A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis.

A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200-450 ng/µl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.

Preparation of novel mesoporous carbons for the adsorption of an inflammatory cytokine (IL-1 beta).

Mesoporous carbons derived from two types of sulphonated styrene divinylbenzene copolymers (Macronet MN500HS and CT275, Purolite International Ltd) were produced and their adsorptive capacity for the proinflammatory cytokine IL-1 beta (MW 14.4 kDa) determined. The carbons produced had surface areas from 400 to 1200 m(2)g(-1) and pore volume between 0.2 and 1.4 cm(3)g(-1). The mechanical strength of the carbon beads with surface area values up to 800 m(2)g(-1) were robust. The highest adsorption value of IL-1 beta was 150 ng g(-1) for a mesoporous carbon with surface area around 900 m(2)g(-1) and pore volume around 1.3 cm(3)g(-1). However, there was a trade-off between adsorptive capacity and mechanical strength. When used in conjunction with existing treatment modalities, the materials produced have the potential to enhance the removal of uraemic toxins.

Interactive effects of the affect quality and directional focus of mental imagery on pain analgesia.

College students (25 men and 25 women) were randomly assigned (within sex) to each of the 4 factorial groups, based on manipulation of affect quality (positive vs. negative) and directional focus (internal vs. external) of mental imagery, and to a control group receiving no manipulation. Both imagery variables had a significant impact on pain tolerance and ratings during a cold-pressor test with positive affect and external imagery producing greater analgesia than their counterpart conditions. Positive affect imagery combined with external imagery resulted in the lowest reported pain amongst the groups. However, self-reported mood descriptors did not consistently parallel the pain tolerance and rating data. Likewise, although heart rate and skin potential responses increased during the cold pressor for the group as a whole, the only significant difference amongst the experimental groups was the relatively higher skin potential reactivity of the positive affect-external imagery group--possibly reflecting greater task engagement for this group. Seemingly, imagery in this situation operates primarily via cognitive, rather than via physiological mediators of the pain experience.

Misrepresentation of research criteria by orthopaedic residency applicants.

BACKGROUND: Previous studies have shown that applicants for postgraduate training may misrepresent research citations. We evaluated the research citations that were identified in a review of the Publications and Work and Research sections from the Electronic Residency Application Service (ERAS) data for all applicants to our orthopaedic residency program for the 1998 to 1999 academic year. METHODS: The citations were searched for on Medline. We initially used the name of the first author, then the name of the applicant, the name of the journal, the volume number, the issue number, and the page numbers. When a journal was not listed in Medline, an interlibrary search was instituted with use of the same format. When no match was made for any category, the citation was defined as misrepresented. Point estimates are reported as percentages. RESULTS: Publications were listed on sixty-four (30.0 percent) of 213 applications. One hundred and thirty-eight publications were cited; there were fifteen citations (10.9 percent) to book chapters, twenty-six (18.8 percent) to journals not listed in Ulrich's International Periodicals Directory, and twenty-one (15.2 percent) to articles listed as in press, in print, or submitted for publication. Seventy-six articles that had been cited as appearing in journals listed in Ulrich's Directory were checked and verified. Fourteen (18 percent) of these seventy-six publications were misrepresented. Misrepresentations included citations of nonexistent articles in actual journals and nonauthorship of existing articles. CONCLUSIONS: We concluded that publications listed on postgraduate applications should be scrutinized carefully. Copies of cited publications should be required by residency programs before applications are considered complete. The importance of professionalism needs to be emphasized in the curricula of medical schools. Residency training programs should develop guidelines regarding misrepresentation.

Recurrent giant cell tumors of the tendon sheath.

Seventy patients underwent surgical excision of a giant cell tumor of the tendon sheath. The patients were monitored for an average of 3 years 4 months. Nineteen of the 70 patients (27%) had a surgically and histologically documented recurrence at an average of 2 years 3 months (range, 3 months to 10 years) following the initial procedure. Eight of 19 patients (42%) with recurrence had a prior recurrence. Statistically significant risk factors for recurrence included presence of adjacent degenerative joint disease, location at the distal interphalangeal joint of the finger or interphalangeal joint of the thumb, and radiographic presence of an osseous pressure erosion. Age, gender, size, and location within the digit (volar vs. dorsal) were not risk factors for recurrence. Awareness of these associations should be reflected in the surgeon's approach and preoperative discussion with the patient.

Psychology of computer use: XLII. Problem solving and humor as a function of computer anxiety.

From a pool of 115 undergraduate women, 20 were placed in a Computer-anxious group and 20 in a Computer-nonanxious group based on their scores on the Computer Anxiety Scale. Half of the subjects were randomly assigned to a group to which the computer presented the problems first and half to a group to whom the computer presented the jokes first. Electromyograms from subjects' zygomatic muscles were monitored as well as palmar skin conductance. Behavioral measures were collected prior to and following the computer interaction. Although the electromyogram for zygomatic tension (smiles) was higher in the jokes condition, indicating that subjects responded to jokes as humorous, Computer-anxious subjects showed increased scores on anxiety and depression and relatively high skin conductance on the initial joke. Subjects with high computer-anxiety had less experience with computers, had lower scores on the Rosenberg Self-esteem test, and, likely not being familiar with computer humor, reacted anxiously.

No respite during sleep: heart-rate hyperreactivity to rapid eye movement sleep in angry men classified as Type A.

9 men scoring as Type A and Angry and 9 men scoring as Type B and Nonangry on the Jenkins Activity Survey and Spielberger's Trait Anger Expression completed a 1-min. mental arithmetic task and slept through 1 period of rapid eye movement. The Angry Type A scorers showed greater heart-rate increases from baseline to that during mental arithmetic and from nonrapid eye movements to periods of REM than the Nonangry Type B scorers. While the former showed equivalent reactivity to stressors while awake and asleep, the latter group showed less heart-rate reactivity during the period of REM than during mental arithmetic. Neither group yielded clear descriptions of their dreams, and no differences between groups were noted for ratings on aggressivity in dreams. It appears that Angry Type A scorers show cardiovascular hyperreactivity even when asleep in the lab.

Paraspinal EMG and autonomic reactivity of patients with back pain and controls to personally relevant stress.

The reactivity of surface paraspinal EMG was contrasted among groups of (1) patients seeking treatment for chronic back pain, (2) nonpatients reporting chronic back pain, and (3) healthy controls. The EMG response to the personally relevant stressor (all stimuli were 1 min.) tasks was greater for the patient group relative to the other two groups. However, the patients' magnitude of response elicited by the control task was nearly equal to that of the personally relevant task, suggesting that the task demand to "describe a recent event" may be the "personally relevant" stressor component rather than the emotional valence attached to the content of that description.

Effects of humorous stimuli and sense of humor on discomfort.

The effects of humor on increasing discomfort thresholds were tested with Transcutaneous End Nerve Stimulation (TENS). Undergraduate students (n = 31) with high or low scores on Martin and Lecourt's Situational Humor Questionnaire were randomly assigned to a humor or nonhumor condition. Discomfort thresholds for TENS were assessed before and during treatment. There was a significant increase in discomfort thresholds in the humorous treatment compared to the nonhumorous condition. Evidence was found for subjects to smile "wryly" (an increase in zygomatic and corrugator tensions) more during humorous stimuli than nonhumorous stimuli when they were waiting to be stimulated with the TENS.

Effect of exposure to humorous stimuli on induced depression.

In testing the hypothesis that exposure to humor may be used to relieve the symptoms of depression, 38 female undergraduates were shown depressive slides of the Velten (1968) mood statements and then assigned to one of three groups. One group heard a humorous audiotape, the second group heard a nonhumorous tape, while a third waiting control heard no tape. Heart rate and zygomatic (smile) and corrugator (frown) muscle tensions were recoreded during slide and tape presentations. The Multiple Affect Adjective Check List, administered before and after slide and tape presentations, showed depression induction was successful. Only the humor group decreased depression scores to the preexperimental baseline, although both the humor and waiting groups showed significant decreases in depression scores after the treatment. Zygomatic muscle tension was greater during the humor treatment than other treatments. Heart rate decreased across depression induction for the waiting control only, but increased during the humor and nonhumor treatments. Seven women, who were not included in the above analysis because their initial MAACL depression scores were at least one SD above the mean, showed a paradoxical decrease in depression scores after the depression induction. Implications of the results as they relate to possible interventions for depression are discussed.

Benefits of humor in reduction of threat-induced anxiety.

The benefits of humor in reducing anxiety were investigated in a laboratory study, in which subjects were falsely led to believe that they would receive a shock in 12 min. Participants were 53 undergraduate students with either high or low sense of humor as measured by the Situational Humor Response Questionnaire of Martin and Lefcourt (1984). During the anticipatory period, subjects listened to either a humorous tape, a nonhumorous tape, or no tape. Dependent variables were repeated measures of self-reported anxiety, heart rate, and zygomatic facial activity. Anxiety increased over the anticipatory period, as predicted. A significant interaction between condition and time indicated that subjects from the humor condition consistently rated themselves as less anxious and reported less increase in stress as the shock approached. A three-way interaction concentrating on the final 3 min. indicated a tendency for subjects with low sense of humor to have higher heart rates in the no-tape condition than in the humorous or nonhumorous tape conditions. Analysis for zygomatic activity indicated more smiling by subjects with high sense of humor and by subjects in the humor condition. Also, the humorous tape elicited more smiling by subjects with high sense of humor.

Deaf versus hearing subjects' recall of words on a distraction task as a function of the signability of the words.

Subjects, including 17 deaf and 10 hearing students in Grades 11 and 12, completed a test for memory of lists of 6 words (presented visually for 10 sec.). Subjects recalled the words in writing after a distracting task of adding pairs of digits for 10 sec. Word lists are categorized as signable with a single sign, compound or combination of signs, or finger-spelling signs only. Hearing subjects recalled significantly more words in each category than did deaf subjects. Deaf subjects recalled significantly more single-signed words than either of the other two categories. Deaf subjects did not recall more compound/combination signed words than words that could only be finger-spelled. Hearing subjects also recalled significantly more single-signed words than either of the other two categories and were not superior in either of the other categories.

Depressive and elative mood inductions as a function of exaggerated versus contradictory facial expressions.

Two studies concerned the relation between facial expression cognitive induction of mood and perception of mood in women undergraduates. In Exp. 1, 20 subjects were randomly assigned to a group who were instructed in exaggerated facial expressions (Demand Group) and 20 subjects were randomly assigned to a group who were not instructed (Nondemand Group). All subjects completed a modified Velten (1968) elation- and depression-induction sequence. Ratings of depression on the Multiple Affect Adjective Checklist increased during the depression condition and decreased during the elation condition. Subjects made more facial expressions in the Demand Group than the Nondemand Group from electromyogram measures of the zygomatic and corrugator muscles and from corresponding action unit measures from visual scoring using the Facial Action Scoring System. Subjects who were instructed in the Demand Group rated their depression as more severe during the depression slides than the other group. No such effect was noted during the elation condition. In Exp. 2, 16 women were randomly assigned to a group who were instructed in facial expressions contradictory to those expected on the depression and elation tasks (Contradictory Expression Group). Another 16 women were randomly assigned to a group who were given no instructions about facial expressions (Nondemand Group). All subjects completed the depression- and elation-induction sequence mentioned in Exp. 1. No differences were reported between groups on the ratings of depression (MAACL) for the depression-induction or for the elation-induction but both groups rated depression higher after the depression condition and lower after the elation condition. Electromyographic and facial action scores verified that subjects in the Contradictory Expression Group were making the requested contradictory facial expressions during the mood-induction sequences. It was concluded that the primary influence on emotion came from the cognitive mood-induction sequences. Facial expressions only seem to modify the emotion in the case of depression being exacerbated by frowning. A contradictory facial expression did not affect the rating of an emotion.

Facial electromyogram and heart-rate correlates of a paradoxical attitude change to antinuclear war information.

The effects of film images versus film descriptions of the effects of nuclear explosions (versus a no-film control) on corrugator muscle tension, heart rate, attitude and mood were investigated. The last 5 min. of the images were associated with more corrugator tension for that condition when compared to the last 5 min. of the description condition. The groups did not differ in heart rate but women in both groups showed an increase in heart rate whereas men in both groups showed a decrease in heart rate. Film groups did not differ in their significant increases in anxiety, hostility, and depression on the Multiple Adjective Affect Checklist. On the pretest there was no significant correlation between scores on Betts' Questionnaire Upon Mental Imagery and scores on Goldenring and Doctor's index of concern for nuclear war. The vivid-image film group showed a decrease in concern for nuclear war when compared to the descriptive film group and the no-film control.

Facial action patterns, electromyography, and moods in response to an insoluble task as a function of sex and sex-role differences.

College students of either androgynous or sex-typed orientation were randomly assigned to either an insoluble concept-formation task or a solvable one. Posttreatment scores were compared for measures of dysphoric mood (Multiple Affect Adjective List), electromyographic responses (corrugator and zygomatic), and discrete facial responses (Facial Action Coding System). In Study 1, 18 androgynous women were compared to 16 feminine women; in Study 2, 16 androgynous men were compared to 16 masculine men. The insoluble task was associated with more corrugator activity (frowning) than the solvable task in both studies. Feminine women displayed more corrugator responses across both tasks than androgynous women. However, masculine men did not differ from androgynous men in over-all corrugator response activity. Androgynous women smiled more than feminine women on the facial action coding measure. Men subjected to the insoluble task reported significantly more anxiety, depression, and hostility. Masculine men scored higher on anxiety during the insoluble task than androgynous men, while the latter scored somewhat higher on anxiety in the other condition.

Concordance among self-regulated responses: is self-regulatory ability a psychophysiological "trait"?

The purposes of this study were to examine whether or not self-regulation of physiological responses demonstrates day-to-day reliability, to determine the degree of individual subject consistency (or concordance) in the ability to self-regulate across several different physiological responses, and, finally, to explore the impact of biofeedback training on interresponse concordance. Twenty normal subjects participated in six bidirectional self-regulation sessions-the first and last sessions involving instructions only, and the remainder, biofeedback. Self-regulation scores consisted of the absolute difference between increase and decrease trial means. The average test-retest reliability coefficients (rs) for the self-regulation scores, across the four biofeedback sessions, were a highly significant .50, .68, .30, and .47 for EEG, EMG, HR, and SCL, respectively. By contrast, the average concordance among the self-regulation scores for the four feedback sessions, estimated by Kendall's coefficient of concordance, was a marginally significant 39% of the possible variance of the rank sums. This corresponds to an average between-response rs value only of .19. The concordance level from the initial no-feedback (i.e., instructions only) session was not significant. Multivariate concordance levels did increase during the first three feedback sessions, but declined at the fourth, and again was nonsignificant during the final no-feedback session. Among the individual self-regulation response pairings, only the EEG/EMG combination was consistently associated during the no-feedback sessions. The present results suggest that self-regulatory ability is neither a highly unitary "trait"-like phenomenon nor an entirely response-specific event, but may vary considerably as a function of subject factors, or the situational circumstances, under which it is measured.

Intestinal degradation and absorption of the glycosidic sweeteners stevioside and rebaudioside A.

Contrary to prior indications, the glycosidic sweeteners stevioside and rebaudioside A are degraded to the diterpenoid aglycone steviol by rat intestinal microflora in vitro. Additional studies with steviol-17-[14C] show almost total absorption from the rat lower bowel following intracecal administration.