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J Agric Food Chem ; 63(20): 4999-5007, 2015 May 27.
Article in English | MEDLINE | ID: mdl-25959257

ABSTRACT

Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEO(f+l) and AKEO(stm), respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-ß-ocimenes, methyleugenol, limonene, spathulenol, ß-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (<0.1%) amounts in one or both plant components. Screening of the essential oils for biological activity showed that AKEO(stm), but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca(2+) flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEO(stm) composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca(2+) flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca(2+) flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.


Subject(s)
Artemisia/chemistry , Neutrophils/drug effects , Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Plant Oils/pharmacology , Cell Movement/drug effects , Gas Chromatography-Mass Spectrometry , HL-60 Cells , Humans , Neutrophils/cytology , Neutrophils/immunology , Oils, Volatile/chemistry , Plant Extracts/chemistry , Plant Leaves/chemistry , Plant Oils/chemistry
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