ABSTRACT
The main furanocoumarins from the roots of PEUCEDANUM PALUSTRE were separated with a RP-HPLC method. The mobile phase for the separation of the eight main compounds (oxypeucedanin hydrate, bergapten, oxypeucedanin, isobyakangelicin angelate, ostruthol, imperatorin, isoimperatorin, and columbianadin) was optimized with the "PRISMA" model, which is a multi-solvent optimization system. The chromatographic method can be applied for quantitative determination, as was demonstrated for different samples of P. PALUSTRE. The purity and identity of the peaks were controlled by diode-array detection and comparison with standards.
ABSTRACT
The main flavonoid glycosides from BETULAE folium are separated with a RP-HPLC method. The mobile phase for the separation of the seven most important compounds (rutin, hyperin, quercetin-3-arabinofuranoside, quercetin-3-arabinopyranoside, quercetin-3-rhamnoside, quercetin-3-glucuronide, and myricetin-3-galactoside) was optimized with the "PRISMA" model, which is a multisolvent optimization system. The isocratic separations were finished within 30 minutes and the peak purity was controlled with diode-array detection. The method can be applied for quantitative determination, as was demonstrated for different samples of B. PENDULA and B. PUBESCENS.
