1.
J Immunol Methods
; 10(2-3): 127-32, 1976 Mar.
Article
in English
| MEDLINE
| ID: mdl-58938
ABSTRACT
Spleen cells from mice immunized with E. coli beta-galactosidase were incubated with the enzyme and with 5-bromo-4-chloroindolyl-beta-galactosidase as a histochemical substrate. Some cells are stainable in this reaction, however, not all of them carry specific receptors for beta-galactosidases. Some spleen cells can take up dye and thereby become unspecifically stained. This system is therefore not suitable for the detection of antigen binding to cells.
Subject(s)
Galactosidases/immunology , Lymphocytes/analysis , Spleen/cytology , Animals , Binding Sites, Antibody , Lymphocytes/immunology , Mice , Staining and Labeling
2.
Z Zellforsch Mikrosk Anat
; 113(4): 581-608, 1971.
Article
in German
| MEDLINE
| ID: mdl-4250828