Effects of mechanochemically activated doxorubicin and 40 MHz frequency irradiation on human A-549 lung carcinoma cells.

AIM: To study in vitro influence of mechanochemically activated (MA) doxorubicin (DOXO) and electromagnetic irradiation (EMI) on human lung carcinoma A-549 cells. METHODS: Solid state DOXO was MA by input energy 20 W/g during 5 min. Tumor cells were exposed to 40 MHz EMI with power density 2 W/cm(2) at temperature 37 degrees C. RESULTS: Particles of MA DOXO have sizes 10 time smaller than officinal DOXO, high performance liquid chromatography analysis showed that parameters of officinal and MA DOXO were quantitatively equal. Mechanochemical activation initiated in the drug formation of free radicals with g = 2.005, g = 2.003 and g = 1.97. LD(50) values of MA DOXO were 5 times lower than that of officinal drug. Cell survival decreased in the following way after effects EMI --> officinal DOXO --> MA DOXO --> officinal DOXO + EMI --> MA DOXO + EMI. CONCLUSION: Treatment by MA DOXO and drug with EMI at 37 degrees C showed better targeting of drug in human lung carcinoma A-549 cells outcomes than officinal DOXO.

Mechanoluminescence: lymphocyte analysis after exposure to ionizing radiation.

Lymphocyte mechanoluminescence (ML) develops as a result of mechanochemical activation of cells. This paper describes devices (tribogenerators) and techniques for lymphocyte mechanoluminescence analysis. At different stages of the cellular cycle the ML of non-irradiated peripheral blood lymphocytes (PBL) showed characteristic differences. After (60)Co gamma -irradiation of PBL in vitro at 0.25 Gy and 1 Gy an alteration of ML was observed with a relationship between ML values of lymphocytes, and also of mitochondria (MT), and the dose of ionizing radiation used. By using bioluminescence techniques it was shown that gamma-irradiation reduces the amount of ATP synthesized by PBL in the stationary phase.

[The effect of gamma irradiation on lymphocyte mechanical emission in rats at different stages of the cell cycle]. / Vliianie gamma-oblucheniia ba mekhanoémissiiu limfotsitov krys v razkykh stadiiakh kletochnogo tsikla.

Mechanoemission (ME) of rat peripheral blood lymphocytes (PBL) over the radio-frequency range has characteristic peculiarities in various stages of the cell cycle, both in unirradiated cells and in cells exposed to 0.25 or 1 Gy of gamma radiation. After 52 h incubation of irradiated and unirradiated lymphocytes, ME PBL indices take values equivalent to initial ones in the G0 phase. Increase of radiation dose from 0.25 to 1 Gy was followed by a decrease of the value of the ME PBL kinetic parameter after 24, 40 and maximum 48 h incubation.

[Structural dynamic properties of the cell membranes of Ehrlich ascites tumor associated with a polyamine deficiency caused by DL-difluoromethylornithine]. / Strukturno-dinamicheskie svoistva membran kletok astsitnogo raka Erhlikha pri defitsite poliaminov, vyzvannom DL-diftormetilornitinom.

The blocking of polyamine synthesis in cancer cell by a specific inhibitor (DFMO) results in an increase in membrane viscosity and in significant changes in cell deformability as determined by cell penetration through a nuclear filter with given porosity. Almost two-fold reduction of the penetration rate through the filter pores was registered for the DFMO-treated cells in spite of the fact that the cell size decreases after such a treatment. The number of cells penetrating through the filter after DFMO treatment is diminished due to an increasing resistance to penetration. The addition of putrescine to the incubation medium at physiological concentration restores all these parameters to the initial values. The possible association of these changes with differential sensitivity of cancer cells towards the inhibitor action depending on the stage of cell cycles is discussed.

[Effect of preparations of unsaturated fatty acids on the lipid component of the endoplasmic reticulum membranes of liver cells in rats following administration of N-nitrosodiethylamine]. / Vliianie preparatov nenasyshchennykh zhirnykh kislot na lipidnyi komponent membran éndoplazmaticheskogo retikuluma kletok pecheni krys pri vvedenii N-nitrozodiétilamina.

The effect of drugs, including unsaturated fatty acids and antioxidants, on the lipid composition of the endoplasmic reticulum membranes was studied at early stages of NDEA-induced hepatocarcinogenesis. The complexes containing oleic, linoleic, and arachidonic acids and biological antioxidants prevent changes in the fatty acid composition and the lipid bilayer organization of the both membrane fractions of endoplasmic reticulum which are observed after the injection of NDEA alone, and they inhibit the appearance and growth in the liver of gamma-glutamyltranspeptidase positive hyperplastic nodules.

[Effect of modification of the lipid bilayer on the functioning of the electron transport chain of endoplasmic reticulum membranes in nitrosodiethylamine-induced hepatic carcinogenesis]. / Vliianie modifikatsii lipidnogo bisloia na funktsionirovanie élektrontransportnoi tsepi membran éndoplazmaticheskogo retikuluma pri gepatokantserogeneze, indutsirovannom nitrozodiétilaminom.

The kinetics of cytochrome P-450 content in endoplasmic reticulum membranes at different stages of hepatocarcinogenesis was studied under the influence of unsaturated fatty acids by the EPR method. At early stages of carcinogenesis the preparations containing oleic, linoleic and arachidonic acids prevent changes in the cytochrome P-450 content usually observed in case of nitrosodiethylamine introduction. The diet containing unsaturated fatty acids has a stabilizing effect revealed by the similar changes in the content of cytochrome P-450, phosphatidyl choline and fatty acids composition and also in the level of organization of the endoplasmic reticulum membrane lipid bilayer.

[Fatty-acid composition of lipids and physicochemical characteristics of membrane fractions and the membrane of endoplasmic reticulum of thymus and Pliss' lymphosarcoma]. / Zhirnokislotnyi sostav lipidov i fiziko-rhimicheskie kharakteristiki membrannykh fraktsii timusa i limfosarkomy Plissa.

The paper is concerned with composition of neutral lipids and phospholipids, the regularity of lipid bilayer and structural reorganization of plasma membranes, and membranes of smooth and rough cell reticulum of thymus and Pliss lymphosarcoma are studied at linear and stationary growth phase. No qualitative differences are found in the fatty-acid composition of lipid membranes in normal and tumour cells. In plasma membranes of phospholipids and in membranes of smooth reticulum of tumour cells the unsaturated lipid component increases in the process of growth, the cholesterin/phospholipids ratio decreases, fluidity of the lipid bilayer diminishes and structural heterogeneity of these membranes rises while in membranes of rough reticulum the saturation of lipids increases, but the cholesterin/phospholipids ratio does not change. The temperatures of structural reorganization also does not change, which evidences for a less structural lability of membranes of rough reticulum as compared with other membranes.

[Oxidative free radical processes and bioantioxidants during viral carcinogenesis]. / Okysni vil'noradykal'ni protsesy ta bioantyoksydanty za virusnoho kantserohenezu

The changes in biochemiluminescence intensity of tissue homogenates, tissue lipids as well as the values of the antioxidative activity of the free-radical processes regulators, bioantioxidants of lipids, and their antiradical activity were studied in viral carcinogenesis induced by Moloney's oncornavirus. Kinetics of biochemiluminescence of muscles homogenates and muscular tissue tumours (Moloney's sarcoma) and that of chemiluminescence of total lipids extracted from the muscular and tumour tissue were studied as well. A sharp increase in the intensity of lipids biochemiluminiscence and decrease in the tissue homogenates biochemiluminiscence were observed during the period of progressive tumour growth on the 6-8 days following introduction of the virus. At the same time the values of the antioxidative activity and antiradical activity of the tissue lipids bioantioxidants were minimal. Results of this investigation suggest that the expenditure of bioantioxidants are rather active during the period of progressive tumour growth. The regularities of the changes in biochemiluminescence intensity in the muscle tissue homogenates with viral carcinogenesis cannot be explained exceptionally by an increase in the amount of bioantioxidants in the tumour tissue lipids. Evidently the peculiarities of lipid-protein interactions might play an essential role in the mentioned process.