Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Stem Cells ; 29(11): 1752-62, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21948558

ABSTRACT

Mouse and human somatic cells can either be reprogrammed to a pluripotent state or converted to another lineage with a combination of transcription factors suggesting that lineage commitment is a reversible process. Here we show that only one factor, the active intracellular form of Notch1, is sufficient to convert mature pigmented epidermal-derived melanocytes into functional multipotent neural crest (NC) stem-like cells. These induced NC stem cells (iNCSCs) proliferate as spheres under stem cell media conditions, re-express NC-related genes, and differentiate into multiple NC-derived mesenchymal and neuronal lineages. Moreover, iNCSCs are highly migratory and functional in vivo. These results demonstrate that mature melanocytes can be reprogrammed toward their primitive NC cell precursors through the activation of a single stem cell-related pathway. Reprogramming of melanocytes to iNCSCs may provide an alternate source of NCSCs for neuroregenerative applications.


Subject(s)
Cellular Reprogramming/physiology , Melanocytes/cytology , Melanocytes/metabolism , Neural Crest/cytology , Neural Stem Cells/cytology , Receptor, Notch1/metabolism , Stem Cells/cytology , Animals , Blotting, Western , Cell Differentiation/genetics , Cell Differentiation/physiology , Cell Line , Cell Movement/genetics , Cell Movement/physiology , Cellular Reprogramming/genetics , Chick Embryo , Humans , Neural Stem Cells/metabolism , Receptor, Notch1/genetics , Stem Cells/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...