ABSTRACT
INTRODUCTION: Super-paramagnetic iron oxide nanoparticles (SPIONs) are known as promising theranostic nano-drug carriers with magnetic resonance imaging (MRI) properties. Applying the herbaceous components with cytotoxic effects as cargos can suggest a new approach in the field of cancer-therapy. In this study mesoporous silica coated SPIONs (mSiO2@SPIONs) containing curcumin (CUR) and silymarin (SIL) were prepared and evaluated on breast cancer cell line, MCF-7. METHODS: Nanoparticles (NPs) were formulated by reverse microemulsion method and characterized by DLS, SEM and VSM. The in vitro drug release, cellular cytotoxicity, and MRI properties of NPs were determined as well. The cellular uptake of NPs by MCF-7 cells was investigated through LysoTracker Red staining using confocal microscopy. RESULTS: The MTT results showed that the IC50 of CUR + SIL loaded mSiO2@SPIONs was reduced about 50% in comparison with that of the free drug mixture. The NPs indicated proper MRI features and cellular uptake through endocytosis. CONCLUSION: In conclusion the prepared formulation may offer a novel theranostic system for breast cancer researches.
Subject(s)
Breast Neoplasms , Curcumin , Magnetite Nanoparticles , Nanoparticles , Silymarin , Humans , Female , Curcumin/pharmacology , Silicon Dioxide , Breast Neoplasms/diagnostic imaging , Breast Neoplasms/drug therapy , Silymarin/pharmacologyABSTRACT
The mutual relationship between surface atmospheric pollutants and Cloud-to-Ground (CG) lightning is studied in the Tehran area for the first time. The impact of surface pollutant concentration of PM10 on CG lightning initiation, as well as the production of NO2 and surface ozone derived by lightning occurrence is investigated. To proceed, the reports of thunderstorm occurrence, including CG lightning in the Tehran area have been collected for years of study from the Iran meteorological organization (IRIMO). The surface pollution data are obtained from the Air Quality Control Center (AQCC) for several stations in the area of interest. The number of lightning (NoL) associated with the selected dates is obtained using the World Wide Lightning Location Network (WWLLN). The correlation coefficients associated with the CG lightning and the concentrations of PM10, NO2, and surface ozone are calculated. The hourly variations of accumulated NoL and NO2 and surface ozone are also compared for 24 hours before and after the lightning activity for four days of study during the years of 2009-2013. The results show that there is a positive correlation between PM10 concentration and lightning flash number, obtained from observational data of WWLLN. Moreover, the comparison of NoL and surface pollutant concentration indicates a clear positive contribution from CG lightning in NO2 and ozone production. In days with a considerable number of lightning occurrences, the comparison of the hourly average of NO2 and O3 concentrations with the lightning flash number reveals that NO2 decreases, and O3 increases due to the significant increases of lightning strikes.
Subject(s)
Air Pollutants , Air Pollution , Environmental Pollutants , Lightning , Ozone , Air Pollutants/analysis , Air Pollution/analysis , Environmental Monitoring , Iran , Nitrogen Dioxide/analysis , Ozone/analysis , Particulate Matter/analysisABSTRACT
Theranostic polymeric NPs developed for both cancer diagnosis and cancer therapy. This multifunctional polymeric vehicle was prepared by a single emulsion evaporation method, using carboxyl-terminated PLGA. LHRH as a targeting moiety, was conjugated to the surface of polymeric carrier by applying polyethylene glycol. The results indicated that the diameter of NPs was ~185.4±4.6nm as defined by DLS. The entrapment efficacy of docetaxel, silibinin, and SPIONs was 84.6±4.1%, 80.6±2.7%, and 77.9±4.3%, respectively. The NPs showed a triphasic in-vitro drug release pattern. MTT assay was done on two cell lines, MCF-7 and SKOV-3. Enhanced cellular uptake ability of the targeted NPs to MCF-7 was evaluated in-vitro by confocal laser scanning microscopy. The results indicated that compared to non-targeted NPs, the LHRH targeted NPs had significant efficacy at IC50 concentration. The effect of the NPs on VEGF expression in MCF-7 and SKOV-3 cells was investigated by Real-Time PCR method. VEGF mRNA level expression in MCF-7 cell line reduced by 83% in comparison to control cell line. The designed NPs can be used as promising multifunctional platform for detection and targeted drug delivery in breast cancer.
