Morphometric approaches to Cannabis evolution and differentiation from archaeological sites: interpreting the archaeobotanical evidence from bronze age Haimenkou, Yunnan.

Cannabis grains are frequently reported from archaeological sites in Asia, and hypothesized centers of origins are China and Central Asia. Chinese early cannabis remains are often interpreted as evidence of hemp fabric production, in line with early textual evidence describing ritualistic hemp cloth use and hemp cultivation as a grain crop. Modern measurements on cannabis varieties show distinct sizes between fibre or oil/fibre and psychoactive varieties, the former having larger seeds on average than the latter. This paper reviews the current macro-botanical evidence for cannabis across East, Central and South Asia and builds a comparative framework based on modern cannabis seed measurements to help identify cannabis use in the past, through the metric analysis of archaeologically preserved seeds. Over 800 grains of cannabis were retrieved from the 2008 excavation of Haimenkou, Yunnan, Southwest China, dating to between 1650 and 400 bc. These are compared with other known archaeological cannabis and interpreted through the metric framework. This offers a basis for exploration of the seed morphometrics potential to infer cannabis cultivation and diversification in uses. At Haimenkou, cannabis seeds size mostly plot in the range of overlapping psychoactive/fibre types; we therefore suggest that the cannabis assemblage from Haimenkou is indicative of a crop beginning to undergo evolution from its early domesticated form towards a diversified crop specialized for alternative uses, including larger oilseed/fibre adapted varieties. Supplementary Information: The online version contains supplementary material available at 10.1007/s00334-023-00966-6.

Archaeological and molecular evidence for ancient chickens in Central Asia.

The origins and dispersal of the chicken across the ancient world remains one of the most enigmatic questions regarding Eurasian domesticated animals. The lack of agreement concerning timing and centers of origin is due to issues with morphological identifications, a lack of direct dating, and poor preservation of thin, brittle bird bones. Here we show that chickens were widely raised across southern Central Asia from the fourth century BC through medieval periods, likely dispersing along the ancient Silk Road. We present archaeological and molecular evidence for the raising of chickens for egg production, based on material from 12 different archaeological sites spanning a millennium and a half. These eggshells were recovered in high abundance at all of these sites, suggesting that chickens may have been an important part of the overall diet and that chickens may have lost seasonal egg-laying.

Agriculture along the upper part of the Middle Zarafshan River during the first millennium AD: A multi-site archaeobotanical analysis.

The Zarafshan River runs from the mountains of Tajikistan and terminates in the sands of the Kyzyl-Kum Desert in Uzbekistan; it served as a communication route and homeland for the Sogdians. The Sogdians are historically depicted as merchants existing from the end of the first millennium BC through the first millennium AD. While recent research has provided the first glimpse into cultivation, commerce, communication, and consumption in the Lower Zarafshan, the agricultural heartland of the Middle Zarafshan Basin has remained unstudied. This paper presents the results of archaeobotanical investigations conducted at five ancient urban sites/areas spanning the fifth to the twelfth centuries AD: Kainar (Penjikent citadel), Penjikent (shahristan), Sanjar-Shah, Kuk-Tosh (pre-Mongol Penjikent), and Afrasiab. Collectively, these data show that cereals, legumes, oil/fiber crops, fruits, and nuts were cultivated on the fertile Zarafshan floodplains. In this paper, we discuss evidence for the diversification of the agricultural assemblage over time, including the introduction of new staple crops and fruits into an already complex cultivation system. In addition, we contrast our data with previously published results from sites along the course of the Zarafshan to determine whether there is a dietary difference between pre-and post-Islamic conquest periods at settlements located along the river.

Food production and agricultural systems on the southwestern frontier of the Han Empire: archaeobotanical remains from the 2016 excavation of Hebosuo, Yunnan.

Dian Basin in Yunnan province is an important center for both early agricultural production and centralized state formation. Settled agricultural villages are present in the province since at least the third millennium BC, and by the first millennium BC, the Dian Culture, a highly specialized bronze polity, flourished in the Dian Basin and surrounding area, until it was conquered by the Han in 109 BC. The increased deployment of flotation at recent archaeological excavations in Yunnan allowed the reconstruction of agricultural practices from the Neolithic to the early Bronze Age, documented at Baiyangcun, Haimenkou, and Xueshan among others. However, archaeobotanical evidence relating to the pivotal period right before and after the Han conquest have so far been lacking, with only limited written records about agricultural production in the Shiji by Sima Qian. Here we present for the first time direct archaeobotanical evidence relating to this transitional period as revealed by rich Han period deposits found during the 2016 excavation of Hebosuo, the largest Dian settlement investigated in Yunnan so far, dated by direct AMS on charred cereal grains and artefactual evidence as spanning from between 850 BC-220 AD. Following the Han conquest, the main components of the agricultural system did not undergo radical changes, but the weedy flora indicates a heavier reliance of wet-land rice systems, evidencing a higher level of water management or even irrigation practices, and the consequent intensification of the agricultural production. These findings on shifting agricultural regimes in Yunnan also contribute to current debates about the interplay between intensification, food risk, and ecology in times of political instability. Supplementary Information: The online version contains supplementary material available at 10.1007/s12520-023-01766-9.

The origins of multi-cropping agriculture in Southwestern China: Archaeobotanical insights from third to first millennium B.C. Yunnan.

Yunnan's location at the crossroad of temperate China, Northeast India and tropical mainland Southeast Asia makes it a pivotal area for the understanding of early cultural contacts and agricultural spread between these ecologically diverse regions. This paper evaluates current evidence relating to the emergence of the first agricultural systems in Yunnan. It also reviews previous theories on agricultural dispersal to Yunnan, including whether Austroasiatic speakers were responsible for the spread of rice from Yunnan to mainland Southeast Asia, and builds a new framework that allows to tie agricultural development in the region into broader patterns of early migration and exchange networks. Archaeobotanical remains attest to an initial spread of rice and millet from Central China into Yunnan in the third millennium B.C. and the establishment of a mixed-crop economy; the introduction of wheat and barley in the second millennium B.C. allowed for increased diversification of the agricultural system, with a two-season intensification trend in the late first millennium B.C. Differences in early rice cultivation ecologies between Yunnan and mainland Southeast Asia suggest that Yunnan rice farmers may not have had a primary role in the southern dispersal of rice, however, more data is needed to fully clarify the source and development of dryland cultivation of rice in mainland Southeast Asia. Supplementary Information: The online version contains supplementary material available at 10.1007/s41826-022-00052-2.