Association of different sleep characteristics and cardiometabolic risk in college students / 中国学校卫生

Objective@#To describe the association of different sleep characteristics and cardiometabolic risk among college students, so as to provide reference for health promotion of college students.@*Methods@#By random cluster sampling method, a questionnaire survey and physical examination including blood pressure, waist circumference and blood lipid indicators, which were conducted in April and May of 2019 among a total of 1 179 college students from the first grade in two universities in Hefei City of Anhui Province and Shangrao City of Jiangxi Province. A total of 729 college students with valid questionnaires were included into analysis. The Pittsburgh Sleep Quality Index (PSQI) and Insomnia Severity Index (ISI) were used to investigate sleep behavior, and the Morning And Evening Questionnaire-5 (MEQ-5) was used to investigate sleep characteristics. The cardiometabolic risk score was derived using the sum of the standardized sex specific Z scores of waist circumference, mean arterial pressure, HDL cholesterol (multiplied by -1), triglycerides, and insulin resistance index. The rank sum tests were used to compare differences in cardiometabolic risk scores across demographic characteristics. Generalized linear models were used to compare the association of different sleep characteristics with cardiometabolic risk scores among college students.@*Results@#The average cardiovascular metabolic risk score of college students was -0.32(-2.03, 1.58). There were statistically significant differences in cardiovascular metabolic risk scores among college students in variables such as smoking, health status, and physical activity levels ( t/F=-3.41, 12.88, 51.07, P <0.01). The results of the generalized linear model showed that nighttime preference ( B=1.89, 95%CI =1.02-3.49), insomnia symptoms ( B=3.25, 95%CI =1.79-5.90), and short or long sleep duration ( B=1.92, 95%CI =1.21-3.05) were positively correlated with the cardiovascular metabolic risk score of college students ( P <0.05).@*Conclusions@#Poor sleep patterns among college students are positively correlated with the risk of cardiovascular metabolism. The sleep behavior of college students should be actively changed to reduce the risk of cardiovascular disease.

Establishment of a reference interval for total carbon dioxide using indirect methods in Chinese populations living in high-altitude areas: A retrospective real-world analysis.

BACKGROUND: Hypoxia leads to different concentrations of the bicarbonate buffer system in Tibetan people. Indirect methods were used to establish the reference interval (RI) for total carbon dioxide (tCO2) based on big data from the adult population of Tibet, a high-altitude area in Western China. METHODS: Anonymous tCO2 test data (n = 442,714) were collected from the People's Hospital of the Tibet Autonomous Region from January 2018, to December 2021. Multiple linear regression and variance component analyses were performed to assess the effects of sex, age, and race on tCO2 levels. Indirect methods, including Hoffmann, Bhattacharya, expectation maximization (EM), kosmic and refineR, were used to calculate the total RI and ethnicity-partitioned RI. RESULTS: A total of 230,821 real-world tCO2 test results were eligible. Sex, age, and race were significantly associated with the tCO2 levels. The total and ethnically-partitioned RIs estimated using the five indirect methods were comparable. The total RI of tCO2 was 14-24 mmol/L (calculated using Hoffmann and refineR) and 15-24 mmol/L (Bhattacharya, EM and kosmic). For Han nationality, the RIs were 14-25 mmol/L (calculated using Hoffmann and Bhattacharya), 16-23 mmol/L (EM), 15-24 mmol/L (kosmic), and 14.2-24.5 mmol/L (refineR). For the Tibetan population, the RIs were 14-24 mmol/L (calculated using Hoffmann and refineR), 15-24 mmol/L (Bhattacharya and kosmic), and 15-23 mmol/L (EM). The established RIs were significantly lower than those living at lower altitudes area (22-29 mmol/L) that was provided by the manufacturer. CONCLUSION: The tCO2 RI of the populations living on the Tibetan Plateau was significantly lower than those at the lower altitudes. The RIs established using indirect methods are suitable for clinical applications in Tibet.

The Association between Neprilysin gene polymorphisms and Alzheimer's disease in Tibetan population.

OBJECTIVES: Alzheimer's disease (AD) is a well-known neurodegenerative disease, of which the hallmark is the disposition of ß-amyloid (Aß) in the form of plaque in the brain. Neprilysin (NEP) is the major enzyme to degrade Aß and prevent accumulation of Aß. The present study was undertaken to elucidate the correlation between the NEP gene polymorphisms and AD in Chinese Tibetan population. METHODS: Ninety-nine sporadic AD Tibetan patients and 113 healthy Tibetan controls were enrolled in this study. The genotype frequencies and allele frequencies of multiple NEP gene loci were analyzed using the case-control association analysis. RESULTS: No significant correlation was found between polymorphisms of NEP gene loci (rs9829757, rs1816558, rs6776185, rs3736187, rs701109, rs989692) and the occurrence of AD in Tibetan population. However, allele C of NEP gene locus (rs701109) and allele T of gene locus (rs3736187) were possible risk factors of male AD patients in Tibetan population. CONCLUSIONS: NEP gene loci (rs701109, rs989692, rs9829757, rs3736187, rs1816558, rs6776185) were polymorphic in Tibetan population. No difference was found between these loci but for that male gender combined with allele C of NEP gene locus (rs701109) and T of gene locus (rs3736187) might be risk factors for AD in Tibet.

Design and development of pathway management system for nucleusrelated special service recuperation / 医疗卫生装备

Objective To develop a pathway management system for nucleus-related special service recuperation to enhance efficiency and extend function in the pathway management.Methods The system was developed on the basis of military sanatorium information management platform,and constructed with modular and object-oriented method,B/S mode,Oracle 8 database and Java programming languages.Results The system solved the problems of paper pathway management in time consumption,supervision,statistics,regulation and etc.Conclusion The system contributes to the standardization,convenience and precision of the pathway management for nucleus-related special service recuperation,and thus is worthy promoting in the nucleus-related military sanatorium.

[Spatial pattern analysis and associations of Quercus aquifolioides population at different growth stages in Southeast Tibet, China].

This article analyzed the spatial pattern and its correlation of Quercus aquifolioides, Southeast Tibet at different growing stages by using Ripley' s L function in the method of point pattern, analysis. The results showed the diameter structure of Q. aquifolioides population in Southeast Tibet followed a 'single peak' shape and the saplings and medium trees predominated in number in the whole population. The population had a high regeneration rate and was of increase type. In the growth process of Q. aquifolioides from saplings to large trees, saplings and medium trees showed aggregation distribution at.small scale, while large trees showed basically random distribution at whole scale. There was significant correlation between saplings with medium or large trees at small scale, however, there was no correlation between medium and large trees. In the growth process of Q. aquifolioides population from saplings, medium trees to large trees, its spatial pattern developed from aggregative distribution to random distribution. The natural regeneration of Q. aquifolioides population was affected not only by interspecific competition, but also by intraspecific competition. In the similar natural environment, the most important factors affecting the spatial pattern of Q. aquifoioides population were its own biological and ecological characteristics.

Effect of ozonized saline on signaling passway of Keap1-Nrf2-ARE in rat hepatocytes / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To study the effect of ozonized saline on the activation of the Keap1-Nrf2-ARE signaling pathway in rat liver cells.</p><p><b>METHODS</b>Twenty male Sprague-Dawley rats were randomly divided into ozonized saline (OS) group, model group, ozonized saline control (OSC) group and normal control (NC) group. The rats in OS group and model group were intravenously administered with OS or oxygen saline (5 ml/kg) respectively, once a day for 15 days, and then intraperitoneally injected with CCl4 dissolved in oliver oil. The rats in OSC group were pretreated with OS for 15 days. The rats in NC group were fed normally for 15 days. On the 16th day, the rats in OSC group and NC group were intraperitoneally injected with oliver oil (2 ml/kg) without CCl4. After 24 hours of CCl4 or olive oil intraperitoneal injection, the serum levels of alanine transaminase (ALT) and aspertate aminotransferase (AST) were measured. The liver tissues were also collected for detection of total anti-oxygen capability (TAOC), glutathione (GSH), catalase (CAT), Glutathione peroxidase (GPx). Western Blot was used to detect Nrf2 and immunofluorescence staining assay to display intracelluar distribution of Nrf2.</p><p><b>RESULTS</b>Compared with the rats in model group,the serum ALT and AST levels of rats in OS group were significantly lower (P < 0.01) ,which were (1240.4 ± 188.2) U/L and (1245.4 ± 176.9) U/L vs (539.8 ± 175.3) U/L and (546.0 ± 130.2) U/L, and the TAOC, CAT, GPx and GSH activity of rats in OS group were significantly higher, which were (0.72 ± 0.24) U/mg, (1.05 ± 0.21) mg/g, (676.9 ± 115.1) U/mg and (45.2 ± 14.3) U/mg vs (1.37 ± 0.19) U/mg, (2.23 ± 0.55) mg/g, (1024.6 ± 162.9) U/mg and (68.2 ± 9.9) U/mg, respectively. In contrast with NC group, pretreatment of OS in OSC group elevated TAOC, CAT, GPx and GSH activity (P < 0.01 or P < 0.05). Ozonized saline can strengthen the Nrf2 expression in liver cells.</p><p><b>CONCLUSION</b>Preconditioning injection of ozonized saline can reduce rat's liver injury induced by CCl4. The ozonized saline, as a novel Nrf2 activator, can reduce the oxidative damage of radical oxygen species (ROS) and the deleterious substance by activating the Keap1-Nrf2-ARE signaling pathway and its downstream genes expression.</p>

Study on the tongue manifestations for the blood-stasis and toxin syndrome in the stable patients of coronary heart disease / 中国结合医学杂志

<p><b>OBJECTIVE</b>To explore the tongue manifestations for the blood-stasis and toxin syndrome in the stable patients of coronary heart disease (CHD) according to the acute cardiovascular events (ACEs) in one-year follow-up which based on the pathogenesis hypothesis of "blood-stasis and toxin causing catastrophe".</p><p><b>METHODS</b>Totally 254 stable CHD cases were enrolled after diagnosed by coronary angiography, their tongue appearances were recorded by the digital camera of uniform type, 29 cases with ACEs during one-year follow-up were assigned in ACEs group. The non-ACEs were matched in proportion of 2:1 according to the gender, age (±2.5 years), diabetes mellitus history and previous acute coronary syndrome hospitalization history in the non-ACEs group, and 54 cases were eligibly included. The differences of tongue appearance between the ACEs and non-ACEs group were compared.</p><p><b>RESULTS</b>Fifteen cases manifested with bluish tongue (including bluish-grounding or bluish purple tongue), among which 11 cases (37.9%) in the ACEs group and four cases (7.4%) in the non-ACEs group, and there was significant difference (P<0.002). Twenty six cases showed yellow tongue coating in the non-ACEs group, which was significantly higher than that in the ACEs group (48.1 vs. 10.3%, P=0.001). The tongue of sticky greasy coating was more frequently occurred in the non-ACEs group than that in the ACEs group (66.7% vs. 41.4%, P=0.026). The proportion of purplish-red sublingual vessel was higher in the ACEs group than that in the non-ACEs group (41.4% vs. 20.4%, P=0.041). Odd ratio (OR) analysis showed that the patients with bluish tongue, purplish-red sublingual vessel, dry-greasy or dirty greasy coating were more likely to experience ACEs during one-year follow-up (OR: 11.67, 95%CI: 3.34 year 3.34-40.81, P<0.001; OR: 2.76, 95%CI: 1.02 1.02-7.44, P<0.05; OR: 3.12, 95%, CI: 0.89 0.89-10.92, P=0.066).</p><p><b>CONCLUSIONS</b>The bluish tongue (including bluish-grounding or bluish purple tongue) and purplish-red sublingual vessel were potential tongue manifestations of blood-stasis and toxin. The tongue coating changing from sticky greasy to dry greasy or dirty greasy was also probably a tongue manifestation of "transforming toxin", which need demonstration by further study.</p>

Anti-apoptosis effect of tremella polysaccharides on cardiomyocytes / 第二军医大学学报

Objective: To observe the anti-apoptosis effect of tremella polysaccharides (TP) on cardiomyocytes by in vitro and in vivo experiments. Methods: TP was extracted by dehydrated alcohol and the TP purity was measured by gas chromatography. The cultured cardiomyocytes of the neonatal rats were randomly assigned to normal group (Group A), apoptosis-induced group (Group B) and TP-pretreatment group (Group T). The morphology and uptake rate of trypan blue were studied after 72 h. The apoptosis index of cardiomyocyte was measured by flow cytometry. In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks. Results: Data of cultured cardiomyocytes were as follows: Uptake rate of trypan blue: B>C>A (PC> A (P<0.01). In the animal studies there were no obvious difference in myocardium pathology between groups. The order of the apoptosis indices of myocardium was H

Analysis of relationships among syndrome, therapeutic treatment, and Chinese herbal medicine in patients with coronary artery disease based on complex networks / 中西医结合学报

To analyze the relationships among syndrome, therapeutic method and Chinese herbal medicine in patients with coronary artery disease (CAD).

CT features of adult mycoplasma pneumoniae pneumonia / 中国医学影像技术

Objective To observe the CT features of mycoplasma pneumoniae pneumonia. Methods CT features of 40 adult patients with serologically proven mycoplasma pneumoniae pneumonia were retrospectively reviewed. Results The most common CT findings of mycoplasma pneumoniae pneumonia were ground-glass opacity (34/40, 85.00%) and consolidation (28/40, 70.00%). The other findings included nodules (18/40, 45.00%), thickening of the bronchovascular bundles (6/40, 15.00%), interlobular septal thickening (6/40, 15.00%), mediastinal lymphadenopathy (6/40, 15.00%) and pleural effusion (4/40, 10.00%). Most of the ground-glass opacity and consolidation showed tendency of lobular distribution and involving the lower lobes. In some patients, the absorption of the lesions was slower than the relief of symptoms. Conclusion CT findings of mycoplasma pneumoniae pneumonia have some features, and can provide help for the diagnosis of mycoplasma pneumoniae pneumonia.

Clinical manifestations of "blood-stasis and toxin" in patients with stable coronary heart disease / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To explore the clinical manifestations of "blood-stasis and toxin" (BST) in patients with stable coronary heart disease (CHD) on the basis of "BST Causing Catastrophe" hypothesis, by analyzing the occurrence of acute cardiovascular events (ACEs) in a one-year follow-up period.</p><p><b>METHODS</b>Two hundred and fifty-four CHD patients in stable stage, whose diagnosis confirmed by coronary arteriography, were followed-up for one year with their clinical data recorded detailedly. The relationship between the occurrence of ACEs and personal history, body constitution, past history of illness, family history, clinical symptoms, physical signs, syndrome types and laboratory indexes were analyzed by single-variate and multivariate logistic stepwise regression method.</p><p><b>RESULTS</b>During the follow-up period, two cases out of the 254 patients were lost, no case of death or acute myocardial infarction was seen, three cases underwent percutaneous coronary intervention, and 25 cases were hospitalized for unstable angina. Multivariate logistic regression analysis showed that hesitant or intermittent pulse, retrosternal pain, usual pharyngodynia and headache were the most important influencing factors for occurrence of ACEs (P < 0.05). High-sensitivity C-reactive protein (hs-CRP) > or =3 mg/L also indicated a predictive trend for ACEs occurrence, although logistic analysis showed no statistics difference (P = 0.094).</p><p><b>CONCLUSION</b>Retrosternal pain, headache, hesitant or intermittent pulse, usual pharyngodynia and increased hs-CRP might be taken as the clinical manifestations of BST, which provides a basis for applying Chinese medicine intervention (activating blood circulation and detoxifying) on high-risk CHD patients. Further demonstration is needed.</p>

Osteoblastic differentiation and gene expression profile change in rat bone marrow mesenchymal stem cells after a single period of mechanical strain / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the osteoblastic differentiation and compare the difference in the gene expression of rat bone marrow mesenchymal stem cells (MSCs) affected by a single period of mechanical strain.</p><p><b>METHODS</b>Bone marrow MSCs were harvested from the femurs and tibiae of SD rats and cultured in vitro. A four-point bending apparatus were used to perform a single 40-minute period of 2,000 microepsilon mechanical strain on these MSCs. The proliferation of the MSCs was tested by MTT on scheduled date, and the osteoblastic differentiation of the MSCs was measured by testing the expression of osteocalcin and alkaline phosphate (ALP) activity of these cells. In addition, we have investigated the possible mechanisms underlying the action of the single 40-minute period of 2,000 microepsilon mechanical strain on these MSCs, after profile blotted and handled by bioinformation, the gene expressions of these two periods of MSCs were examined.</p><p><b>RESULTS</b>The MSCs have grown well in vitro. Our experiment showed that mechanical environment did not weaken the proliferation of the MSCs. However, the ALP activity and the expression of osteocalcin were significantly up-regulated by the 2,000 microepsilon mechanical strain. Using the 27 K Rat Genome Array, 416 different expressions were found. The rate of different genes was 2.8%, of which the expressions of 247 genes increased (61 genes remarkably increased) and 169 genes decreased (74 genes remarkably decreased) in these two periods of MSCs.</p><p><b>CONCLUSION</b>Mechanical strain induced the osteoblastic differentiation of the MSCs, which may be attributed to the different gene levels.</p>

Effect of Tremella Polysaccharides on cardiomyocytes in vitro and in vivo / 中华临床营养杂志

@#myocytes.In aging mice induced by D-galactose experiment.TP has anti-apoptosis and anti-oxidation effect in a dose-dependant manner.

Effect of tremella polysaccharides on the antioxidant ability of experimental aging mice / 中华临床营养杂志

ificantly lower than in both middle and low group(P＜0.05).Conclusion TP may improve the antioxidant ability of experimental aging mice.

Relationship between Chinese medicine syndrome and serum level of high-sensitivity C-reactive protein in patients with stable coronary heart disease / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the relationship between Chinese medicine syndrome and serum level of high-sensitivity C-reactive protein (hs-CRP) in patients with stable coronary heart disease (CHD).</p><p><b>METHODS</b>Serum hs-CRP level was determined in 346 patients with stable CHD, whose diagnoses were confirmed by coronary angiography, and the correlation between patients' syndrome and serum hs-CRP level was analyzed.</p><p><b>RESULTS</b>In all the CHD patients enrolled, the most commonly encountered Chinese medicine complex syndromes were the qi deficiency and blood-stasis syndrome, the yang deficiency and blood-stasis syndrome, and the qi deficiency with blood-stasis and turbid phlegm syndrome; the dominant syndrome types were blood-stasis, qi deficiency and phlegm-turbidity, which revealed in 324 patients (93.6%), 189 patients (54.6%) and 140 patients (40.5%), respectively. Comparisons of hs-CRP level between different complex syndromes and syndrome elements showed no significant difference (P > 0.05). However, for patients with phlegm-turbidity syndrome, the hs-CRP level was much higher in patients tended to heat than in those tended to cold (2.23 +/- 2.12 mg/L vs 1.59 +/- 1.27 mg/L, P < 0.05). Besides, the score of blood-stasis syndrome showed no correlation with hs-CRP level (Person correlation coefficient: 0.069, P = 0.203).</p><p><b>CONCLUSION</b>The elevation of hs-CRP level is closely cor related with phlegm-heat syndrome in stable CHD patients. As an inflammatory biomarker, hs-CRP may be regarded as one of the microcosmic indices of toxin in Chinese medicine, which provides an objective evidence for the pathogenesis hinge of accumulated heat to transform toxin, so it is worthy of further study.</p>

Effect of micronutrients on pressure ulcer / 肠外与肠内营养

The effect of micronutrients on pressure ulcer was reviewed.Nutritional treatment is helpful to prevent and cure the pressure ulcer.Especially micronutrients should be paid more attention to.