ABSTRACT
1. The concentrations of interleukin 1 (IL-1) and interleukin 2 (IL-2) produced in the supernatants of peripheral blood mononuclear cell cultures from patients with advanced cancer were measured to identify some of the causes of the immunological impairment characteristic of malignant disease. 2. Mononuclear cells obtained from 19 cancer patients were stimulated to produce IL-1 and IL-2 and compared with those of healthy controls. A severe reduction of both IL-1 and IL-2 activity was observed. 3. There was no correlation between the lower number of OKT4+ cells observed in these patients and the levels of IL-2 production. The removal of monocytes did not bring IL-2 levels to normal. Impaired IL-2 production could not be restored to normal by addition of IL-1. 5. These results suggest that exogenous IL-1 and IL-2 may be useful in cancer immunotherapy.
Subject(s)
Interleukin-1/biosynthesis , Interleukin-2/biosynthesis , Leukocytes, Mononuclear/metabolism , Neoplasms/blood , Adult , Female , Humans , Immune Tolerance , Leukocytes, Mononuclear/analysis , Lymphocyte Activation , Male , Middle Aged , Neoplasms/immunology , Neoplasms/pathology , Phytohemagglutinins/pharmacology , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
The concentrations of interleukin 1 (IL-1) and interleukin 2 (IL-2) produced in the supernatants of peripheral blood mononuclear cell cultures from patients with advanced cancer were measured to identify some of the causes of the immunological impairment characteristic of malignant disease. Mononuclear cells obtained from 19 cancer patients were stimulated to produce IL-1 and IL-2 and compared with those of healthy controls. A severe reduction of both IL-1 and IL-2 activity was observed. There was no correlation between the lower number of OKT4+ cells observed in these patients and the levels of IL-2 production. The removal of monocytes did not bring IL-2 levels to normal. Impaired IL-2 production could not be restored to normal by addition of IL-1. These results suggest that exogenous IL-01 and IL-2 may be useful in cancer immunotherapy
Subject(s)
Adult , Middle Aged , Humans , Male , Female , Interleukin-1/biosynthesis , Interleukin-2/biosynthesis , Leukocytes, Mononuclear/metabolism , Neoplasms/immunology , Kidney Neoplasms/immunology , Leukocytes, Mononuclear/analysis , Lung Neoplasms/immunology , Colonic Neoplasms/immunology , Neoplasms/pathology , Phytohemagglutinins/pharmacology , Tetradecanoylphorbol Acetate/pharmacology , Lymphocyte Activation , Urinary Bladder Neoplasms/immunology , Uterine Cervical Neoplasms/immunologyABSTRACT
Guanylate cyclase (GCase) and cyclic guanosine 3'5'-monophosphate-phosphodiesterase (cGMP-PDE) activities were measured in the medulla, glomeruli and cortical tubules isolated from the remaining kidney 5-120 min after unilateral nephrectomy. The results showed that cGMP-PDE-specific activities were unchanged in all the fractions isolated from the contralateral kidney when compared with the nephrectomized control kidney. The GCase-specific activity in the cortical and medullary tubules isolated from the remaining kidney reached a maximum by 10 min after unilateral nephrectomy and then returned to control values. On the other hand the glomerular GCase activity decreased for 10 min after unilateral nephrectomy, then rebound activity reached a maximum by 30 min and after 120 min had returned to the control value. The possible physiological signification of such variations is discussed.
