[Effect of isonicotinic acid derivates on reproduction of Epstein-Barr virus].

Current approaches to the treatment of herpes infection, particularly Epstein-Barr virus (EBV), include the use of etiotropic medicines, as well as sensitizing therapy. This virus plays an important role in the etiology of nasopharyngeal carcinoma, adenocarcinoma of the parotid glands, gastric carcinoma, Burkitt's lymphoma and lymphoproliferative syndromes [1, 2, 3]. The spectrum of drugs active against EBV remains very limited, and gancyclovir and acyclovir are used in medical practice, so the search of new compounds active against EBV remains urgent. The purpose of this work was to study antiEBV activity of isonicotinic acid derivatives in the cultures of lymphoblastoid Raji cells, B95-8, Namalwa. The indices of cytotoxicity (CC50) which amounted to 840, 1250 and 3000 microg/ml and the concentration of drugs, which inhibit the virus (IC50) reproduction is 0.1, 2.5 and 50 microg/ml, respectively, in cell cultures were identified. It was detected, the drug 4-(n-benzyl)aminocarbonyl-1-methylpyridinium iodide (PV-1) had an ability to inhibit reproduction of the Epstein-Barr virus in all studied cells cultures. The compounds PV-2 and PV-10 were less toxic in respect of the initial preparation PV-1, but their antiviral activity was manifested at 25 and 500 times higher concentrations. It, respectively, influenced the decrease of their selectivity index, which was 8400 for PV-1, 400 and 440--for PV-2 and PV-10. These studies suggest possible ways of further modification of the PV-1 molecule to create highly specific inhibitors of Epstein-Barr virus. The paper is presented in Ukrainian.

[Biochemical mechanisms of genome-protective activity of pyridine carbonic acid new derivatives with cells damaged by tetrachloromethane]. / Biokhimichni mekhanizmy henomozakhysnoï diï novykh pokhidnykh pirydynkarbonovykh kyslot za urazhennia klityn tetrakhlormetanom.

The biochemical mechanisms of genomoprotective action of the new derivatives of pyridinecarbonic acids--PV-3, PV-4 and PV-6 in the conditions of tetrachloromethane chemical cell damage have been studied. This effect is related to their antioxidant influence on LPO processes in transcriptionally active and repressed chromatin fractions of rat liver cells and partial restoration of these fractions structure which has been destroyed during intoxication. PV-4 possesses the most genoprotective activity among the substances studied.

Study of immunotropic activities of Bacillus subtilis as the basis of anti-scleroma probiotics.

Presence of influence of Bacillus subtilis during peroral and parenteral administration upon the laboratory animals' immune system has been studied. Absence of immuno-depressing action of the bacillus culture under study has been presented with the help of assessment tests on the humoral response to the thymus-dependent antigen: creation of the antibodies, and the delayed-onset hypersensitivity reaction. An insignificant stimulating effect has been revealed under execution of the reaction of the delayed-onset hypersensitivity as a result of B. subtilis application. The obtained data confirm the prospects of application of the B. subtilis strain as a basis of the anti-scleroma probiotics.

[Effect of amizon on structural modifications of liver nuclear chromatin from rats, intoxicated with tetrachloromethane]. / Vplyv amizonu na strukturnu modyfikatsiiu iadernoho khromatynu pechinky shchuriv, intoksykovanykh tetrakhlormetanom.

The actions of the amizon on the antioxidant activity and free radicaloformation level in the nuclear chromatin fractions of rat liver methan tetrachlorid intoxication was studied using the Fe(+2)-induction biochemiluminescence and microcalorimetry methods. The amizon genomoprotective effect on the chromatin transcriptionally active fraction was shown.