Challenges and technological interventions in rice-wheat system for resilient food-water-energy-environment nexus in North-western Indo-Gangetic Plains: A review.

Rice (Oryza sativa L.)-wheat (Triticum aestivum L.) cropping system in north-western Indo-Gangetic Plains performed a crucial role in the national food security. However, the widespread and intensive cultivation of this system has led to serious problems such as declining groundwater table (~1 meter year-1) with sharp increase in number of districts under over-exploitation category, residue burning, higher greenhouse gases emission and herbicide resistance in weeds, causing stagnant crop productivity and lesser profitability. In this review article, an attempt has been made to discuss the major issues pertaining to intensive rice-wheat cultivation amidst climate vagaries and futuristic approach to address these challenges. Different tillage- and crop-specific recommendations such as adoption of direct seeded rice, diversification with lesser resource guzzling crops such as maize (Zea mays L.) at least on the periodic manner especially in light-medium soils, inclusion of summer legumes and alternative tillage systems (permanent beds and zero tillage with residue retention) have been suggested to address these issues. However, crop performance under these techniques has been found to be location, soil and cultivar specific. The absence of aerobic tailored genotypes and weeds have been identified as the major constraints in adoption of direct seeded rice. The integrated strategies of conservation tillage, crop breeding program and resource conserving region- and soil-specific agronomic measures with crop diversification would be helpful in tackling the sustainability issues. It requires future efforts on developing crop genotypes suited to conservation tillage, effective weed control strategies and trainings and demonstrations to farmers to switch from conventional rice-wheat system to alternative cropping systems.

Biochemical characterization and enhanced production of endoxylanase from thermophilic mould Myceliophthora thermophila.

Endoxylanase production from M. thermophila BJTLRMDU3 using rice straw was enhanced to 2.53-fold after optimization in solid state fermentation (SSF). Endoxylanase was purified to homogeneity employing ammonium sulfate precipitation followed by gel filtration chromatography and had a molecular mass of ~ 25 kDa estimated by SDS-PAGE. Optimal endoxylanase activity was recorded at pH 5.0 and 60 °C. Purified enzyme showed complete tolerance to n-hexane, but activity was slightly inhibited by other organic solvents. Among surfactants, Tweens (20, 60, and 80) and Triton X 100 slightly enhanced the enzyme activity. The Vmax and Km values for purified endoxylanase were 6.29 µmol/min/mg protein and 5.4 mg/ml, respectively. Endoxylanase released 79.08 and 42.95% higher reducing sugars and soluble proteins, respectively, which control after 48 h at 60 °C from poultry feed. Synergistic effect of endoxylanase (100 U/g) and phytase (15 U/g) on poultry feed released higher amount of reducing sugars (58.58 mg/feed), soluble proteins (42.48 mg/g feed), and inorganic phosphate (28.34 mg/feed) in contrast to control having 23.55, 16.98, and 10.46 mg/feed of reducing sugars, soluble proteins, and inorganic phosphate, respectively, at 60 °C supplemented with endoxylanase only.

Enhanced endoxylanase production by Myceliophthora thermophila with applicability in saccharification of agricultural substrates.

The production of enzymes by solid-state fermentation is an interesting process and currently used worldwide as it can be carried out in solid matrix in absence of free water. In present study, Myceliophthora thermophila BJTLRMDU3 produced high titres of endoxylanase (890.55 U/g DR, dry residue) using 5 g rice straw at pH 7.0 and at 45 °C with 1:7 (w/v) solid-to-moisture ratio with inoculum rate of 12 × 106 spores/ml after 4 days in solid-state fermentation. High enzyme titre was produced after moistening the rice straw with solution containing ammonium sulphate (0.4%), K2HPO4 (1.0%), MgSO4·7H2O (0.3%), FeSO4·7H2O (0.03%) and CaCl2 (0.03%). Addition of sucrose (2% w/v) and ammonium nitrate (2% w/v) further enhanced the endoxylanase production. A high endoxylanase production was achieved at water activity (a W) of 0.95 (1639.80 U/g DR) that declined drastically below this value. Among different surfactants, Tween 20 (3% v/v) enhanced the secretion of endoxylanase (2047.91 U/g DR). Furthermore, on optimization of K2HPO4 concentration, it was found that 0.5% K2HPO4 improved (2191.28 U/g DR) endoxylanase production and overall 4.35-folds increase in production of endoxylanase was achieved after optimization of culture conditions. The enzyme has potential to liberate monomeric (xylose) as well as oligomeric (xylotiose, xylotetrose, and xylopantose) sugars from xylan. On saccharification of rice straw and corncob with endoxylanase, maximum yield of reducing sugars was 135.61 and 132.61 mg/g of substrate recorded after 48, and 36 h, respectively.