ABSTRACT
Two new dinuclear Ru(II) polypyridyl complexes containing three and ten methylene chains in their bridging linkers are synthesized and characterized. Their calf thymus DNA-binding and plasmid DNA photocleavage behaviors are comparatively studied with a previously reported, six-methylene-containing analog by absorption and luminescence spectroscopy, steady-state emission quenching by [Fe(CN)6](4-), DNA competitive binding with ethidium bromide, DNA viscosity measurements, DNA thermal denaturation, and agarose gel electrophoresis analyses. Theoretical calculations applying the density functional theory (DFT) method for the three complexes are also performed to understand experimentally observed DNA binding properties. The results show that the two complexes partially intercalate between the base pairs of DNA. Cellular uptake and colocalization studies have demonstrated that the complexes could enter HeLa cells efficiently and localize within lysosomes. The in-vitro antitumor activity against HeLa and MCF-7 tumor cells of the complexes are studied by MTT cytotoxic analysis. A new method, high-content analysis (HCA), is also used to assess cytotoxicity, apoptosis and cell cycle arrest of the three complexes. The results show that the lengths of the alkyl linkers could effectively tune their biological properties and that HCA is suitable for rapidly identifying cytotoxicity and can be substituted for MTT assays to evaluate the cell cytotoxicity of chemotherapeutic agents.
Subject(s)
DNA/metabolism , Ruthenium Compounds/metabolism , Drug Screening Assays, Antitumor , Electrophoresis, Agar Gel , HeLa Cells , Humans , In Vitro Techniques , MCF-7 Cells , Photochemical Processes , Ruthenium Compounds/pharmacologyABSTRACT
The multilayer films consisting of graphene oxide and a cobalt complex were fabricated though electrostatic layer-by-layer self-assembly technique and characterized by UV-Vis absorption spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and cyclic voltammetry. The results demonstrated that the hybrid films were successfully prepared and exhibited good electrochemical activity. The film was also subjected to photoelectrochemical studies and were found to exhibit large cathodic photocurrent density of 6.1 µA/cm(2) while irradiated with 100 mW/cm(2) polychromatic light (730 nm>λ>325 nm) at an applied potential of -0.4V versus saturated calomel electrode.
ABSTRACT
In the title compound, [Zn(C(10)H(8)N(2))(C(12)H(8)N(2))(H(2)O)(2)](NO(3))(2)·0.5C(10)H(8)N(2)·H(2)O, the Zn(II) atom is coordinated in a distorted octa-hedral geometry by two N atoms from two 4,4'-bipyridine (4,4'-bipy) ligands, two N atoms from a chelating 1,10-phenanthroline ligand and two O atoms from two mutually cis water mol-ecules. The 4,4'-bipy ligands bridge the Zn(II) atoms into a chain structure along [100]. The uncoordinated 4,4'-bipy mol-ecule lies on an inversion center. O-Hâ¯O and O-Hâ¯N hydrogen bonds connect the cationic chains, the nitrate anions, the uncoordinated 4,4'-bipy mol-ecules and the water mol-ecules into tow-dimensional networks.
ABSTRACT
In the title copper(II) salt, [Cu(C(6)H(6)N(4))(2)(C(2)H(6)OS)(2)](BF(4))(2), the Jahn-Teller distorted octa-hedral coordination sphere of copper is formed from four 2,2'-bi-1H-imidazole N atoms and two dimethyl sulfoxide O atoms. The Cu atom lies on a center of inversion. N-Hâ¯O and N-Hâ¯F hydrogen bonds give rise to a one-dimensional structure. The BF(4) (-) anion is disordered over two sites in a 0.671â (10):0.329â (10) ratio.
