ABSTRACT
Immunochemical study of blood sera from mice immunized with rabbit immunoglobulins (AT1) to herpes simplex virus type I (HSV-I) detected antiidiotypic antibodies AT2 in five of them (antiID). The affinity-purified antiID were shown to induce in syngeneic mice production of antibody (AT3) interacting with HSV-I antigen. In vitro, antiID (AT3) were shown to neutralize the virus activity. Analysis of the results suggests that the antiID imitate the antigenic properties of HSV-I and can induce specific immune response.
Subject(s)
Antibodies, Anti-Idiotypic/immunology , Antibodies, Viral/immunology , Simplexvirus/immunology , Animals , Antibodies, Anti-Idiotypic/blood , Antibodies, Anti-Idiotypic/isolation & purification , Antibodies, Viral/blood , Antibodies, Viral/isolation & purification , Chromatography, Affinity , Complement Fixation Tests , Immunization , Immunoenzyme Techniques , Mice , Mice, Inbred BALB C , Neutralization Tests/methods , Rabbits , Simplexvirus/pathogenicitySubject(s)
Herpes Genitalis/diagnosis , Herpes Simplex/diagnosis , Pregnancy Complications, Infectious/diagnosis , Antibodies, Viral/blood , Antibodies, Viral/cerebrospinal fluid , Complement Fixation Tests , Evaluation Studies as Topic , Female , Fluorescent Antibody Technique , Herpes Simplex/congenital , Humans , Immunoenzyme Techniques , Infant, Newborn , Pregnancy , Recurrence , Simplexvirus/immunologyABSTRACT
Under conditions of immunosuppression of B- and T- cell function by cyclophosphane the vaccination process in mice immunized with vaccinia virus was considerably more severe than in the untreated animals: mortality in immunosuppressed mice was much higher than in the controls; the virus could be isolated from their lungs for longer periods and in higher titres; the number of antibody-producing cells in the spleen and antibody levels in the blood were lower. In contrast to the inactivated vaccine, live vaccine itself exerted a marked immunosuppressive effect.
Subject(s)
Cyclophosphamide/immunology , Immunosuppression Therapy/methods , Vaccinia virus/immunology , Variola virus/immunology , Animals , Antibody Formation/drug effects , Immunity, Cellular/drug effects , Immunization , Mice , Time Factors , Vaccines, Attenuated/immunologyABSTRACT
The hemolytic plaque test in mice was used to demonstrate that live vaccinia virus has marked immunosuppressant action. Inactivated vaccinia virus produces no such an effect and even stimulates the output of the antibody-forming cells. The experimental data were found to be statistically significant.
