey's post hoc HSD test were used for statistical analysis. RESULTS: Results obtained revealed that Δ E* (color difference) elevation was significantly low with GIC (P < 0.001) and high with composite for all five groups. Amoxicillin + clavulanic acid and metronidazole group showed highest color stainability among all groups. CONCLUSION: GIC showed better color stability with all drug formulations. The highest color alteration was observed in composite resin, whereas zirconia reinforced GIC showed color stability lesser than GIC but better than composite.

In vitro evaluation of antimicrobial property of silver nanoparticles and chlorhexidine against five different oral pathogenic bacteria.

INTRODUCTION: Numerous antimicrobial agents are used to eliminate oral biofilm. However due to emergence of multi drug resistant microorganisms, the quest to find out biologically safe and naturally available antimicrobial agents continues. AIM: To evaluate antimicrobial efficacy of silver nano-particles against five common oral pathogenic bacteria. OBJECTIVE: To determine antimicrobial activity of silver nanoparticles and chlorhexidine gluconate against oral pathogenic bacteria. MATERIAL AND METHOD: We used strains of Streptococcus mutans (MTCC 497), Streptococcus oralis (MTCC 2696), Lactobacillus acidophilus (MTCC 10307), Lactobacillus fermentum (MTCC 903), and Candida albicans (MTCC 183). We used commercially available silver nanoparticles (experimental group) and chlorhexidine gluconate (positive control). We determined minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) of both agents and analyzed the data using paired 't' test, one way ANOVA and Tucky's post Hoc HSD. RESULT: Silver nanoparticles inhibited bacterial growth moderately. The mean MIC of AgNP against S. mutans was 60 ±â€¯22.36 µg/ml, S. oralis - 45 ±â€¯11.18 µg/ml, L. acidophilus - 15 ±â€¯5.59 µg/ml, L. fermentum - 90 ±â€¯22.36 µg/ml, Candida albicans - 2.82 ±â€¯0.68 µg/ml respectively. For chlorhexidine gluconate, mean MIC for S. mutans was 300 ±â€¯111.80 µg/ml, S. oralis - 150 ±â€¯55.90 µg/ml, L. acidophilus - 450 ±â€¯111.80 µg/ml, L. fermentum - 450 ±â€¯111.80 µg/ml and Candida albicans - 150 ±â€¯55.90 µg/ml. MIC and MBC values of AgNP were significantly lower than chlorhexidine gluconate and statistically significant (p < 0.05). CONCLUSION: Silver nanoparticles exhibited better bacteriostatic and bactericidal effect with concentration less than five folds as compared to chlorhexidine. Silver nanoparticles when used in appropriate concentration as safe alternative to present chemically derived other antimicrobial agents.

Evaluation of antimicrobial efficacy of Trachyspermum ammi (Ajwain) oil and chlorhexidine against oral bacteria: An in vitro study.

INTRODUCTION: Plaque removal is of utmost importance for control of dental caries and other associated diseases of oral cavity. However, various natural agents have proven their efficacy over chemotherapeutic agents in terms of antibacterial activity against various microorganisms. The effect is mainly due to polyphenol as its major constituent. AIM: In this in vitro study, we aimed to determine the antibacterial efficacy of Trachyspermum ammi oil at different concentrations against five oral bacteria. HYPOTHESIS: Herbal compound, T. ammi oil is effective in reducing five oral plaque-forming bacteria. MATERIALS AND METHODS: We determined the antimicrobial activity of T. ammi oil (test material) against chlorhexidine (gold standard). Pure cultures of Streptococcus mutans MTCC No 497, Streptococcus oralis MTCC No. 2696, Lactobacillus acidophilus MTCC No. 10307, Lactobacillus fermentum MTCC No. 903, and Candida albicans MTCC No. 183 were obtained and grown in selective culture media. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of both materials were evaluated by serial dilution and disc diffusion method, respectively. RESULTS: Our results revealed that T. ammi oil moderately inhibits bacterial growth with mean MIC of 250, 125, 250, 125, and 250 µg/ml, respectively. Mean MBC for T. ammi oil obtained was 18.60 ± 0.65, 11.60 ± 1.14, 14.10 ± 0.55, 11.50 ± 0.61, and 15.10 ± 0.74 mm. The MIC and MBC values were higher as compared to chlorhexidine gluconate and it was statistically significant. CONCLUSION: T. ammi (ajwain) can serve as a potential, natural, nontoxic, and economical therapeutic antiplaque agent.

Management of a massive compound odontoma in a 9-year child.

The purpose of this paper is to describe the case of surgical management of massive compound odontoma with 36 denticles in a 9-year-boy who presented with a complaint of pain and swelling in mandibular left posterior region and retained 74. The denticles were removed after the removal of retained 74 completely and wound healing was observed. Odontomas are considered as hamartomatous dental malformation rather than true neoplasm of odontogenic origin. They are the most commonly occurring abnormally formed dental tissues that interfere with eruption of associated teeth. The eruption disturbances seen due to odontomas are delayed eruption or deflection of associated teeth. These malformations are usually asymptomatic and discovered during routine radiographic investigations. Correct diagnosis followed by proper treatment plan results in a favorable prognosis.

Dental health and treatment needs among children in a tribal community.

OBJECTIVE: To assess the dental health status and treatment needs among children of 'Pardhi' tribal community. METHODS: A total of 185 children were examined over a period of 2 months using WHO proforma. The statistical software namely SPSS version 15.0 and data was analyzed using Student's t-test and ANOVA test at p < 0.05. RESULTS: The mean score for dft and DMFT was 1.87 ± 1.073 and 2.04 ± 1.564 respectively with males subjects were having comparatively more scores. It was also found significant differences between age groups. Most of the children needed one surface filling, i.e. 29.40%, followed by pulp care and restoration (19.30%), two or more surface fillings (15.60%) and extraction (11.70%). CLINICAL SIGNIFICANCE: The study subjects were characterized by a lack of dental care services, high prevalence of dental caries and treatment needs. Therefore, implementation of a basic oral health care program for this tribal population is a high priority.