ABSTRACT
OBJECTIVE: To evaluate the correlation of collateral circulation with prognosis in patients with acute cerebral infarction. METHODS: A total of 260 patients with acute ischemic stroke within 1 week of symptom onset underwent digital subtraction angiogram (DSA). The National Institutes of Health Stroke Scale (NIHSS) scores were obtained at admission. And the Modified Rankin scores (mRS) were assessed at a 3-month follow-up. The follow-up data were acquired through clinic visits or telephone interviews. RESULTS: Among them, 86 were found to have intra- or extra-cranial culprit artery severe stenosis or occlusion. And 36 (75.00%) in 48 patients had collateral arterial circulation while 11 (28.64%) in 38 patients posterior circulation. There were statistical differences in the NIHSS scores at admission and favorite clinical outcome (mRS ≤ 2) at 3-month follow-up for patients with and without collateral circulation. CONCLUSION: DSA is the golden standard for the assessment of collateral circulation in patients with severe cerebral artery stenosis or occlusion. The prognosis is better in stroke patients with collateral circulation.
Subject(s)
Cerebral Infarction/physiopathology , Collateral Circulation , Aged , Angiography, Digital Subtraction , Carotid Stenosis/complications , Cerebral Infarction/complications , Cerebral Infarction/diagnostic imaging , Cerebrovascular Disorders/complications , Female , Humans , Male , Middle Aged , Prognosis , Treatment OutcomeABSTRACT
Activated mTOR was implicated to play a role in the carcinogenesis of nasopharyngeal carcinoma (NPC). However, the mechanism of activated mTOR/Complex1(mTORC1) signaling pathway in NPC development has not been well established. In this study, we correlated the expression of mTORC1 signal molecules and Cyclin D1 in NPC. We also investigated the effect of blocking mTORC1 signal with rapamycin and mTOR siRNA on Cyclin D1 expression in CNE-2 cells, as well as cell apoptosis and viability. We found a positive association of mTORC1 signal molecules and Cyclin D1 in NPC. Also, we found blockage mTORC1 inhibited Cyclin D1 expression in CNE-2 cells and enhanced cell apoptosis. Our results suggested that mTORC1 signal pathway might be a potential target for NPC therapy.
Subject(s)
Carcinoma/metabolism , Cyclin D1/metabolism , Nasopharyngeal Neoplasms/metabolism , Protein Kinases/metabolism , Signal Transduction , Adaptor Proteins, Signal Transducing/metabolism , Adult , Apoptosis , Carcinoma/pathology , Carcinoma/therapy , Case-Control Studies , Cell Cycle Proteins , Cell Line, Tumor , Cell Survival , Dose-Response Relationship, Drug , Female , Humans , Male , Middle Aged , Multiprotein Complexes/metabolism , Nasopharyngeal Neoplasms/pathology , Nasopharyngeal Neoplasms/therapy , Phosphoproteins/metabolism , Phosphorylation , Protein Kinases/genetics , RNA Interference , RNA, Small Interfering/metabolism , Signal Transduction/drug effects , Sirolimus/pharmacology , TOR Serine-Threonine Kinases , Time Factors , Transfection , Up-RegulationSubject(s)
Carotid Stenosis/therapy , Coronary Disease/therapy , Stents , Aged , Female , Follow-Up Studies , Humans , Male , Middle Aged , Stents/adverse effectsABSTRACT
OBJECTIVE: To preliminarily evaluate the immunity and safety of the recombinant adenoviruses expressing rotavirus structural proteins VP7 and VP6 in rhesus monkeys to lay a foundation for the development of novel genetic engineering vaccine against rotavirus. METHODS: Baby monkeys were immunized with the recombinant adenoviruses intranasally or orally. Serum IgG against rotavirus was measured with ELISA. During the course of the immunization, besides the daily monitoring of body temperature, weight and clinical symptoms, the routine blood and urine tests and liver and kidney function tests were also conducted. RESULTS: Monkeys immunized via intranasal or oral routes could both generate serum IgG against rotavirus. During the immunization, the temperature of monkeys was normal and body weight raise stably. Both routine blood and urine tests and liver and kidney function tests showed no significant alteration compared with the control group. CONCLUSION: The immunization with the recombinant adenoviruses expressing rotavirus antigens is able to induce rotavirus specific efficient immune responses and is safe to baby rhesus monkeys. The preliminary results implied that the recombinant adenoviruses could be an ideal vaccine for rotavirus and lay a foundation for further studies.
