[Effect of insulin on lipid peroxidation in the erythrocyte membranes of patients with insulin dependent diabetes mellitus]. / Vliianie insulina na razvitie perekisnogo okisleniia lipidov v membranakh eritrotsitov pri insulinzavisimom sacharnom diabete.

The spin-probe techniques has been used to study insulin-membranes interaction in erythrocytes by insulin dependent diabetes mellitus. The lipid peroxidation has been initiated by FeSO4-ascorbate. It was found the insulin defend the lipids in control by peroxidation, but no effect in insulin dependent diabetes mellitus erythrocytes. The mechanisms of the insulin-influence on the lipids peroxidation are discussed.

[The structural status of red blood cell membranes in children with insulin-dependent diabetes mellitus. Examination by spin probe]. / Strukturnoe sostoianie membran eritrotsitov krovi detei, bol'nykh insulinozavisimym sakharnym diabetom. Issledovanie metodom spinovogo zonda.

Using spin probes-stearic acid analogues, the authors investigated the microviscosity and the a/b parameter of red blood cell membranes in children with diabetes mellitus. The structural changes were correlated with altered metabolic measurements. The change in the pathway of insulin in diabetes mellitus led to the structural state of red blood cell membranes.

[ESR-spectroscopy in medical practice and biomedical research]. / EPR-spektroskopiia v prakticheskoi meditsine i mediko-biologicheskikh issledovaniiakh.

The authors describe the use of the ESR technique during the past 20 years. There are many examples of the application of the technique in different investigations. The authors propose the new way of training medical students to apply it.

[Recording of surface potential changes in erythrocyte membranes from patients with insulin-dependent diabetes mellitus using a spin probe method]. / Registratsiia metodom spinovogo zonda izmenenii poverkhnostnogo potentsiala membran éritrotsitov krovi bol'nykh insulinzavisimym sakharnym diabetom.

Changes in surface potential of erythrocyte membranes were studied by a positively charged analog as a spin probe. Insulin-dependent diabetes mellitus (IDDM) patients demonstrated a significant increase (by 16 +/- 2.0 mV) in negative surface potential as compared to the control. An important role of this phenomenon in pathogenesis of IDDM is discussed.

[Structural changes in erythrocyte membranes in diabetes mellitus using spin labelled fatty acids]. / Issledovanie s pomoshch'iu spin-mechenykh zhirnykh kislot strukturnykh izmenenii v membranakh éritrotsitov pri sakharnom diabete.

Microviscosity and polarity of erythrocyte membranes of the blood of patients suffering from diabetes were studied by ESR using spin-labeled fatty acids. Structural changes were discovered 0.6-0.8 nm from the membrane surface in the lipid bilayer of erythrocytes obtained from patients' blood. No essential immobilization of the acyl chains of phospholipids was found in deeper layers as compared with the control. The bilayer polarity at different depth was not essentially changed either.

[Using the spin label and probe method in medical research]. / Ispol'zovanie metoda spinovykh metok i zondov v meditsinskikh issledovaniiakh.

Spin probe methods applied in membrane medical research are reviewed. The results of erythrocyte membrane studies in diabetes demonstrate potentialities of the methods in medical investigations. The new data on the changes occurring in the erythrocyte membranes of patients suffering from type I diabetes are under discussion.

[Changes in the membrane structure of the brain microsomes in cats with electrodes implanted in the inner ear]. / Izmeneniia v strukture membran mikrosom mozga koshek pri implantatsii élektrodov vo vnutrennee ukho.

Using spin-labels it was shown that electrode implantation into the cats scala media resulted in structure reconstruction of microsome membranes of auditory zones in cats brain. Membrane fluidity increased but there was no change of surface potential. The changes of microviscosity were found during the first month after the implantation. Five months later the order parameter returned to the mean valve for cats in control group without electrodes.

[The auditory area of the cat cerebral cortex after implantation of electrodes into the inner ear (morphologic and biophysical research)]. / Slukhovaia zona kory golovnogo mozga koshki posle implantatsii élektrodov vo vnutrennee ukho (morfologicheskoe i biofizicheskoe issledovanie).

The article presents the findings of experimental studies of morphological and biophysical changes in the A1 hearing zone of the cerebral cortex depending on the period of the electrodes implantation in the inner ear. The studies showed that in the process of long implantation in the neurones of the hearing zone one can notice the disorders of water-electrolytic exchanges. There is a definite correlation between structural changes in microsome membranes of A1 zone and morphological data.

[Use of the spin label method for studying the structure of the brain membranes in cats with streptomycin poisoning]. / Primenenie metoda spinovykh zondov dlia izucheniia struktury membran mozga koshek pri streptomitsinovoi intoksikatsii.

The structural changes in cat brain membranes under the injections of intramuscular streptomycin which is ototoxic antibiotic have been studied. The increase of membrane microviscosity in brain areas which are the direct projection of the auditory way has been revealed using fat acidic spin probe on the basis of stearic acid. The changes in membranes of other brain areas have not been found that exhibits a specific streptomycin influence on the auditory analyzer. The EPR spectra of the hydrocarbon spin label C12H25 localizing in near membrane region don't change in brain membranes of experimental animals compared with the normal ones.