[Epitope screening of influenza A (H3N2) by using phage display library].

OBJECTIVE: To screen the influenza A (H3N2) mimotopes by using phage display library. METHODS: Using influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. RESULTS: 21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2). CONCLUSION: Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.