Bioaccumulation of trace metals by the gastropod Phorcus lineatus (Da Costa, 1778) from the Atlantic coast of El Jadida, Morocco.

Heavy metals in coastal ecosystems represent an issue for human and environmental health worldwide. The objective of this study was to assess the state of health of Morocco's Atlantic coastline in the El Jadida region. The study was based on metal bioaccumulation in Phorcus lineatus, a species common to Atlantic coasts. Two polluted sites were selected: Haouzia (H) and Jorf-Lasfar (J). Animals were sampled monthly for determination of trace metals in the soft tissues of P. lineatus. Except for Cr, the results show a significant variation in bioaccumulation. The highest concentrations (µg g-1 dry weight) were found in individuals from J, with 10.68 ± 2.43 Cd, 107.36 ± 21.15 Zn, 666.55 ± 143.34 Fe and 184.47 ± 49.80 Cu. The maximum Metal Pollution Index value was 37.06 at site H in spring and 65.96 at site J in autumn. The J site appears significantly more polluted than H site.

Bioaccumulation of trace metals in edible terrestrial snails, Theba pisana and Otala spp., in a dumpsite area in Morocco and assessment of human health risks for consumers.

This study assessed the bioaccumulation patterns of five trace metals (Cd, Cr, Co, Cu, and Zn) in two edible snail species, Theba pisana and Otala spp., collected from a dumpsite in Safi City, Morocco. The results indicated that bioaccumulation might be species-specific, as metal concentration profiles varied between the two snail species. Additionally, higher metal levels in the dumpsite snails confirmed their potential as bioindicators of trace metal pollution in terrestrial environments. However, the distribution of trace elements within the edible parts of the snails showed marked unevenness, with the viscera accumulating more metals than the foot. The study also evaluated the potential human health risks associated with consuming these snails. Trace metal levels in the edible parts exceeded most international safety thresholds. The estimated daily intakes (EDIs) of trace metals through snail consumption were below the provisional tolerable daily intakes (PTDIs) for both children and adults, suggesting that daily consumption is generally safe. Nonetheless, the hazard index (HI) indicated that children might face health risks from long-term consumption of contaminated snails (HI > 1), while adults are less likely to experience such complications (HI < 1). The total target carcinogenic risk (TTCR) was below 1E-04 for both children and adults, indicating negligible to acceptable carcinogenic risks for all consumer groups.

The goose barnacle Pollicipes pollicipes as a tool for trace metal biomonitoring and health risk assessment for human consumers in northwestern Atlantic coast of Morocco.

The bioaccumulation of trace metals Cd, Cr, Cu, Fe, and Zn in soft tissues of the barnacle Pollicipes pollicipes was investigated seasonally along the Atlantic coast of northwestern Morocco. Average concentrations (µg g-1 dry weight) exhibited a decreasing order: Fe (548.15 ± 132.43) > Zn (430.80 ± 181.68) > Cd (17.46 ± 9.99) > Cu (7.72 ± 1.26) > Cr (3.12 ± 0.80), with the highest levels during wet seasons. The "Metal Pollution Index" and "Individual Multimetal Bioaccumulation Index" revealed a substantial barnacle contamination in industrialized areas. Additionally, Cd and Zn concentrations surpassed permissible guideline limits. While the "Target Hazard Quotient" and "Hazard Index" unveiled no significant health risks associated with barnacle consumption for humans, Cd posed potential risks, particularly for children consuming barnacles from polluted locations. Regarding the "Maximum Safe Consumption", Cd demonstrated potential harm across all sex and age groups. These findings contribute valuable data on the safety of barnacle consumption, marking the initial assessment of such risks in Morocco. The study offers evidence of metal pollution occurrence and proposes the barnacle species as a reliable biomonitor of trace metal bioavailabilities in marine coastal areas. To our knowledge, this investigation is the first comprehensive report of metal contamination biomonitoring using barnacles from Moroccan Atlantic waters.

Soil contamination by trace metals and assessment of the risks associated: the dumping site of Safi city (Northwest Morocco).

The main objective of this work was to determine the soil contamination with trace metals within and around the dumpsite of Safi city (Morocco) and to evaluate the potential environmental risk associated. The results showed that the average soil concentrations of trace metals had the following order: Fe > Zn > Cu > Cr > Cd and exceeded the world and the upper continental background concentrations except for Fe. In addition, the concentrations of Zn, Cu, and Cd remained beyond the limit standards given by the WHO/FAO. Geoaccumulation index, enrichment factor, and pollution load index (PLI) indicated that the dumpsite soil is highly contaminated and deteriorated, presenting evidence of high ecological risk proved by the values of the potential ecological risk index (PERI). Correlation analyses revealed a strong relationship between the organic matter & [Fe, Zn, Cr, Cd], calcium carbonates & [Zn, Cr], and Cr & Cu inside the dumpsite soil. Principal component analysis confirmed the temporal and spatial classification of Zone A as the oldest and Zone C as the youngest and indicated that the regrouped trace metals could have the same behavior and or the same origin. The interpolation of trace metals concentrations and PERI revealed a plausible extension outside the landfill, confirmed by PLI values.

Diversity of Medicinal Plants Used by the Local Communities of the Coastal Plateau of Safi Province (Morocco).

Traditional herbal medicine is still used for basic healthcare by a significant portion of the population in developing countries. This study aimed to explore the medicinal plant's diversity and to document related traditional knowledge in the Safi region of Morocco. We used semi-structured questionnaires to interview 222 informants living in the study area. To perform data analysis, we used quantitative indices like use value (UV), family use value (FUV), fidelity level (FL), the relative popularity level (RPL), rank of order priority (ROP), and informant consensus factor (ICF). We reported the ethnomedicinal uses of 144 medicinal plants belonging to 64 families. According to the findings, the dominating families were Lamiaceae (17 taxa), Asteraceae (15 taxa), and Apiaceae (12 taxa). The most commonly utilized plant part (48%) was leaves. The decoction was reported as the main preparation method (42%). Highly cited plant species were Marrubium vulgare (UV = 0.56), Salvia rosmarinus Spenn. (UV = 0.47), Thymus serpyllum (UV = 0.32), and Dysphania ambrosioides (UV = 0.29). Papaveraceae (FUV = 0.26), and Urticaceae (FUV= 0.23), Geraniaceae (FUV = 0.17), Oleaceae (FUV = 0.17), Lamiaceae (FUV = 0.17) had the highest family use-values. Gastrointestinal disorders (88%), respiratory diseases (85%), and anemia (66%) have the greatest ICF values. This study reveals the indigenous people's reliance on plant-derived traditional medicine to prevent, alleviate, and treat a broad range of health concerns. Our findings will provide a scientific basis for ethnomedicinal legacy conservation and further scientific investigations aimed at new natural bioactive molecules discovery.

Trace metal contents in the mussel Mytilus galloprovincialis from Atlantic coastal areas in northwestern Morocco: Levels of contamination and assessment of potential risks to human health.

The bioaccumulation of trace metals Cd, Cr, Cu and Zn in soft tissues of the mussel Mytilus galloprovincialis was studied seasonally along the Atlantic coast of northwestern Morocco. Average concentrations (µg g-1 dry weight) decreased in the following order: Zn (141.61 ± 78.06) > Cr (22.57 ± 15.47) > Cu (18.95 ± 20.01) > Cd (16.33 ± 19.01), with higher amounts during wet seasons. The "Metal Pollution Index" and "Individual Multimetal Bioaccumulation Index" suggested a higher mussel contamination in industrialized areas. Moreover, Cd and Cr concentrations were above the permissible guidelines' limits. The related risks to human health associated with mussel consumption were also assessed. The "Target Hazard Quotient" for Cd and Cr revealed a threat to children when mussels originated from polluted stations, while the "Hazard Index" indicated a hazard to them in all stations. Regarding the "Maximum Safe Consumption", children should not consume mussels, even in low quantities. This study represents the first datum in Morocco to assess such a risk to human health.

Origin and distribution of desert ants across the Gibraltar Straits.

The creation of geographic barriers has long been suspected to contribute to the formation of new species. We investigated the phylogeography of desert ants in the western Mediterranean basin in order to elucidate their mode of diversification. These insects which have a low dispersal capacity are recently becoming important model systems in evolutionary studies. We conducted an extensive sampling of species belonging to the Cataglyphis albicans group in the Iberian Peninsula (IP) and the northern Morocco (North Africa; NA). We then combined genetic, chemical and morphological analyses. The results suggest the existence of at least three and five clades in the IP and NA, respectively, whose delineation partially encompass current taxonomic classification. The three Iberian clades are monophyletic, but their origin in NA is uncertain (79% and 22% for Bayesian and Maximum Likelihood support, respectively). The estimation of divergence time suggests that a speciation process was initiated after the last reopening of the Gibraltar Straits ≈5.33 Ma. In the IP, the clades are parapatric and their formation may have been triggered by the fragmentation of a large population during the Pleistocene due to extended periods of glaciation. This scenario is supported by demographic analyses pointing at a recent expansion of Iberian populations that contrasts with the progressive contraction of the NA clades. Niche modeling reveals that this area, governed by favorable climatic conditions for desert ants, has recently increased in the IP and decreased in NA. Altogether, our data points at geoclimatic events as major determinants of species formation in desert ants, reinforcing the role of allopatric speciation.

Seasonal dynamics of ant community structure in the Moroccan Argan Forest.

In this study we describe the structure and composition of ant communities in the endemic Moroccan Argan forest, using pitfall traps sampling technique throughout the four seasons between May 2006 and February 2007. The study focused on two distinct climatic habitats within the Essaouira Argan forest, a semi-continental site at Lahssinate, and a coastal site at Boutazarte. Thirteen different ant species were identified, belonging to seven genera. Monomorium subopacum Smith and Tapinoma simrothi Krausse-Heldrungen (Hymenoptera: Formicidae) were the most abundant and behaviorally dominant ant species in the arganeraie. In addition, more specimens were captured in the semi-continental site than in the coastal area. However, no significant difference was observed in species richness, evenness, or diversity between both sites. Composition and community structure showed clear seasonal dynamics. The number of species, their abundance, their diversity, and their evenness per Argan tree were significantly dissimilar among seasons. The richness (except between summer and autumn), and the abundance and the evenness of ant species among communities, showed a significant difference between the dry period (summer and spring) and the rainy period (winter and autumn). Higher abundance and richness values occurred in the dry period of the year. Ant species dominance and seasonal climatic variations in the arganeraie might be among the main factors affecting the composition, structure, and foraging activity of ant communities. This study, together with recent findings on ant predation behavior below Argan trees, highlights the promising use of dominant ant species as potential agents of Mediterranean fruit fly bio-control in the Argan forest and surrounding ecosystems.