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Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth.

Dahl, Anja Lindis; Friis, Martin Barfred; Hallberg, Hanne Wiese; Kristiansen, Gitte Qvist; Holzknecht, Barbara Juliane.

Diagn Microbiol Infect Dis ; 109(2): 116233, 2024 Jun.

Article in English | MEDLINE | ID: mdl-38547798

ABSTRACT

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Subject(s)

Bacterial Proteins , Gram-Positive Bacterial Infections , Polymerase Chain Reaction , Sensitivity and Specificity , Vancomycin-Resistant Enterococci , Humans , Vancomycin-Resistant Enterococci/genetics , Vancomycin-Resistant Enterococci/isolation & purification , Vancomycin-Resistant Enterococci/drug effects , Polymerase Chain Reaction/methods , Bacterial Proteins/genetics , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Bacterial Infections/diagnosis , Rectum/microbiology

ABSTRACT

Subject(s)

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