ABSTRACT
The nutrient medium on the basis of enzymatic hydrolysate of rice flour was used for culturing of MDCK and Vero(B) cells. Culturing of the vaccine line Vero(B) in this medium was not accompanied by changes in proliferative activity and sensitivity to influenza viruses A(H1N1) and A(H3N2).
Subject(s)
Cell Culture Techniques , Culture Media , Influenza A Virus, H1N1 Subtype/physiology , Influenza A Virus, H3N2 Subtype/physiology , Oryza , Virus Replication , Animals , Cell Line , Cell Proliferation , Chlorocebus aethiops , Dogs , Protein Hydrolysates , Vero Cells , Virus CultivationABSTRACT
The reproduction of highly pathogenic avian influenza (HPAI) A/tern/SA/61 H5N3 and A/ducklNovosibirsk/56/05 H5NI viruses was comparatively studied in 16 human and animal cell lines. The strain A/duck/Novosibirsk/56/05 was shown to have a wider range of hosts. The most sensitive transplanted cell lines were found to be feline fibroblasts (CC-81), primarily trypsin-treated cells of chick embryonic fibroblasts (CEF), the kidney of dogs (MDCK), pigs (SPEV), monkeys (Vero), the human conjunctiva (1-5C-4), and, to a lesser extent, the feline kidney (CRFK). Unlike the strain A/tern/South Africa/61, that A/duck/Novosibirsk/56105 replicated in the polecat brain cells (Mpf).
Subject(s)
Influenza A virus/physiology , Animals , Cats , Cells, Cultured , Chick Embryo , Chlorocebus aethiops , Dogs , Humans , Influenza A Virus, H5N1 Subtype/growth & development , Orthomyxoviridae Infections/virology , Species Specificity , Swine , Vero Cells , Virus Cultivation , Virus ReplicationABSTRACT
Reproduction of parental strains and reassortants (with known genome composition) of influenza A and B viruses was studied in chick embryos (CE) and in different cell lines (SPEV, MDCK, BHK-21, M22, etc.). The results agree with the concept that the yield of influenza A virus in CE depends on its M-gene. At the same time, the experimental results suggest that reproduction of influenza B virus in the same system is not determined by M-gene. Reproduction (hr-phenotype) of influenza A and B viruses in cell cultures was shown to be determined not only by the gene coding for hemagglutinin but also by other virus genes, the reproduction level being dependent on different genes in different cell systems.
Subject(s)
Genome, Viral , Influenza A virus/physiology , Influenza B virus/physiology , Reassortant Viruses/physiology , Virus Replication/physiology , Animals , Cell Line , Humans , Influenza A virus/genetics , Influenza B virus/genetics , Reassortant Viruses/genetics , Virus Cultivation , Virus Replication/geneticsABSTRACT
The influence of the maintenance medium, polyethylene glycol (PEG), DEAE-dextran, and low temperature on reproduction of influenza A, B, and C viruses and their reassortants in diploid and continuous cell cultures was determined. Lowering of pH in the maintenance medium to 6.5 was found to decrease reproduction of influenza A (H1N1) and A (H3N2) viruses and increase that of influenza B viruses. Treatment of cells with PEG solution increased the yield of influenza B and C but not A viruses. However, influenza A virus strains proved to be capable of producing infectious progeny in nonpermissive cell lines treated with PEG. Addition of DEAE-dextran to the medium exerted no effect on the infectivity of influenza A and B reassortants. Moreover, infection of MDCK cells after a "cold shock" led to an increase in hemagglutinin titres in influenza A reassortants.
Subject(s)
Gammainfluenzavirus/physiology , Influenza A virus/physiology , Influenza B virus/physiology , Reassortant Viruses/physiology , Virus Replication/physiology , Animals , Cells, Cultured/microbiology , Chemical Phenomena , Chemistry, Physical , Culture Media , DEAE-Dextran/pharmacology , Humans , Hydrogen-Ion Concentration , Influenza A virus/drug effects , Influenza B virus/drug effects , Gammainfluenzavirus/drug effects , Polyethylene Glycols/pharmacology , Reassortant Viruses/drug effects , Temperature , Virus Cultivation , Virus Replication/drug effectsABSTRACT
The influence of mono-, di-, and trisialogangliosides on the dynamics of influenza B virus reproduction in human embryo fibroblast (HEF) cell culture and human diploid cells was established. The cells were treated with neuraminidase of non-cholera vibrio for removal of natural receptors followed by treatment with gangliosides. Virus reproduction was assessed by infectious titres for chick embryos and HA test of the culture fluid at certain intervals. Gangliosides restored influenza virus reception and enhanced the infectious process as compared with the controls. Treatment with gangliosides of HEF culture of low sensitivity increased its susceptibility to virus markedly.
Subject(s)
Gangliosides/pharmacology , Influenza B virus , Influenza, Human/microbiology , Receptors, Cell Surface , Adsorption , Cell Line , Cells, Cultured/drug effects , Cells, Cultured/microbiology , Dose-Response Relationship, Drug , Embryo, Mammalian , Humans , Influenza B virus/drug effects , Influenza B virus/pathogenicity , Influenza B virus/physiology , Neuraminidase/pharmacology , Receptors, Immunologic/drug effects , Vibrio/enzymology , Virus Cultivation/methods , Virus Replication/drug effectsABSTRACT
The study of reproductive activity of human and animal influenza A, B, and C viruses as well as influenza A virus reassortants in some cell cultures allowed one to determine the range of cells susceptible for each type (subtype) of the viruses. Differences in the range of cells were demonstrated for different strains of influenza viruses of the same antigenic subtype. It was noted that reassortants of influenza A viruses with the same hemagglutinin subtypes as the parental strains had a wider range of susceptible cell lines and a higher reproductive capacity in these cells.
