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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 27(5): 494-7, 2011 May.
Article in Chinese | MEDLINE | ID: mdl-21557903

ABSTRACT

AIM: To stably express herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) in Chinese hamster ovary cells (CHO-K1). METHODS: The eukaryotic expression vector pCI-mCMV-gC-1-IRES-DHFR-L22R was constructed and transfected into CHO-K1 cells by Lipofectamine 2000. The transfected cells were selected by G418 and methotrexate (MTX). The expression of HSV-1 gC was analyzed by Slot blot. HSV-1 gC proteins were purified with His-Ni Sepharose and then detected by Western blot. RESULTS: The eukaryotic expression vector pCI-mCMV-gC-1-IRES-DHFR-L22R was constructed successfully. CHO-K1 cells stably expressing HSV-1 gC proteins were established and confirmed by Western blot. CONCLUSION: The HSV-1 gC proteins have been expressed successfully and have good bioactivity. The results make it possible for further study and clinical use of HSV-1 gC.


Subject(s)
Genetic Vectors/genetics , Viral Envelope Proteins/genetics , Animals , CHO Cells , Cloning, Molecular , Cricetinae , Cricetulus , Genetic Vectors/biosynthesis , Genetic Vectors/chemistry , Viral Envelope Proteins/biosynthesis
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